4 6 diamidino 2 phenylindole (dapi)
DAPI (4',6-diamidino-2-phenylindole) is a fluorescent stain used for labeling and visualizing nucleic acids, particularly DNA, in biological samples. It binds strongly to adenine-thymine (A-T) rich regions of the DNA double helix. DAPI can be used in a variety of microscopy techniques to stain the cell nuclei and facilitate the analysis of cellular and subcellular structures.
Lab products found in correlation
26 protocols using 4 6 diamidino 2 phenylindole (dapi)
Multiplex Immunofluorescence for Immune Cell Profiling
Multiplex Immunofluorescence Imaging of Tumor Microenvironment
Histological Evaluation of Liver Tissue
Sections were also stained with anti-CD3 (BioCare medical, Pacheco, CA), CD4 (BioCare Medical), DAPI (Akoya Biosciences, Marlborough, MA) and FOXP3 (BioCare medical) (
Histological and Immunohistochemical Analysis of Small Intestine
Quantifying Neutrophil Infiltration in Mouse Ears
Multispectral Imaging of FFPE Samples
Multiplexed Immunohistochemistry for Tissue Analysis
Multiplex Immunohistochemistry Staining Protocol
Multiplex Immunofluorescence for NSCLC Immune Profiling
The procedure for mIF has been previously described (21 (link), 30 (link)–33 (link)). In summary, 3 μm serial sections obtained from TMA blocks were deparaffinized and rehydrated using a decreasing ethanol series. Antigen dretrieval was performed by using microwaves. A blocking buffer was then used to initiate protein stabilization and background reduction for 30-60 minutes at room temperature. Primary antibodies were applied and washed in 1×Tris-buffered saline containing 0.5% Tween 20. Isotype-specific HRP-conjugated antibodies and an Opal fluorophore (Akoya Biosciences). Finally, the nuclei were stained with DAPI (Akoya Biosciences) for 10 min. Antibodies and fluorophores used in the mIF procedures were detailed in
Histological analysis of small intestine
For CD68 IHC, SIs were fixed in 4% neutral-buffered formalin for 24 h and then stored in PBS at 4 °C until paraffin embedding. Sections (7 μm) were deparaffinized, and staining was performed using a Leica Bond MAX immunostainer (Leica Microsystems, Wetzlar, Germany) and CD68 antibody (1:1,000; Cell Signaling Technologies, Danvers, MA). To visualize neutral lipids and nuclei, cryosections (7 μm) were stained with ORO (Sigma-Aldrich, St. Louis, MO) and DAPI (Akoya Bioscience, Marlborough, MA), respectively. Images were acquired at 60 × magnification (Apo 60× Oil λS objective) using a Nikon Eclipse Ti microscope (NIKON Corporation, Tokyo, Japan) equipped with a Nikon A1 camera.
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