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13c2 pfhxa

Manufactured by Wellington Laboratories
Sourced in Canada
About the product

13C2-PFHxA is a chemical standard used for analytical purposes. It serves as a reference compound for the identification and quantification of perfluorohexanoic acid (PFHxA) in various environmental and analytical samples.

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9 protocols using «13c2 pfhxa»

1

Comprehensive PFAS Mixture Analysis Protocol

2022
PFAC-MXB, a native PFAS mixture standard solution, was purchased from Wellington Laboratories Inc. (Guelph, ON, Canada). Perfluorobutane sulfonic acid (PFBS), perfluorohexanesulfonic acid (PFHxS), perfluorooctanesulfonic acid (PFOS), perfluorodecanesulfonic acid (PFDS), perfluorobutanoic acid (PFBA), perfluoropentanoic acid (PFPeA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUnDA), perfluorodecanoic acid (PFDoDA), perfluorotridecanoic acid (PFTrDA), perfluorotetradecanoic acid (PFTeDA), perfluorohexadecanoic acid (PFHxDA), perfluorooctadecanoic acid (PFOcDA), individual native standards, perfluorooctane sulfonamide (FOSA), N-ethyl perfluorooctane sulfonamide (N-EtFOSA), and N-ethyl perfluorooctane sulfonamide acetic acid (N-EtFOSAA) were procured from the same source.
The mass-labeled standard mixtures MPFAC-MXA, consisting of 18O2-PFHxS, 13C4-PFOS, 13C4-PFBA, 13C2-PFHxA, 13C4-PFOA, 13C5-PFNA, 13C2-PFDA, 13C2-PFUnDA, and 13C2-PFDoDA, and individual mass-labeled standards, 13C3-PFBS and 13C5-PFPeA, were purchased from Wellington Laboratories Inc. (Guelph, ON, Canada). All compounds had >98% purity.
Oasis® weak anion exchange (WAX; 6 cc, 150 mg, 30 μm) solid-phase extraction (SPE) cartridges (Waters Corp., Milford, MA, USA), Supelclean ENVI-Carb (100 mg, 1 mL) cartridges (Sigma-Aldrich Corp., St. Louis, MO, USA), ammonium acetate (97%; Wako Pure Chemical Industries Ltd., Osaka, Japan), ammonium solution (25%; Wako Pure Chemical Industries Ltd., Osaka, Japan), acetic acid (>99.7%; guaranteed reagent; Wako Pure Chemical Industries Ltd., Osaka, Japan), ultrapure water (prepared with a Milli-Q® gradient system; Millipore Co., Bedford, MA, USA), and methanol (>99.8%; pesticide residue-PCB analytical grade; Wako Pure Chemical Industries Ltd., Osaka, Japan) were used in all experiments and instrumental analyses.
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2

Quantification of Perfluorinated Compounds

2022
Perfluorooctane sulfonamide (PFOSA); a mixture of PFBA, perfluoro-n-pentanoic acid (PFPeA), perfluoro-n-hexanoic acid (PFHxA), perfluoro-n-heptanoic acid (PFHpA), PFOA, perfluoro-n-nonanoic acid (PFNA), perfluoro-n-decanoic acid (PFDA), perfluoro-n-undecanoic acid (PFUnDA), perfluoro-n-dodecanoic acid (PFDoDA), perfluoro-n-tetradecanoic acid (PFTrDA), perfluoro-n-tetradecanoic acid (PFTeDA), perfluoro-n-hexadecanoic acid (PFHxDA), perfluoro-n-octadecanoic acid (PFOcDA), potassium perfluoro-1-butanesulfonate (PFBS), PFHxS, PFOS, and sodium perfluoro-1-decanesulfonate (PFDS); and mixture of 13C4-PFOS, 13C2-PFDA, 13C5-PFNA, 13C4-PFOA, 13C4-PFBA, 13C2PFHxA, 13C2PFUnDA, 13C2PFDoDA, and 18O2PFHxS were purchased from Wellington Laboratories Inc. (Guelph, Ontario, Canada). Purities of the individual chemicals and mass-labeled chemicals are >98%. Oasis weak anion exchange solid-phase extraction cartridges (WAX; 6cc, 150 mg) were purchased from Waters Corp. (Milford, MA, USA). Methanol (LC-MS grade) was purchased from Merck Ltd. (Darmstadt, Germany), and ammonium solution (25%) was purchased from Sigma-Aldrich GmbH (Seelze, Germany). Milli-Q water was used during the experiment.
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3

