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Standard kit

Manufactured by Asan Pharmaceutical
Sourced in Cameroon

The Standard kit is a collection of essential lab equipment designed for general laboratory use. It includes common tools and instruments necessary for various scientific experiments and procedures. The kit provides a basic set of equipment to facilitate standard laboratory work.

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6 protocols using Standard kit

1

Evaluation of Anti-Inflammatory Peptide in Bacterial Infection

Twenty ICR mice were randomly divided in to 4 groups (5 mice per group). PBS alone mice served as normal control. Peptide control mice received i.p. injections of R-Pro0-3D (1 mg/kg). Mice received only CRAB C0 (6 × 106 CFU/mice) act as bacterial control. For peptide treatment groups, R-Pro9-3D was injected 1 h before CRAB C0 injection. After 16 h of treatment, mice were killed by euthanasia and the lungs, liver, and kidneys were removed aseptically and then homogenized using ice-cold PBS. To assess the relative abundance of CRAB C0, all homogenates (1:1000, PBS) were plated onto Luria–Bertani agar, and the numbers of bacteria colonies were counted [42 (link)]. The levels of inflammatory cytokines (TNF-α and IL-6) were measured in the serum and lung lysates using corresponding ELISA kits (R&D Systems, Minneapolis, MN, USA). The contents of AST, ALT, and BUN were determined using a standard kit from Asan Pharmaceutical, as described previously [74 (link)]. To determine microanatomical features of polymorpho-neutrophil infiltrations, 5 μm-thick sections were prepared from paraffin blocked lungs and sequentially processed for hematoxylin and eosin (H&E) staining and examined using light microscope.
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ICR mice were randomly divided in to six groups (5 mice per group). PBS alone served as the vehicle. Peptide control mice received i.p. injections of papiliocin or PMB (1 mg/kg in PBS). For peptide treatment groups, papiliocin or PMB (1 mg/kg) was i.p. injected 1 h before E. coli K1 injection (5 × 106 CFU/mice). At the time of killing, the lungs, liver, and kidneys were removed aseptically and were then homogenized using ice-cold PBS. To assess the relative abundance of E. coli, all homogenates (1:1,000, PBS) were plated onto Luria–Bertani agar, and the numbers of bacteria colonies were counted (56 ). The levels of inflammatory cytokines (TNF-α and IL-6) were measured in the serum and lung lysates using corresponding ELISA kits (R&D Systems). The contents of AST, ALT, and BUN were determined using a standard kit from Asan Pharmaceutical, as described previously (37 (link)). To estimate polymorphonuclear leukocyte (PMN) infiltration levels, 4-μm-thick sections were prepared from paraffin-blocked lungs and sequentially processed for hematoxylin and eosin staining and examined using a light microscope.
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The detection of aspartate aminotransferase (AST), alanine amino transferase (ALT) and blood urea nitrogen (BUN) in the serum was performed using a standard kit available from Asan Pharmaceutical, (Gyeonggi-do, South Korea) as per the manufacturer’s instructions. The levels of AST, ALT and BUN were determined relative to a standard provided by the kit after subtracting the background levels. PBS was used as the negative control and a standard solution was used as the positive control to calculate the rates.
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BALB/c mice were used to induce sepsis by infection with E.
coli K1 to evaluate the therapeutic efficacy of Pap12-7. The mice were purchased from Orient (Korea) and maintained under specific pathogen-free conditions for one week before the experiment in a humidity-and temperature-controlled environment. Analysis of the sepsis model was conducted as described previously [22] . The amount of TNF-α and IL-6 in the serum and lung lysate of the mice was measured using sandwich ELISA kits. Aspartate aminotransferase (AST), alanine amino transferase (ALT), and blood urea nitrogen (BUN) levels in the serum were measured using the standard kit from Asan Pharmaceutical as described previously [29] . The lungs were extracted and fixed with 4% paraformaldehyde solution, stained with hematoxylin and eosin, and observed under a microscope.
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Aspartate aminotransferase (AST), alanine amino transferase (ALT), and blood urea nitrogen (BUN) in the serum were measured using standard kits available from Asan Pharmaceutical (Seoul, Korea) following previously described methods46 (link).
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ICR mice were randomly divided into four groups (three mice per group). Mock-treated “normal” animals were i.p. injected with PBS alone. The peptide control group was injected with T. ni cecropin (1 mg/kg); the LPS control group received LPS O127:B8 (18 mg/kg; Sigma-Aldrich). In the peptide treatment group, T. ni cecropin was injected 1 h prior to LPS injection. At 16 h post-injection, mice were euthanized, and serum was obtained for measurement of the levels of inflammatory cytokines IL-6 using ELISA kits (R&D Systems). The aspartate aminotransferase (AST), alanine aminotransferase (ALT), and blood urea nitrogen (BUN) levels in the serum were measured using standard kits from Asan Pharmaceutical (Seoul, Republic of Korea), as described previously [55 (link)].
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