Lysates of lentivirus-infected cells were prepared by standard methods. Western blot analysis was then performed by incubating the filter with mouse anti-NIS (1:500; Neomarker), anti-His-tag (1:500; Neomarker) and anti-GAPDH (1:10000; Abgent) antibody in Tris-buffered saline Tween-20 overnight at 4°C, followed by incubation with peroxidase-conjugated goat anti-mouse immunoglobulin G (1:2500; Neomarker) for 1 h at room temperature. Immunodetection was carried out using an ECL Western blot detection kit (Pierce).
Anti gapdh
Anti-GAPDH is a laboratory reagent used to detect the presence and abundance of the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) protein in biological samples. GAPDH is a commonly used housekeeping gene and its expression is often analyzed as a reference point in various research applications.
Lab products found in correlation
11 protocols using anti gapdh
Quantification of VEGF Expression in Engineered Cells
Lysates of lentivirus-infected cells were prepared by standard methods. Western blot analysis was then performed by incubating the filter with mouse anti-NIS (1:500; Neomarker), anti-His-tag (1:500; Neomarker) and anti-GAPDH (1:10000; Abgent) antibody in Tris-buffered saline Tween-20 overnight at 4°C, followed by incubation with peroxidase-conjugated goat anti-mouse immunoglobulin G (1:2500; Neomarker) for 1 h at room temperature. Immunodetection was carried out using an ECL Western blot detection kit (Pierce).
Phospho-protein Detection Protocol
Western Blot Analysis of β-Catenin, HIPK2, and LAMINB1
Protein Extraction and Western Blot Analysis
Western Blot of Baculovirus-Expressed Proteins
Immunoblotting Analysis of Signaling Pathways
Western Blot Analysis of Protein Signaling
Protein Expression Analysis by Western Blot
Western Blot Analyses of Signaling Proteins
Immunoblotting Analysis of Cellular Signaling
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