Anti β actin

Name: Anti β actin
Brand: Abcam
SKU: 2SLhCZIBPBHhf-iFeO8t
Availability: InStock

Manufactured by Abcam

2 011 citations

Sourced in United States, United Kingdom, China, Canada, Germany, Hong Kong, Japan, Spain, France, Israel

About the product

Anti-β-actin is a primary antibody used to detect and quantify the β-actin protein in various cell and tissue samples. β-actin is a widely expressed housekeeping gene and its protein product is commonly used as a loading control in Western blot analysis.

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Market Availability & Pricing

Abcam's anti-beta actin antibodies are commercially available through authorized distributors. Prices for these products vary depending on the specific product and distributor. The Anti-beta Actin antibody [8F10-G10] (catalog number AB170325100UL) is listed at $612.00 per 100 µL on Fisher Scientific's website, while the Anti-beta Actin antibody (catalog number ab8227) is priced at $439.00 for 50 µg on Biocompare. Please note that the pricing information provided is from secondary markets and may not reflect the most current or accurate pricing from the manufacturer.

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Pvdf membrane, by Merck Group (188 mentions) Anti bax, by Abcam (74 mentions) Bca protein assay kit, by Beyotime (72 mentions) Anti gapdh, by Abcam (70 mentions) Anti bcl 2, by Abcam (69 mentions) Ripa lysis buffer, by Beyotime (63 mentions) Anti e cadherin, by Abcam (59 mentions) Anti flag, by Merck Group (53 mentions) Anti cleaved caspase 3, by Abcam (47 mentions) Bca protein assay kit, by Thermo Fisher Scientific (46 mentions) Anti vimentin, by Abcam (45 mentions) Anti akt, by Cell Signaling Technology (42 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (42 mentions) Ripa buffer, by Beyotime (42 mentions) Polyvinylidene difluoride membrane, by Merck Group (38 mentions) Anti β catenin, by Abcam (37 mentions) Anti n cadherin, by Abcam (36 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (35 mentions) Polyvinylidene fluoride membrane, by Merck Group (35 mentions) Protease inhibitor cocktail, by Roche (34 mentions)

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2 011 protocols using «anti β actin»

Ginkgolide B Signaling Pathway

2025

Ginkgolide B (GGB) and Dimethyl sulfoxide (DMSO), and corn oil were obtained from Sigma-Aldrich. The antibodies used were: anti-GSDMD, anti-Bax, anti-Bcl-2, anti-β-actin, anti-p-Akt, anti-Akt, anti-p-mTOR, anti-mTOR, anti-E-cadherin, and anti-N-cadherin (1:1000 dilution), and HRP-labelled goat anti-mouse IgGs (1:2000 dilution), all from Abcam.

Lu X., Zhang Y., Wang R, & Li Z. (2025). Ginkgolide B Inhibits EMT and Promotes Pyroptosis in Gastric Cancer via AKT/mTOR Pathway. Drug Design, Development and Therapy, 19, 2491-2502.

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CIC Mutation Effects on Epithelial-Mesenchymal Transition

2025

Western blot analysis and immunofluorescence stain were performed using 1) #6507A tumor cells derived from one tissue of a Sub5 patient carrying a frame shift deletion mutation in the C-terminal region of CIC and 2) #6595 tumor cells (control) derived from one tissue of a Sub3 patient as a control without CIC mutation. Each experiment was independently repeated twice. Cytoplasmic, membrane and nuclear protein extracts were prepared with a subcellular protein fraction Kit (Thermo Scientific, 78,840) and used for western blot analysis. Western blotting was carried out according to the standard procedures using the enhanced-chemiluminescence detection (Amersham™, RPN2232). The following antibodies were used: anti-CIC (Invitrogen, PA5-83,721), anti-ETV4 (Proteintech, 10,684–1-AP), anti-CDH1 (Cell Signaling Technology, Clone: 24E10), anti-CTNNA1 (BD Bioscience, Clone: 5), anti-CDH2 (BD Bioscience, Clone: 32), anti-VIM (BD Bioscience, Clone: RV202), anti-β-Actin (Abcam, Clone: AC-15). Immunofluorescence stain was performed with patient-derived tumor cells. The cells were attached onto glass slides for O/N and fixed in 3.7% formaldehyde at 4℃ cold room for 30 min. They were permeabilized with 0.5% Triton X-100 in PBS (2 mM MgCl₂) at room temperature for 10 min and treated with 5% BSA in PBS (2 mM MgCl₂) for 1 h to block nonspecific reaction. Sequential incubation of primary antibody and Alexa fluorescence-conjugated secondary antibody (Invitrogen, A11037 and A11029) was performed using DAPI (Invitrogen, D1306) as a counterstain. Immunofluorescence stain images were acquired at 40 × magnification using a Zeiss LSM 780 confocal microscope.

