Annexin v 7 aad apoptosis detection kit

Name: Annexin v 7 aad apoptosis detection kit
Brand: BD
SKU: -iThCZIBPBHhf-iF2jOe
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Manufactured by BD

47 citations

Sourced in United States, Germany

About the product

The Annexin V/7-AAD apoptosis detection kit is a laboratory tool used to identify and quantify apoptotic cells. It utilizes Annexin V, a protein that binds to phosphatidylserine, and 7-AAD, a DNA-binding dye, to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells.

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47 protocols using «annexin v 7 aad apoptosis detection kit»

Annexin V/7-AAD Apoptosis Assay

2024

Apoptosis was evaluated with the annexin V/7-AAD apoptosis detection kit (BD Biosciences) following the manufacturer’s protocol. Apoptosis was also demonstrated by the detection of protein cleavages with western blotting.

Chen Z., Vallega K.A., Wang D., Quan Z., Fan S., Wang Q., Leal T., Ramalingam S.S, & Sun S.Y. (2024). Inhibition of hTERT/telomerase/telomere mediates therapeutic efficacy of osimertinib in EGFR mutant lung cancer. The Journal of Experimental Medicine, 221(11), e20240435.

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Annexin V/7-AAD Apoptosis Assay

2024

Detection of apoptosis was done with the annexin V/7‐AAD apoptosis detection kit (BD Biosciences; San Jose, CA) according to the manufacturer's instructions. Apoptosis was also demonstrated by protein cleavages detected with Western blotting.

Chen Z., Vallega K.A., Boda V.K., Quan Z., Wang D., Fan S., Wang Q., Ramalingam S.S., Li W, & Sun S. (2024). Targeting Transient Receptor Potential Melastatin‐2 (TRPM2) Enhances Therapeutic Efficacy of Third Generation EGFR Inhibitors against EGFR Mutant Lung Cancer. Advanced Science, 11(35), 2310126.

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Multiparametric Evaluation of Cellular Responses

2024

Cell death: Programed cell death (PCD) levels were evaluated by measuring the sum of apoptotic and necrotic cells using dual staining with APC-conjugated annexin-V and 7-AAD using the Annexin V/7-AAD Apoptosis Detection Kit (BD Biosciences). Staurosporine (STS) at 1 μM concentration was used as cell death positive control.

CD4, CCR5, and CXCR4 expression on LX-2 cells: It was analyzed through staining with specific antibodies APC-labeled anti-human CCR5 (1:5 #cat. ab176536, Abcam), PE-labeled anti-human CXCR4 antibody (1:5 #cat. 555974 BD Pharmingen), and PerCP-labeled anti-human CD4 (1:100 #cat. 344624 BioLegend) and flow cytometry.

Reactive oxygen species (ROS) production:

Total cellular ROS (tROS) production: Assessed using the 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA, Abcam) assay for total cellular ROS production, including hydroxyl, peroxyl, and other ROS. Cells were incubated with DCFDA at 10 µM in essential medium at 37°C for 45 min and then evaluated by flow cytometry. Tert-butyl hydroperoxide solution (TBH) at 100 μM concentration was used for tROS production as positive control.

Mitochondrial ROS (mROS) generation: Quantified by flow cytometry in cells stained by 5 µM MitoSOX™ (ThermoFisher Scientific) for 15 min. Rotenone at 10 μM concentration was used for mROS production as positive control.

TGF-β producing cells: LX-2 cells were fixed, permeabilized, and stained to detect TGF-β1 production (1:10, #cat. 562339 BD Pharmingen).

Flow cytometry measurements were carried out using a FACSCanto (BD Biosciences), and data analysis was conducted using FlowJo X software (TreeStar).

Cevallos C., Jarmoluk P., Sviercz F., López C.A., Freiberger R.N., Delpino M.V, & Quarleri J. (2024). Bystander Effects and Profibrotic Interactions in Hepatic Stellate Cells during HIV and HCV Coinfection. Journal of Immunology Research, 2024, 6343757.

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Corresponding organizations : Instituto de Investigaciones Biomédicas en Retrovirus y Sida, Consejo Nacional de Investigaciones Científicas y Técnicas, University of Buenos Aires

Annexin V/7-AAD Apoptosis Assay

2024

Apoptosis was evaluated with the annexin V/7-AAD apoptosis detection kit (BD Biosciences) according to the manufacturer’s protocol. Apoptosis was also demonstrated by protein cleavages detected with Western blotting.

