Free access supported by contributions and sponsoring — share your knowledge or support us financially
Search / Compare / Validate Lab equipment & Methods

24 well plate

Manufactured by AGC Techno Glass
Sourced in Japan
About the product

24-well plates are a type of laboratory equipment designed for cell culture and other biological assays. They provide a standardized and consistent platform for conducting experiments with multiple samples simultaneously. The plates feature 24 individual wells, each capable of containing a small volume of liquid or sample. This allows researchers to perform multiple tests or experiments in parallel, improving efficiency and reproducibility.

Automatically generated - may contain errors

Market Availability & Pricing

Is this product still available?

Get pricing insights and sourcing options

Product FAQ

12 protocols using «24 well plate»

1

Cas13-Mediated RNA Manipulation in 293T Cells

2024
The 293T and 293T/BoDV cells were grown in 24-well plates (AGC techno glass, Shizuoka, Japan) for 24 h before transfection at 37 °C and 5% CO2. The cells were transfected with 210 ng of the Cas13-expressing plasmid and 280 ng of the indicated crRNA-expressing plasmids using polyethylenimine “Max” (1 mg/mL) (Polysciences, Warrington, PA, USA). Transfected cells were incubated for 2 days to evaluate the RNA levels.
+ Open protocol
+ Expand Check if the same lab product or an alternative is used in the 5 most similar protocols
2

Quantifying HAV Infection in Huh7 Cells

2023
Huh7 cells were seeded 24 h prior to infection at a density of 5 × 104 cells/well on cover slips in a 24-well plate (AGC Techno Glass). The cells were washed twice with PBS (Fujifilm Wako) and infected with the HAV HM175-18f genotype IB strain at a multiplicity of infection (MOI) of 0.1 in serum-free RPMI. Then, 10 μM masitinib and 0.1 μg/mL interferon-α-2a (Sigma-Aldrich) were added in HAV-infected Huh7 cells. After 24 h of incubation, the cells were washed once with PBS, followed by the addition of 500 µL of RPMI containing 5% FBS, with 10 μM masitinib and 0.1 μg/mL of interferon-α-2a. After 72 h of infection, cells were fixed with ice-cold methanol for 5 min at −20 °C. After the removal of methanol, cells were dried for 3 min, then washed with PBS twice. Later, the cells were blocked using a blocking solution (3% BSA in PBS) for 30 min at room temperature, and the cells were incubated for 1 h with a primary antibody against HAV VP1 (anti-HAV VP1 Antibody (aa7-143), 1:50, LS-C137674, LifeSpan BioSciences, Seattle, WA, USA), followed by a secondary antibody Alexa Fluor 488 F(ab’)2 fragment of goat anti-mouse IgG (1:500, A-11017, Thermo Fisher Scientific). Nuclei were stained with 5 μg/mL Hoechst 33342 (Sigma-Aldrich) in PBS for 10 min, and the cover slip was mounted using an anti-fluorescent quencher (SlowFad Gold Antifade Mountant, Thermo Fisher Scientific). Immunofluorescence images were captured under a Keyence BZ-X710 fluorescence microscope (Takatsuki, Osaka, Japan) using 40× objective magnification.
+ Open protocol
+ Expand Check if the same lab product or an alternative is used in the 5 most similar protocols
3

Luciferase Assay for Antiviral Screening

2022
Twenty-four hours prior to transfection, approximately 1 × 105 cells/well were seeded on a 24-well plate (AGC TECHNO GLASS, Tokyo, Japan). Cells were transfected with 0.2 μg pT7-18f-LUC (HAV strain HM175 18f) using Effectene Transfection Reagent (Qiagen) following the manufacturer’s protocol. After 24 h of transfection, the cells were treated with 0, 10, and 100 μg/mL of the five compounds. After 72 h of transfection, cells were harvested using reporter lysis buffer (Toyo Ink, Tokyo, Japan), and luciferase activity was determined using Luminescencer JNR II AB-2300 (ATTO, Tokyo, Japan).
+ Open protocol
+ Expand Check if the same lab product or an alternative is used in the 5 most similar protocols
4