Hyeon D.Y., Nam D., Shin H.J., Jeong J., Jung E., Cho S.Y., Shin D.H., Ku J.L., Baek H.J., Yoo C.W., Hong E.K., Lim M.C., Lee S.J., Bae Y.K., Kim J.K., Bae J., Choi W., Kim S.J., Back S., Kang C., Madar I.H., Kim H., Kim S., Kim D.K., Kang J., Park G.W., Park K.S., Shin Y., Kim S.S., Jung K., Hwang D., Lee S.W, & Kim J.Y. (2025). Proteogenomic characterization of molecular and cellular targets for treatment-resistant subtypes in locally advanced cervical cancers. Molecular Cancer, 24, 77.

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HBx Protein Expression and Stability

2025

The DNA fragment encoding C-terminal HA tagged HBx was amplified from the cDNA of wildtypes A1 and A2, and mutant A1 using primers 5’-CTAGCTAGCTAGATGGCTGCTAGG-3’ and 5’-CCGCTCGAGCGGTCCGGCAGAGGTGAA-3’ with NheI and XhoI sites underlined. The amplified HBX fragments were cloned into NheI/XhoI sites of vector pCMV6-AC-3HA with C-terminal 3XHA tag (OriGene, MD, USA). HepG2 cells were transfected with an empty vector (pHA) or various HBx constructs (pHBx-HA). Two days later, cells were treated with or without MG132 proteasome inhibitor (Sigma, MO, USA) at 10 μM for 6 hours. Cells were then lysed with protein lysis RIPA buffer (Thermo Fisher Scientific, MA, USA) containing protease inhibitor cocktail (Sigma, MO, USA) according to manufacturer’s protocol. Proteins were then quantified with Pierce BCA assay kit (Thermo Fisher Scientific, MA, USA). All samples were loaded with the same amounts of proteins at 750 μg/ml. Western blots were performed with Protein Simple WES Western Blot System (Bio-Techne) and followed manufacturer’s protocol. The ProteinSimple 12–230 kDa separation module (Thermo Fisher Scientific, MA, USA) was used. The primary antibodies anti-HA (Thermo Fisher Scientific, MA, USA) and anti-β-actin (Abcam, Cambridge, UK) were used to detect HBx-3xHA and β-actin (used as loading control) respectively. The secondary antibodies anti-mouse and anti-rabbit detection modules (Thermo Fisher Scientific, MA, USA) were used. Analyses of western blot were performed on the Compass for Simple Western software.

Zhang M., Mouzannar K., Zhang Z., Teraoka Y., Piotrowski J., Ishida Y., Tateno-Mukaidani C., Saito T., Abe-Chayama H., Chayama K, & Liang T.J. (2025). Hepatitis B virus genotypes A1 and A2 have distinct replication phenotypes due to polymorphisms in the HBx gene. PLOS Pathogens, 21(1), e1012803.

