Inductively Coupled Plasma-Mass Spectroscopy (ICP-MS, 7700 Series; Agilent, Santa Clara, CA) was used to evaluate the presence of Cr in the dissolved target material and the solutions eluted from each column. Aliquots of each sample were taken and analyzed as described in Pyles et al.13 (link).
7700 series
Manufactured by Agilent Technologies
Sourced in United States, Japan, Germany
The 7700 Series is a family of inductively coupled plasma mass spectrometers (ICP-MS) manufactured by Agilent Technologies. The core function of the 7700 Series is to provide highly sensitive and accurate elemental analysis of a wide range of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Ferrozine method, by Horiba (2 mentions)
Whatman, by Cytiva (2 mentions)
Suprapur, by Merck Group (2 mentions)
Precellys 24, by Bertin Technologies (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Icp ms, by Agilent Technologies (1 mentions)
Whatman filter paper, by Cytiva (1 mentions)
Optima grade, by Thermo Fisher Scientific (1 mentions)
7700 series icp ms, by Agilent Technologies (1 mentions)
Icp ms is mix1 1, by AccuStandard (1 mentions)
Aristar, by BD (1 mentions)
Optima 5300 dv, by PerkinElmer (1 mentions)
8454 uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Tecnai osiris, by Thermo Fisher Scientific (1 mentions)
Esprit 1, by Bruker (1 mentions)
Av 400 mhz spectrometer, by Bruker (1 mentions)
0.45 μm membrane filter, by Cytiva (1 mentions)
Arm 200cf microscope, by JEOL (1 mentions)
Micro bca assay kit, by Thermo Fisher Scientific (1 mentions)
Suprapur, by Carl Roth (1 mentions)
51 protocols using 7700 series
1
ICP-MS Analysis of Chromium
2
Quantifying Glutathione and Iron
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Glutathione (GSH) was measured as previously described [9 (link)]. Tissue iron analysis was carried out essentially as previously described [23 (link)] but using an Agilent Technologies 7700 series inductively-coupled plasma mass spectrometer. This method measures total iron in the sample regardless of the form. Serum iron was measured using the ferrozine method (Pointe Scientific Inc.).
3
Quantifying Carboplatin Tissue Retention
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To analyse the tissue retention of carboplatin or carboplatin NP, brains were explanted and samples taken around the infusion point using a 3mm diameter sample corer. Samples were thawed and homogenised twice in aCSF at 5000rpm for 20 seconds using a Precellys 24 homogenizer (Bertin Technologies, USA). Tissue homogenates were subsequently diluted with water or nitric acid (2%, v/v) and analysed by ICP-MS detection using the Hydrogen mode (Agilent Technologies 7700 series) equipped with an autosampler (Agilent G3160B) for platinum content. All time points were carried out in triplicate with experimental values normalised against 0 hours control.
4
Determination of Lead in Biological Samples
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This study was approved by the Research Ethics Committee from the Guangzhou Center for Disease Control and Prevention, Guangdong, China. Informed consent was obtained from all participants. The ultrapure water (resistivity 18.2 MΩ·cm) used in sample and solution preparation was obtained using a Milli-Q water purification system (Millipore Synergy, Carrollton, GA, USA). The nitric acid and 30% hydrogen peroxide used in this study were of ultrapure grade. Lead contents were determined by an inductively coupled plasma mass spectrometer equipped with collision cells (Agilent 7700 Series, Tokyo, Japan) operating with high-purity argon (99.999%, Guangzhou Air Plant, Guangzhou, China). The sample introduction system was composed of a quartz cyclonic spray chamber and a micromist nebulizer connected by Tygon® tubes to the peristaltic pump of the inductively coupled plasma-mass spectrometry (ICP-MS, Agilent, Tokyo, Japan).