Quantitative Analysis of PFAAs

2021
A mixed standard of twelve PFAAs (PFAC-MXB Stock Solution) including nine PFCAs, perfluoro-n-butanoic acid (PFBA), perfluoro-n-pentanoic acid (PFPeA), perfluoro-n-hexanoic acid (PFHxA), perfluoro-n-heptanoic acid (PFHpA), perfluoro-noctanoic acid (PFOA), perfluoro-n-nonanoic acid (PFNA), perfluoro-n-decanoic acid (PFDA), perfluoro-n-undecanoic acid (PFUnDA), perfluoro-n-dodecanoic acid (PFDoDA), and three PFSAs, perfluoro-1-butanesulfonate (PFBS), perfluoro-1hexanesulfonate (PFHxS), perfluoro-1-octanesulfonate (PFOS), with equal concentrations, was purchased from Wellington Laboratories, Inc. (Guelph, Ontario, Canada). Stable isotope labelled PFCAs and PFSAs used as internal standard compounds (SIL-IS) ( 13 C4-PFBA, 13 C5-PFPeA, 13 C2-PFHxA, 13 C2-PFHxA, 13 C4-PFOA, 13 C5-PFNA, 13 C2-PFDA, 13 C2-PFUnDA, 13 C2-PFDoDA, 18 O2-PFHxS, and 13 C4-PFOS) were purchased from Wellington Laboratories (Guelph, ON, Canada) in solution mixtures (masslabelled MPFAC-MXA solution at 2 µg mL -1 and mass-labelled M3PFPeA solution at 50 µg mL -1 ). The phospholipid removal solid-phase extraction (SPE) cartridges (Phree™) (30 mg, 1 mL SPE Tubes) were obtained by Phenomenex (Torrance, California, USA). Hybrid-SPE cartridges were prewashed with 3 mL of acetonitrile (ACN) containing 0.05 % of formic acid according to Honda et al. 22 . All reagents were analytical reagent grade. LC-MS grade Chromasolv methanol (MeOH), LC-MS grade Chromasolv ACN, ammonium acetate (99%), and concentrated formic acid were purchased from Sigma-Aldrich. Water (<18MΩcm resistivity) was produced by a Millipore Direct-QUV water purification system (Millipore, Bedford, MA, USA). Sodium chloride (NaCl) and anhydrous magnesium sulfate MgSO4 (purity ≥99.0%) were purchased from Carlo Erba (Milan, Italy) and Honeywell (Charlotte, North Carolina, USA), respectively.
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4

Quantitative Analysis of Perfluoroalkyl Substances

2020
Twelve PFASs (standards purchased from
Sigma-Aldrich) were analyzed, i.e., C3–C5 and C7–C9 perfluoroalkyl carboxylates
(PFCAs), C4, C6, and C8 perfluoroalkyl
sulfonates (PFSAs), perfluorooctane sulfonamide (FOSA), and 6:2 and
8:2 fluorotelomer sulfonates (6:2 and 8:2 FTSA). For quantification
as well as for quality assurance and control (QA/QC), eight mass-labeled
internal standards (ISs) (i.e., 13C4-PFBA, 13C2-PFHxA, 13C4-PFOA, 13C5-PFNA, 13C2-PFDA, 18O2-PFHxS, 13C4-PFOS, 13C8-FOSA, purities > 99%, Wellington Laboratories,
Guelph, ON) were also included. For details on the included PFASs
and their internal standards, see Table S1 in the Supporting Information.
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5

Quantifying Perfluorinated Compounds in Samples

2020
The target PFASs comprised: six perfluoroalkyl carboxylates (PFCAs), namely perfluorobutanoate (PFBA), perfluorohexanoate (PFHxA), perfluorooctanoate (PFOA), perfluorononanoate (PFNA), perfluorodecanoate (PFDA), and perfluoroundecanoate (PFUnDA); two perfluoroalkyl sulfonates (PFSAs), namely perfluorohexane sulfonates (PFHxS) and perfluorooctane sulfonates (PFOS); and one perfluorooctanesulfonamide (FOSA) (purity >99%, Wellington Laboratories, Guelph, ON). A total of nine isotopically labeled internal standards (IS) were used: 13C4-PFBA, 13C2-PFHxA, 13C4-PFOA, 13C5-PFNA, 13C2-PFDA, 13C2-PFUnDA, 18O2-PFHxS, 13C4-PFOS, and 13C8-FOSA (purity >99%, Wellington Laboratories, Guelph, ON).
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