Mini-extruder
The Mini-extruder is a compact laboratory instrument designed for small-scale extrusion processes. It features a simple and intuitive operation for controlled material processing.
Lab products found in correlation
17 protocols using Mini-extruder
Liposome Preparation and Characterization
Preparation of Lipid-Based Nanoliposomes
Optimized Encapsulation of P5 Peptide in Liposomes
Macrophage Membrane-Coated PCOD@PLGA Nanoparticles
Preparation of Large Unilamellar Vesicles
(LUVs) composed of a mixture of zwitterionic and negatively charged
lipids (DMPC and DMPS in 3:1 ratio) or the brain total lipid extract
(bTLE). Model membranes were prepared as described elsewhere.85 (link) Briefly, appropriate aliquots of lipid stock
solutions in chloroform were dried by using a stream of dry nitrogen
and evaporated overnight under high vacuum to dryness in a round-bottomed
flask. Initially, multilamellar vesicles (MLVs) were obtained by hydrating
the lipid film with an appropriate amount of buffer (aCSF buffer 10
mM (pH 7.4) or MOPS buffer 10 mM and 100 mM NaCl (pH 7.4)) and dispersing
by vigorous stirring. MLVs were then extruded through polycarbonate
filters (pore size = 100 nm, Nuclepore, Pleasanton, CA) mounted in
a mini-extruder (Avestin, Ottawa, Canada) fitted with two 0.5 mL Hamilton
gastight syringes (Hamilton, Reno, NV) to obtain LUVs. Samples were
typically subjected to 23 passes through two filters in tandem and
as recommended elsewhere.86 (link)
Preparation of Large Unilamellar Lipid Vesicles
Fabrication of MM/RAPNP Nanocomposites
OEM-ETBPD Synthesis by Co-extrusion
Preparation of Large Unilamellar Vesicles
Preparation of RBC-LVTNP Hybrid Vesicles
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