5
Plasma Copper Measurement Protocol
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Plasma copper concentrations were measured in the Ministry of Education Key Laboratory of Environment and Health at Tongji Medical College of Huazhong University of Science & Technology, using inductively coupled plasma mass spectrometry with an octopole-based collision/reaction cell (Agilent 7700 Series, Tokyo, Japan). We measured case and control specimens randomly in the daily measurement, with laboratory personnel blinded to the case–control status. For quality assurance, we measured metals in standard reference materials once in every 20 samples using certified reference material (ClinChek human plasma controls for trace elements no. 8883 and 8884). The values measured in the reference materials were confirmed to within the recommended range for copper. For no. 8883, we determined a concentration of 917 ± 67 μg/L (certified: 925 ± 185 μg/L), and for no. 8884, we measured 1,314 ± 114 μg/L (certified: 1,363 ± 273 μg/L). Both the intraassay and interassay coefficient of variation of plasma copper were <5%. The limit of detection was 0.009 μg/L, and all study participants had plasma copper levels above the limit of detection.
6
Trace Element Quantification by ICP-MS
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The concentrations of microelements were measured by inductively-coupled plasma mass spectrometry (ICP-MS, Agilent 7700 Series, Santa Clara, CA, USA) according to [26 (link)]. Values of Cd, Cr, Cu, Ni, Pb, and Zn are given in ppb, which equals µg/kg dry weight (DW). Translocation factor ((TF), element concentration in the shoot/element concentration in the root) was calculated according to [27 ].
7
Measuring Toxic Elements in Street Dust
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For measuring the concentration of toxic elements, 50 mg of the sieved street dust (< 20 µm) was mixed with 20 mL of aqua regia solution (5 mL 63% HNO3 and 15 mL 36% HCL). To prepare aqua regia, an analytical grade reagent (HNO3 and HCl) was employed, and Milli-Q water (Type 1) was used to make all of the solutions. The diluted suspension was heated using hotplate (ZOJIRUSHI, EA-DD10-TA) at 150 °C for 1 h 30 min until the evolution of reddish-brown fumes ceased. Then, the digest was reduced to an approximate volume of 1 mL or close to dryness and allowed to cool down to room temperature before adding 20 mL of 2% HNO3. Afterwards, samples were filtered through Whatman filter paper of 5C 110 mm in diameter (pore size: 11 μm) and stored in a refrigerator until analysis (Kabir et al. 2021b (link)). The concentrations of toxic elements in the final solution were measured by inductively coupled plasma mass spectrometry (ICP-MS) (Agilent Technologies, 7700 series, USA).
8
Quantifying Cellular Uptake of Gold Nanoparticles
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The intracellular uptake of gold nanoparticles was determined using ICP-MS analysis as reported earlier. Briefly, the cultured C6 cells post treatment with nanobioconjugates were trypsinised at different time points. The cells were further lysed using triton X. A total of 100 µL of samples was digested using 5% HNO3:HCl (3:1) solution at 70 °C. The ion content was estimated by ICP-MS (Agilent technologies 7700 series, Santa Clara, CA, USA) using a standard ion solution (Tracecert standards).
9
Determination of Urinary Metal Content
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The determination of metal contents in urine was performed as previously described [37 (link)], with minor modification. In brief, the frozen urine samples were completely thawed at room temperature and homogenized. A 3.0 ml aliquot of urine was transferred to a polypropylene tube (Jiayu experiment instrument Co., Ltd., Haimen, China) containing 15.0 μl of 67% (v/v) HNO3 and stored in a refrigerator at 5°C. Two hours before sample preparation the urine samples were brought to room temperature. A 1.0 ml of the sample was pipetted into a 10ml disposable polypropylene tube and then filled up to 5.0 ml with 1.2% (v/v) HNO3 (OptimaTM grade, Fisher, Belgium) using adjustable volume pipette samplers. The samples were then measured using an inductively coupled plasma mass spectrometry with an octopole based collision/reaction cell (Agilent 7700 Series, Waldbronn, USA).
10
Total Iron Measurement by ICP-MS
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Total iron was measured using a 7700 Series (Agilent) inductively coupled plasma mass spectrometry (ICP-MS) as previously reported.46 (link) Samples consisted of 100 adults per replicate for different aged cohorts as indicated.
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