24 well plate
24-well plates are a type of laboratory equipment designed for cell culture and other biological assays. They provide a standardized and consistent platform for conducting experiments with multiple samples simultaneously. The plates feature 24 individual wells, each capable of containing a small volume of liquid or sample. This allows researchers to perform multiple tests or experiments in parallel, improving efficiency and reproducibility.
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12 protocols using «24 well plate»
Cas13-Mediated RNA Manipulation in 293T Cells
Quantifying HAV Infection in Huh7 Cells
Luciferase Assay for Antiviral Screening
Multispecies Biofilm Formation Protocol
C. albicans was seeded in Anaero Columbia blood agar medium (Becton Dickinson, Tokyo, Japan) and incubated under aerobic conditions at 37 °C for 48–72 h. The colonies were diluted with SCD-YE medium as described earlier and incubated for 24 h at 37 °C under aerobic conditions, after which an SCD-YE medium was added to prepare a suspension with an optical density (OD) of 0.05. Then, S. mutans was seeded in an Anaero Columbia blood agar medium (Becton Dickinson, Tokyo, Japan) and incubated under anaerobic conditions at 37 °C for 48–72 h. Each colony was diluted in an SCD-YE medium and incubated under anaerobic conditions for 24 h at 37 °C. After incubation, the SCD-YE medium was added to prepare a bacterial suspension with an OD of 0.5.
The biofilm was formed by treating the PMMA substrate with a mixture of the C. albicans and S. mutans suspensions, as previously reported38 (link),39 (link). In addition, sodium chloride was added to the medium until it reached a concentration of 40 mg/L40 (link). Specifically, the SCD-YE-GS medium, C. albicans suspension, S. mutans suspension, and sterile filtered 4.85% calcium chloride solution were mixed in a ratio of 1367:30:100:3 (v/v/v/v), respectively. The PMMA substrate was placed in each well of a 24-well plate (AGC Techno Glass Co., Ltd., Shizuoka, Japan), and 1 mL of the mixed medium was added, which was then incubated under anaerobic conditions for 16 h at 37 °C to yield a model biofilm substrate.
Canine Keratinocyte Cell Line Analysis
Co., Ltd., Shizuoka, Japan); after washing with PBS to remove all sera, then the cells were serum-starved for 24 hr. Total RNA was extracted from cultured cells in triplicate using the RNeasy Plus Micro Kit (Qiagen).
Extracted total RNA was used in RT-PCR employing the SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen Life Technologies, Carlsbad, CA, U.S.A.).
Real-time reverse transcription (RT)-PCR was performed on samples extracted from Sqc-1 and CPEK. Total RNA was extracted from Sqc-1 and CPEK using the RNeasy Plus Micro Kit. Extracted total RNA was used in RT-PCR
employing the SuperScript® VILO™ cDNA Synthesis Kit. Quantification of TGF-β1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was performed using StepOne™ Real-Time PCR Systems (Applied Biosystems,
Foster City, CA, U.S.A.) and TaqMan Universal PCR Master Mix (Applied Biosystems), with sample cDNA in a final volume of 20 µl per reaction. Canine TGF-β1 mRNA primers and probes were designed by Applied
Biosystems and supplied as Taqman Gene Expression Assays Mix containing a 20× mix of unlabeled PCR forward and reverse primers, as well as a Taqman MGB probe (Assay ID: Cf02623325_m1). The PCR reaction of periostin mRNA
was performed in duplicate for each sample and mean values of the gene expression were calculated as a ratio to those of GAPDH according to the ΔΔCT method.
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