Multispecies Biofilm Formation Protocol

2021
The following strains were obtained from the Gene Engineering Division of the RIKEN BioResource Research Center (Tsukuba, Japan): Candida albicans JCM1542 (hereinafter, C. albicans) and Streptococcus mutans JCM5705 (hereinafter, S. mutans). A liquid medium containing 3% DAIGO soybean-casein digest broth and 0.5% Bacto yeast extract (hereafter, SCD-YE medium) and a medium containing 1% D( +)-glucose and 2% sucrose added to the SCD-YE medium (hereinafter, the SCD-YE-GS medium) were prepared.
C. albicans was seeded in Anaero Columbia blood agar medium (Becton Dickinson, Tokyo, Japan) and incubated under aerobic conditions at 37 °C for 48–72 h. The colonies were diluted with SCD-YE medium as described earlier and incubated for 24 h at 37 °C under aerobic conditions, after which an SCD-YE medium was added to prepare a suspension with an optical density (OD) of 0.05. Then, S. mutans was seeded in an Anaero Columbia blood agar medium (Becton Dickinson, Tokyo, Japan) and incubated under anaerobic conditions at 37 °C for 48–72 h. Each colony was diluted in an SCD-YE medium and incubated under anaerobic conditions for 24 h at 37 °C. After incubation, the SCD-YE medium was added to prepare a bacterial suspension with an OD of 0.5.
The biofilm was formed by treating the PMMA substrate with a mixture of the C. albicans and S. mutans suspensions, as previously reported38 (link),39 (link). In addition, sodium chloride was added to the medium until it reached a concentration of 40 mg/L40 (link). Specifically, the SCD-YE-GS medium, C. albicans suspension, S. mutans suspension, and sterile filtered 4.85% calcium chloride solution were mixed in a ratio of 1367:30:100:3 (v/v/v/v), respectively. The PMMA substrate was placed in each well of a 24-well plate (AGC Techno Glass Co., Ltd., Shizuoka, Japan), and 1 mL of the mixed medium was added, which was then incubated under anaerobic conditions for 16 h at 37 °C to yield a model biofilm substrate.
+ Open protocol
+ Expand Check if the same lab product or an alternative is used in the 5 most similar protocols
5

Canine Keratinocyte Cell Line Analysis

2018
Commercially available canine keratinocyte cell line (CPEK, CELLnTEC Advanced Cell Systems, Bern, Switzerland) was also used as control in the present study. Sqc-1 or CPEK were grown in 24-well plates (AGC Techno Glass
Co., Ltd., Shizuoka, Japan); after washing with PBS to remove all sera, then the cells were serum-starved for 24 hr. Total RNA was extracted from cultured cells in triplicate using the RNeasy Plus Micro Kit (Qiagen).
Extracted total RNA was used in RT-PCR employing the SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen Life Technologies, Carlsbad, CA, U.S.A.).
Real-time reverse transcription (RT)-PCR was performed on samples extracted from Sqc-1 and CPEK. Total RNA was extracted from Sqc-1 and CPEK using the RNeasy Plus Micro Kit. Extracted total RNA was used in RT-PCR
employing the SuperScript® VILO™ cDNA Synthesis Kit. Quantification of TGF-β1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was performed using StepOne™ Real-Time PCR Systems (Applied Biosystems,
Foster City, CA, U.S.A.) and TaqMan Universal PCR Master Mix (Applied Biosystems), with sample cDNA in a final volume of 20 µl per reaction. Canine TGF-β1 mRNA primers and probes were designed by Applied
Biosystems and supplied as Taqman Gene Expression Assays Mix containing a 20× mix of unlabeled PCR forward and reverse primers, as well as a Taqman MGB probe (Assay ID: Cf02623325_m1). The PCR reaction of periostin mRNA
was performed in duplicate for each sample and mean values of the gene expression were calculated as a ratio to those of GAPDH according to the ΔΔCT method.
+ Open protocol
+ Expand Check if the same lab product or an alternative is used in the 5 most similar protocols

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!

🧪 Need help with an experiment or choosing lab equipment?
I search the PubCompare platform for you—tapping into 40+ million protocols to bring you relevant answers from scientific literature and vendor data.
1. Find protocols
2. Find best products for an experiment
3. Validate product use from papers
4. Check Product Compatibility
5. Ask a technical question
Want to copy this response? Upgrade to Premium to unlock copy/paste and export options.