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Protein Extraction and Detection from Glioma Cells

2025

Total proteins were extracted from gliomas cells using RIPA lysis buffer (Beyotime, Shanghai, China). Post-extraction, membranes were washed six times with TBST for five minutes each and incubated overnight at 4 °C with HRP-conjugated secondary antibodies at a 1:2000 dilution. Protein levels were detected using the ChemiDOC™ XRS + system (Bio-Rad). The primary antibodies used included anti-CDKN3(Santa Cruz, sc-135864), anti-ARG1 (Beyotime, AF1381), anti-beta-actin (Abcam, ab8226), Goat Anti-Rabbit IgG H&L (HRP) (Sangon Biotech, D110058), and Goat Anti-Mouse IgG H&L (HRP) (CST, 91196).

Cheng H., Meng X., Zhang Y., Wang P, & Lu Y. (2025). Identification of CDKN3 overexpression as a marker of poor prognosis and potential therapeutic target in low-grade glioma. Scientific Reports, 15, 2679.

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Breast and Prostate Cancer Cell Culture

2025

DMEM/F12 (cat. No. G4610-500ML), DMEM (cat. No. G4511-500ML), L-glutamine (cat. No. G4211-100ML), HCl, Earle’s salts solution (cat. No. G4215-500ML), Hank’s salts solution (cat. No. G4204-500ML), Versene’s solution (cat. No. G4050-100ML), antibiotic/antimycotic mixture (penicillin, streptomycin, amphotericin B, cat. No. G4015-100ML), and trypsin (cat. No. G4012-100ML) were from Servicebio, Wuhan, China. Fetal bovine serum (cat. No. FBS-11A) was from Capricorn Scientific, Wuhan, China.
The cell lines MDA-MB-231 (HTB-26), MCF-10A (CRL-10317), MCF-7 (HTB-22), BT-474 (HTB-20), BT-20 (HTB-19), SK-BR-3 (HTB-30), and DU 145 (HTB-81) were purchased from ATCC, Manassas, VA, USA.
Antibodies anti-b-actin and anti-CB2 were from Abcam, Cambridge, UK. Anti-mouse IgG antibody was from Jackson ImmunoResearch, Cambridge, UK.
SR 144028, PSB CB5, ML-184, ML-193, capsazepine, and LPI was from Tocris Bioscience, Bristol, UK. DMSO, resazurin, D-glucose, glycylglycine, acetic acid, MgSO_4, EGTA, dithiothreitol, acrylamide, bis-acrylamide, Triton X-100, SDS, nitro blue tetrazolium, Tris, EDTA, agarose, bicinchoninic acid, bovine serum albumin, anti-rabbit IgG antibody, diclofenac, N-acetyl cysteine, and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St. Louis, MO, USA. The purity of all the used reagents was 95% or more.

Akimov M.G., Gretskaya N.M., Gorbacheva E.I., Khadour N., Sherstyanykh G.D, & Bezuglov V.V. (2025). Two-Step Cell Death Induction by the New 2-Arachidonoyl Glycerol Analog and Its Modulation by Lysophosphatidylinositol in Human Breast Cancer Cells. International Journal of Molecular Sciences, 26(2), 820.

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Top 5 most cited protocols using «anti β actin»

Transcriptional Analysis of T Cell Subsets

Cited 20 times

Total RNA was isolated from transduced (Ametrine⁺) CD4⁺ or CD8⁺ T cells and used for cDNA synthesis as previously described (Johnston et al., 2009 (link)). qPCR reactions were performed in triplicate using the SYBR Select Master Mix (Life Technologies) on a Roche Lightcycler 480, using primers specific to Prdm1 (F-5’-TTCTCTTGGAAAAACGTGTGGG-3’; R-5’-GGAGCCGGAGCTAGACTTG-3’) and Tbx21 (F-5’ -ACCAACAACAAGGGGGCTTC-3’; R-5’ -CTCTGGCTCTCCATCATTCACC −3’). For western blot analysis, whole cell lysates were obtained from CD8⁺ T cells on day 6 after activation, CD4⁺ T cells 5 days after activation, or from MCC-T cells by sorting transduced (Ametrine⁺) cells and lysis in 150 mM NaCl, 25 mM Tris pH 7.5, 1 % Triton X-100, 0.1% SDS, 0.5% Deoxycholate, and complete protease inhibitors (Roche). 25 µg of protein was resolved by 8% SDS PAGE, transferred to nitrocellulose membranes and probed with anti-Cyclin T1 (sc-10750), anti-Blimp1 (sc-47732), anti-Cdk9 (sc-484) (Santa Cruz Biotechnology), anti-Perforin (ab16074) and anti-beta Actin (Abcam ab8227).

