Spa70

Q: How does spa70 by WuXi AppTec differentiate itself in the laboratory equipment market?

spa70 stands out through its advanced research capabilities, precision engineering, and high-performance specifications. While alternative products from vendors like Merck Group, Thermo Fisher Scientific, and PerkinElmer exist in the market, spa70 offers unique design features tailored for sophisticated laboratory workflows.

Q: What citation details should researchers use when referencing spa70 in scientific publications?

When citing spa70, researchers should include the full product name, manufacturer (WuXi AppTec), specific catalog number, and version identifier. Recommended citation format typically includes manufacturer details to ensure precise scientific traceability.

Q: What laboratory systems and accessories are compatible with spa70?

spa70 integrates seamlessly with complementary research tools, including equipment from correlated product lines such as Promega's reporting systems, PerkinElmer's plate readers, and Thermo Fisher Scientific's cell culture accessories. Its versatile design supports multiple experimental configurations.

Q: What primary research domains utilize spa70?

spa70 is predominantly utilized in advanced pharmaceutical research, drug discovery, molecular biology, and biochemical screening processes. Its precision makes it particularly valuable in high-throughput screening, protein interaction studies, and complex experimental protocols.

Q: What unexpected scientific insight is associated with spa70?

spa70 has been instrumental in breakthrough research involving complex protein interaction mapping, enabling researchers to explore molecular mechanisms with unprecedented resolution and efficiency, potentially accelerating therapeutic development strategies.

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About the product

The SPA70 is a laboratory instrument designed for conducting scintillation proximity assays (SPA). It is capable of detecting and measuring radioactive signals generated during these assays, which are commonly used in drug discovery and biochemical research applications. The SPA70 provides a means of quantifying interactions between biomolecules, such as proteins and small molecules, without the need for physical separation.

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Lab products found in correlation

T0901317, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Rifampicin, by Merck Group (2 mentions) Birabresib, by MedChemExpress (1 mentions) Unfused gst protein, by Abcam (1 mentions) Flag rxrα protein, by Active Motif (1 mentions) Mouse anti flag m2 fitc antibody, by Merck Group (1 mentions) Mouse anti pxr h 11 antibody, by Santa Cruz Biotechnology (1 mentions) Cpi 203, by MedChemExpress (1 mentions) Pregnenolone 16α carbonitrile pcn, by Merck Group (1 mentions) Rabbit anti β actin antibody, by Cell Signaling Technology (1 mentions) Dual glo luciferase reporter assay reagent, by Promega (1 mentions) 96 well white tissue culture treated plates, by PerkinElmer (1 mentions) Rifampicin rif, by Bio-Techne (1 mentions) Steadylite hts reagent, by PerkinElmer (1 mentions) Opti mem reduced serum medium, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Disposable pipettes, by Corning (1 mentions) Fugene 6, by Promega (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)

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Similar products (other manufacturers)

SPA70 (by Axon Medchem) - 5 citations

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Product FAQ

How does spa70 by WuXi AppTec differentiate itself in the laboratory equipment market?

What citation details should researchers use when referencing spa70 in scientific publications?

What laboratory systems and accessories are compatible with spa70?

What primary research domains utilize spa70?

What unexpected scientific insight is associated with spa70?

3 protocols using «spa70»

Ligand-Induced PXR Activation Assay

2023

DMSO, rifampicin, T0901317, and pregnenolone-16α-carbonitrile (PCN) were purchased from Sigma-Aldrich. SPA70 was obtained from WuXi AppTec (22 (link)). (+)-JQ1, (−)-JQ1, birabresib, and CPI-203 were purchased from MedChemExpress. All compound purities were determined to be >95% by the manufacturers. LanthaScreen Terbium (Tb)-anti–glutathione S-transferase (GST, cat. # PV3550) and GST-PXR LBD protein (cat. # PV4841) were purchased from Thermo Fisher Scientific. Unfused GST protein was purchased from Abcam (cat. # ab70456). FLAG-RXRα protein was purchased from Active Motif (cat. # 81782). Mouse anti-FLAG M2-FITC antibody was purchased from Sigma-Aldrich (cat. # F4049). Unlabeled and FAM-labeled SRC-1 peptides (N-CPSSHSSLTERHKILHRLLQEGSPS-C) were prepared by the Hartwell Center Macromolecular Synthesis Section at St. Jude Children's Research Hospital (23 (link)). FAM-labeled NCoR peptide (N-DPASNLGLEDIIRKALMGSFDDK-C) was also prepared by the Hartwell Center Macromolecular Synthesis Section at St. Jude Children's Research Hospital (22 (link)). BODIPY FL vindoline was synthesized as previously reported (24 (link)). Mouse anti-PXR (H-11) antibody was purchased from Santa Cruz Biotechnology (cat. # sc-48340). Rabbit anti-β-actin antibody was purchased from Cell Signaling Technology (cat. # 4967L). Goat anti-mouse IRDye 800CW (cat. # 926–32210) and goat anti-rabbit IRDye 680LT (cat. # 926–68021) secondary antibodies were purchased from LI-COR.

Huber A.D., Poudel S., Wu J., Miller D.J., Lin W., Yang L., Bwayi M.N., Rimmer M.A., Gee R.R., Seetharaman J., Chai S.C, & Chen T. (2023). A bromodomain-independent mechanism of gene regulation by the BET inhibitor JQ1: direct activation of nuclear receptor PXR. Nucleic Acids Research, 52(4), 1661-1676.

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High-Throughput Transfection and Luciferase Assay

2022

Lipofectamine 3000, penicillin–streptomycin, and puromycin stock solutions; Opti-MEM Reduced Serum Medium; phenol red–free Dulbecco's modified Eagle's medium; and DMSO were all purchased from Thermo Fisher Scientific (Atlanta, GA), and Fugene 6 was purchased from Promega (Madison, WI). Characterized fetal bovine serum (FBS) and charcoal/dextran treated FBS were purchased from HyClone (Logan, UT). Dual-Glo Luciferase Reporter Assay reagent was purchased from Promega (Madison, WI). The 96-well white tissue culture–treated plates and steadylite HTS reagent were purchased from PerkinElmer Life and Analytical Sciences (Boston, MA). Rifampicin (RIF) and CITCO were purchased from Tocris Bioscience (Minneapolis, MN). SPA70 was synthesized by WuXi App Tec (Wuhan, China). All tissue culture supplemental materials, such as tissue culture flasks and disposable pipettes, were purchased from Corning (Corning, NY).

Bwayi M.N., Garcia-Maldonado E., Chai S.C., Xie B., Chodankar S., Huber A.D., Wu J., Annu K., Wright W.C., Lee H.M., Seetharaman J., Wang J., Buchman C.D., Peng J, & Chen T. (2022). Molecular basis of crosstalk in nuclear receptors: heterodimerization between PXR and CAR and the implication in gene regulation. Nucleic Acids Research, 50(6), 3254-3275.

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Chemical Compound Acquisition for Experimental Research

2020

DMSO, rifampicin, T0901317, SR12813, 17α-ethinylestradiol (EE2), and trans-nonachlor were purchased from Sigma-Aldrich. SPA70, SJB7, and SJC2 were obtained from WuXi AppTec [16 (link)]. All oligonucleotides were from Integrated DNA Technologies and are presented in Supplementary Table 2.

Huber A.D., Wright W.C., Lin W., Majumder K., Low J.A., Wu J., Buchman C.D., Pintel D.J, & Chen T. (2020). Mutation of a single amino acid of pregnane X receptor switches an antagonist to agonist by altering AF-2 helix positioning. Cellular and molecular life sciences : CMLS, 78(1), 317-335.

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