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3 protocols using nitrogen and oxygen gas cylinders

1

Synthesis and Characterization of 4-Vinylbenzophenone

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4-vinylbenzophenone (BP) was synthesized as previously published.30 (link) Sodium chloride (NaCl), potassium chloride (KCl), methacryloyloxyethyl phosphorylcholine (MPC), N-acetyl-D-penicillamine (NAP), ethylenediamine tetraacetic acid (EDTA), sodium phosphate dibasic (Na2HPO4), sodium phosphate monobasic (NaH2PO4), concentrated sulfuric acid (conc. H2SO4), tetrahydrofuran (THF), and sodium nitrite (NaNO2) were purchased from Sigma Aldrich (St. Louis, MO). 2–2’-azobis(2-methyl propionitrile (AIBN) and n-butyl methacrylate (BMA) were bought from Alfa-Aesar (Haverhill, MA). Concentrated hydrochloric acid (conc. HCl), methanol (CH3OH) and sodium hydroxide (NaOH) were obtained from Fisher Scientific (Hampton, NH). Potassium phosphate monobasic (KH2PO4) was purchased from BDH Chemicals-VWR International (West Chester, PA). CarboSil 20 80A UR STPU (referred to as CarboSil hereon) was obtained from DSM Biomedical Inc. (Berkeley, CA). De-ionized water (DI H2O) was obtained from in-house Milli-Q filtration system. The bacteria strains for the antimicrobial studies were obtained from American Type Culture Collection. Nitrogen and oxygen gas cylinders were from Air gas (Kennesaw, GA).
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2

Antibacterial Evaluation of CarboSil Polymer

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CarboSil® 2080A UR STPU (referred to as CarboSil hereon) was acquired from DSM Biomedical Inc. (Berkeley, CA). Anhydrous tetrahydrofuran (THF), N-acetyl-D-penicillamine (NAP), sodium nitrite (NaNO2), concentrated sulfuric acid (H2SO4), phosphate buffered saline (PBS), ZnO-NPs, cell counting kit-8 and ethylenediamine tetraacetic acid (EDTA) were obtained from Sigma Aldrich (St. Louis, MO). Concentrated hydrochloric acid (conc. HCl), and methanol were bought from Fisher-Scientific (Hampton, NH). CarboSil™ 2080A (CarboSil) was obtained from DSM Biomedical Inc. (Berkeley, CA). Gram-positive Staphylococcus aureus (ATCC 6538) and Gram-negative Pseudomonas aeruginosa (ATCC 27853, P. aeruginosa) were originally obtained from American Type Culture Collection (Manassas, VA). Milli-Q filter was used to obtain deionized (DI) water for all the aqueous solution preparations. Nitrogen and oxygen gas cylinders were purchased from Airgas (Kennesaw, GA). LB Agar (LA), Miller and Luria broth (LB), Lennox were purchased from Fischer BioReagents (Fair Lawn, NJ).
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3

Synthesis and Characterization of Biocompatible Materials

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Sodium nitrite (NaNO2), ethylenediaminetetraacetic acid (EDTA), tetrahydrofuran (THF), trioctyl trimellitate (TOTM), glutaraldehyde, and bis(trimethylsilyl)amine (HMDS) were purchased from Sigma-Aldrich (St. Louis, MO). Glutathione was purchased from GoldBio (St Louis, MO). DI water for all aqueous solutions was obtained using a Milli-Q filter from Millipore Corporation (Billerica, MA). Nitrogen and oxygen gas cylinders were purchased from Airgas (Kennesaw, GA). Hydrochloric acid (HCl), dimethyl sulfoxide (DMSO), and Saint-Gobain Tygon Formula S3 E-3603 poly(vinyl chloride) tubing were purchased from Fisher Scientific (Pittsburgh, PA). Acetone and ethanol were purchased from VWR (Radnor, PA). Drabkin’s reagent was obtained from Ricca Chemical (Arlington, TX). Phosphate-buffered saline (PBS), pH 7.4, supplemented with 138 mM NaCl, 2.7 mM KC, and 10 mM sodium phosphate, and 100 μM EDTA were used. Calcium- and magnesium-free PBS (CMF-PBS) was made with 138 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, and 1.47 mM KH2PO4 with H2O.
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