Chen R., Bélanger S., Frederick M.A., Li B., Johnston R.J., Xiao N., Liu Y.C., Sharma S., Peters B., Rao A., Crotty S, & Pipkin M.E. (2014). In vivo RNA interference screens identify regulators of antiviral CD4+ and CD8+ T cell differentiation. Immunity, 41(2), 325-338.

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Corresponding organizations : Scripps Research Institute, La Jolla Institute For Allergy & Immunology

Immortalized Human Mammary Epithelial Cells

Cited 16 times

Immortalized human mammary epithelial cells (HMLE) and V12H-Ras transformed derivatives (HMLER), including cells expressing empty vector (pWZL), Snail, Twist, Goosecoid (GSC) or an activated form of TGF-β1 were maintained as previously described (8 (link)). Established human breast cancer cell lines were cultured in cell specific medium as outlined in the SI Materials and Methods. Antibodies used included; anti-β-actin (Abcam), FOXC2 (Dr. Naoyuki Miura), E-cadherin (BD Bioscience), Fibronectin (BD Bioscience), N-cadherin (BD Bioscience), Vimentin (NeoMarkers) and β-catenin (BD Bioscience).

Hollier B.G., Tinnirello A.A., Werden S.J., Evans K.W., Taube J.H., Sarkar T.R., Sphyris N., Shariati M., Kumar S.V., Battula V.L., Herschkowitz J.I., Guerra R., Chang J.T., Miura N., Rosen J.M, & Mani S.A. (2013). FOXC2 expression links epithelial-mesenchymal transition and stem cell properties in breast cancer. Cancer research, 73(6), 1981-1992.

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Corresponding organizations : Baylor College of Medicine, Hamamatsu University School of Medicine, Rice University, The University of Texas Health Science Center at Houston, The University of Texas MD Anderson Cancer Center

Detailed Antibody Characterization in Alzheimer's

Cited 11 times

For the methods used in this study, the following primary antibodies were utilized: MOAB-2 (anti-Aβ, mouse IgG_2b, 0.5 mg/ml), IgG_2b(0.2 mg/ml, Sigma-Aldrich, St. Louis, MO), 6E10 anti-Aβ residues 3-8, mouse IgG₁, 0.5 mg/ml; Covance, Princeton, NJ), 22C11 (anti-APP N-terminal, mouse IgG₁, 1 mg/ml, Milipore, Billerica, MA), 4G8 anti-Aβ residues 17-24, mouse IgG, Senetek, Maryland Height, MD), CT1565 (anti-APP C-terminal, rabbit monoclonal #1565, 0.45 mg/ml, Epitomics, Burlingame, CA), CT695 (anti-APP C-terminal, rabbit polyclonal #51-2700, 0.25 mg/ml, Invitrogen, Carlsbad, CA), anti-Aβ40 (MM32-13.1.1, mouse IgG₁, 1.8 mg/ml, Bu lab), anti-Aβ42 (rabbit, 0.35 mg/ml; Invitrogen, Carlsbad, CA), anti-β-actin (chicken, 1.0 mg/ml; Abcam, Cambridge MA) cathepsin-D (goat polyclonal, 0.2 mg/ml, Santa Cruz biotechnology, Santa Cruz, CA). The dilutions of each antibody stock are denoted in the appropriate Methods section or Figure Legend.

Youmans K.L., Tai L.M., Kanekiyo T., Stine WB J.r., Michon S.C., Nwabuisi-Heath E., Manelli A.M., Fu Y., Riordan S., Eimer W.A., Binder L., Bu G., Yu C., Hartley D.M, & LaDu M.J. (2012). Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody. Molecular Neurodegeneration, 7, 8.