Chen Z., Vallega K.A., Wang D., Quan Z., Fan S., Wang Q., Leal T., Ramalingam S.S, & Sun S.Y. (2024). DNA topoisomerase II inhibition potentiates osimertinib’s therapeutic efficacy in EGFR-mutant non–small cell lung cancer models. The Journal of Clinical Investigation, 134(10), e172716.

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Corresponding organizations : Emory University, Second Xiangya Hospital of Central South University, Central South University, Zhengzhou University, Henan Cancer Hospital

Annexin V-based Apoptosis Assay

2023

The effects of the given drug treatments on apoptosis were evaluated by annexin V staining/flow cytometry using the annexin V/7-AAD Apoptosis Detection Kit (BD Biosciences; Franklin Lakes, NJ) according to the manufacturer's instructions. Detection of protein cleavage by Western blot analysis was also used as an additional indication of apoptosis.

Zhao W., Yu D., Zhai Y, & Sun S.Y. (2023). ALK inhibitors downregulate the expression of death receptor 4 in ALK-mutant lung cancer cells via facilitating Fra-1 and c-Jun degradation and subsequent AP-1 suppression. Neoplasia (New York, N.Y.), 42, 100908.

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Corresponding organizations : Emory University

Top 5 most cited protocols using «annexin v 7 aad apoptosis detection kit»

Cell Death Induction by PTL and DMAPT

Cited 3 times

Cell division tracking was performed with carboxy‐fluorescein diacetate succinidmidyl diester (CFSE). Cells were stained with 1 mmol/L CFSE and cultured in the presence or absence of PTL or DMAPT. After 24 hours, cells were harvested, washed and analysed by flow cytometry. Cell death was determined by staining with YoPro‐1 and 7AAD, or Annexin‐V/7AAD apoptosis detection kit (BD Biosciences) and flow cytometry analysis. The apoptotic index was determined by calculating the fold change in apoptotic cells (Yopro‐1/7AAD or Annexin‐V/7AAD positive) in PTL or DMAPT treated cells compared to the death cells in Untreated groups (considered as 1). Caspase dependent cell death was determined by pre‐treatment of cells with Z‐vad (100μmol/L) for 2 hours previous to PTL and DMAPT treatment. Cell viability was determined after 24 hours and caspase 3 activation by detection of the active form of caspase 3 (C92‐605, BD Biosciences, Mountain View, CA) by flow cytometry.

Flores‐Lopez G., Moreno‐Lorenzana D., Ayala‐Sanchez M., Aviles‐Vazquez S., Torres‐Martinez H., Crooks P.A., Guzman M.L., Mayani H, & Chávez‐González A. (2018). Parthenolide and DMAPT induce cell death in primitive CML cells through reactive oxygen species. Journal of Cellular and Molecular Medicine, 22(10), 4899-4912.

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Corresponding organizations : Mexican Social Security Institute, University of Arkansas for Medical Sciences, Cornell University

Cytotoxicity and Chemoprotection Assays for AML

Cited 2 times

The cytotoxic effect of the different drugs on HL-60 and MOLM-13 cell was assessed using Cell Counting Kit-8 based on monosodium salt WST-8 according to the manufacturer’s protocol (Sigma-Aldrich). Briefly, 5.000 cells were plated in 96-well plate and incubated with increasing concentrations of the corresponding drug for 48h. After incubation time, absorbance was measured at 450 nm using a microplate reader. The cytotoxic effect of IMiDs on BM-MSCs was assessed using MTT assays as previously described.^21,22 Briefly, 2 × 10⁴ cells were cultured with increasing drug concentrations in 96-well plates for 48 h. To determine the chemoprotective effect of BM-MSCs a total of 2 × 10⁴ BM-MSCs were plated in 96-well plates 18 h before addition of AML cells (2 × 10⁴ for cell lines and 2 × 10⁵ for primary cells). BM-MSC:AML co-cultures were treated with IC₂₅ concentrations of the AraC (77nM) and Idarubicin (7nM) and 10 µM of lenalidomide/pomalidomide for 48–72 h. Apoptosis of CD33⁺ AML cells was measured using the annexin-V/7-AAD apoptosis detection kit (BD Biosciences) on a FACSCanto-II cytometer using FACSDiva software (BD Biosciences) as previously described.²³

Lopez-Millan B., Diaz de la Guardia R., Roca-Ho H., Anguita E., Islam A.B., Romero-Moya D., Prieto C., Gutierrez-Agüera F., Bejarano-Garcia J.A., Perez-Simon J.A., Costales P., Rovira M., Marín P., Menendez S., Iglesias M., Fuster J.L., Urbano-Ispizua A., Anjos-Afonso F., Bueno C, & Menendez P. (2018). IMiDs mobilize acute myeloid leukemia blasts to peripheral blood through downregulation of CXCR4 but fail to potentiate AraC/Idarubicin activity in preclinical models of non del5q/5q- AML. Oncoimmunology, 7(9), e1477460.

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Corresponding organizations : Josep Carreras Leukaemia Research Institute, Universitat de Barcelona, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Universidad Complutense de Madrid, University of Dhaka, Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, Universidad de Sevilla, EntreChem (Spain), Hospital Clínic de Barcelona, Hospital Del Mar, Hospital Universitario Virgen de la Arrixaca, Centro de Investigación Biomédica en Red de Cáncer, Instituto de Salud Carlos III, Institució Catalana de Recerca i Estudis Avançats

Apoptosis Detection via Annexin V/7-AAD

Cited 1 time

Apoptosis was evaluated with an annexin V/7-AAD apoptosis detection kit (BD Biosciences; San Jose, CA) according to the manufacturer's instructions. Caspase and PARP cleavage were also detected by Western blot analysis as additional indicators of apoptosis.

Shi P., Oh Y.T., Deng L., Zhang G., Qian G., Zhang S., Ren H., Wu G., Legendre B J.r., Anderson E., Ramalingam S.S., Owonikoko T.K., Chen M, & Sun S.Y. (2017). Overcoming acquired resistance to AZD9291, a third generation EGFR inhibitor, through modulation of MEK/ERK-dependent Bim and Mcl-1 degradation. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(21), 6567-6579.

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Corresponding organizations : First Affiliated Hospital of Xi'an Jiaotong University, Emory University, Transgenomic (United States)

Phagocytosis of Apoptotic Thymocytes

Cited 1 time

Single-cell thymocyte suspensions were made by pushing thymi from 4–6 weeks old B6 mice through cell strainers (50μm). After washing twice with PBS, cells were labeled with CFSE (Invitrogen, Carlsbad, CA), and then rendered apoptotic by exposure to 500 Rads of γ-irradiation followed by 4 hrs culture in complete RPMI medium. Apoptosis was verified using an Annexin-V/7-AAD Apoptosis detection kit (BD Pharmingen, San Diego, CA) [3 (link)]. Apoptotic thymocytes were co-cultured for 4 hrs with phagocytes at various ratios as indicated. The phagocytes were extensively washed with PBS containing EDTA to remove physically bound but not internalized apoptotic cells. Percentages of macrophages that had ingested labeled thymocytes were determined by FACS analysis, with gating on CD11b-PE and CFSE.

Shao W.H., Zhen Y., Eisenberg R.A, & Cohen P.L. (2009). The Mer receptor tyrosine kinase is expressed on discrete macrophage subpopulations and mainly uses Gas6 as its ligand for uptake of apoptotic cells. Clinical immunology (Orlando, Fla.), 133(1), 138-144.

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Corresponding organizations : Temple University, University of Pennsylvania

Apoptosis Evaluation by Flow Cytometry

Cited 1 time

Apoptosis was evaluated with an Annexin V/7-AAD apoptosis detection kit (BD Biosciences; San Jose, CA) according to the manufacturer's instructions. Caspase and PARP cleavage were also detected by Western blot analysis as additional indicators of apoptosis.

Zhang S., Chen Z., Shi P., Fan S., He Y., Wang Q., Li Y., Ramalingam S.S., Owonikoko T.K, & Sun S.Y. (2021). Downregulation of death receptor 4 is tightly associated with positive response of EGFR mutant lung cancer to EGFR-targeted therapy and improved prognosis. Theranostics, 11(8), 3964-3980.

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Corresponding organizations : Emory University, Central South University, Second Xiangya Hospital of Central South University, Daping Hospital, Zhengzhou University, Henan Cancer Hospital

Spelling variants (same manufacturer)

7-AAD - 2174 citations Apoptosis detection kit - 523 citations Annexin V/7-AAD - 56 citations Annexin V/7-AAD kit - 21 citations PE Annexin V/7-AAD apoptosis detection kit - 19 citations Annexin V/7-AAD apoptosis kit - 13 citations Apoptosis kits - 5 citations Annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit - 5 citations FITC-Annexin V/7-AAD apoptosis detection kit - 5 citations PE Annexin-V/7-AAD Apoptosis Detection Kit I - 4 citations

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The spelling variants listed above correspond to different ways the product may be referred to in scientific literature.
These variants have been automatically detected by our extraction engine, which groups similar formulations based on semantic similarity.

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