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Corresponding organizations : University of Illinois Chicago, Mayo Clinic in Florida, NorthShore University HealthSystem, Rush University Medical Center, Northwestern University

Antibodies and qRT-PCR Assay Protocol

Cited 10 times

The following antibodies were used: rabbit anti-PHD1 (P. Ratcliffe), rabbit anti-PHD2 (P. Maxwell; Novus), rabbit anti-PHD3 (Novus), rabbit anti-HIF-1, rabbit anti-HIF-2 (Novus), goat anti-VE-cadherin (R&D Systems), and rabbit anti-β-tubulin or anti-β-actin (Abcam). sFlt1 was measured by immunoassays (R&D Systems). Quantitative RT-PCR was performed as described (Fischer et al., 2007 (link)). For Assay ID (Applied Biosystems) and sequence of primers and probes, see Table S2.

Mazzone M., Dettori D., de Oliveira R.L., Loges S., Schmidt T., Jonckx B., Tian Y.M., Lanahan A.A., Pollard P., de Almodovar C.R., De Smet F., Vinckier S., Aragonés J., Debackere K., Luttun A., Wyns S., Jordan B., Pisacane A., Gallez B., Lampugnani M.G., Dejana E., Simons M., Ratcliffe P., Maxwell P, & Carmeliet P. (2009). Heterozygous Deficiency of PHD2 Restores Tumor Oxygenation and Inhibits Metastasis via Endothelial Normalization. Cell, 136(5), 839-851.

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Corresponding organizations : KU Leuven, Vlaams Instituut voor Biotechnologie, University of Turin, Candiolo Cancer Institute, Wellcome Centre for Human Genetics, Yale University, FIRC Institute of Molecular Oncology, University College London

Western Blot Analysis of TGF-β Signaling

Cited 6 times

Retinae were lysed for 15 minutes on ice in RIPA buffer (1% SDS, 1% sodium deoxycholate, 0.1% Nonidet P-40 in 50 mM Tris-HCl (pH 7.6)/150 mM NaCl) plus protease (Complete inhibitor tablet, Roche) and phosphatase (5 mM NaF and 1 mM orthovanadate) inhibitors. Cell lysates were cleared and protein concentrations determined via Bradford analysis. Cell free extract (25 μg) was loaded per lane on a 12% (Smad/phospho-Smad) or 15% (TGFβII) SDS-PAGE gel. Protein was then transferred to PVDF membrane at 80 V for 1 h. Membranes were blocked in 5% skim milk powder or 5% bovine serum albumin (for phospho-Smad) in tris-buffered saline with 0.1% tween 20 (TBST) and then incubated with anti-phospho-Smad2/3 (1/200; Santa Cruz), Smad2/3 (1/200; Santa Cruz), TGFβII (1/200; Santa Cruz) or anti-β-actin (1/5,000, AbCam [Cambridge, MA, USA]) overnight at 4°C. Membranes were washed three times for ten minutes each prior to incubation in horse radish peroxidase (HRP)-conjugated secondary antibodies and development with ECL (Roche).

Ma L., Cantrup R., Varrault A., Colak D., Klenin N., Götz M., McFarlane S., Journot L, & Schuurmans C. (2007). Zac1 functions through TGFβII to negatively regulate cell number in the developing retina. Neural Development, 2, 11.

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Corresponding organizations : University of Calgary, Institut de Génomique Fonctionnelle

Spelling variants (same manufacturer)

β-actin - 4713 citations Mouse anti-β-actin - 408 citations Anti-actin - 374 citations Rabbit anti-human β-actin antibody - 14 citations Mouse anti-human β-actin antibody - 14 citations Anti-β-actin polyclonal antibody - 9 citations Anti‐β‐actin antibody (ab8226; - 6 citations Anti-β- - 5 citations Anti-β-actin (AC-15) (ab6276), - 3 citations Rabbit anti-β-actin primary antibody - 3 citations

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