The largest database of trusted experimental protocols

Anthos Zenyth 3100

Manufactured by Anthos Labtec
Sourced in Austria, United States

The Anthos Zenyth 3100 is a compact and versatile laboratory instrument designed for spectrophotometric analysis. It features a monochromator-based optical system that allows for accurate wavelength selection and measurement of absorbance, transmittance, or reflectance of samples across a wide range of the electromagnetic spectrum.

Automatically generated - may contain errors

9 protocols using Anthos Zenyth 3100

Ceramic
granules (0.2 cc, n = 6) were soaked into 2 mL of
medium composed of minimal essential medium-alpha (a-MEM; Gibco, Invitrogen,
U.K.) supplemented with 10% fetal bovine serum (FBS, Lonza, Germany)
and 1% penicillin/streptomycin (Gibco, Invitrogen, U.K.), and incubated
at 37 °C in a humid atmosphere with 5% CO2 for 4 days.
After washing three times with phosphate buffer solution (PBS, Invitrogen,
Darmstadt, Germany), 0.5 mL of 1% Triton solution was added to each
sample. Thereafter the amount of protein detached from the samples
into the Triton solution was measured with the QuantiPro BCA Assay
Kit (Pierce Biotechnology Inc., Rockford, U.S.A.) following the manufacturer’s
instructions, and absorbance was measured with a spectrophotometer
(Anthos Zenyth 3100, Anthos Labtec Instruments GmbH, Salzburg, Austria)
at 620 nm. A calibration curve was prepared using standard FBS solutions.
The amount of proteins adsorbed onto the ceramics was then converted
to the same amount of the material (1.0 cc) and expressed as mean
± SD.
+ Open protocol
+ Expand
Yeast strains were grown in YPD to mid-exponential phase and were then washed and resuspended in PBS. After exposure to heat shock (42 °C for 2, 4, 6, 8, 10, 12, 14 and 16 min) cells were fixed with 3.7% formaldehyde. In addition, yeast cells harboring either the plasmid pCM666-mBAX (murine BAX under control of a tet-inducible promoter) or pCM666 were incubated for 16 h in the presence of 10 μg/ml doxycycline hyclate at 28 °C in SC-leu medium. All cells were then stained with 1 mg/ml Nile Red (Thermo Fisher Scientific, N-1142) and incubated in the dark for 20 min before measuring relative fluorescence with the multi-plate reader Anthos Zenyth 3100 (Anthos Labtec Instruments GmbH, Wals-Siezenheim, Austria).
+ Open protocol
+ Expand
2.5 × 107 cells were washed two times with 1x PBS. After centrifugation for 3 min at 3,500 rpm the pellet was resolved in 500 µL PBS-DHE (1:1,000 dilution of a 5 mg/ml DHE stock, Sigma Aldrich—37,291). The samples were incubated for 30 min in the dark without shaking. 200 µL of the solution were pipetted into each well and the fluorescence was measured with Anthos Zenyth 3,100 (Anthos Labtec Instruments GmbH, Salzburg, Austria). Excitation was set at 535 nm and emission was detected at 625 nm for 4 s.
+ Open protocol
+ Expand
Cells were cocultured with low or high concentrations of BDNF for 48 h, then 10 μL WST-1 (Roche Applied Science, Basel, Switzerland) was added and incubated for 60 min. The intensity of color formation was detected using Anthos Zenyth 3100 multimode fluorometer (Anthos Labtec Instruments GmbH, Salzburg, Austria).
+ Open protocol
+ Expand
After washing two times with 1x PBS, 1 × 107 cells in a volume of 225 µL were pipetted into a 96-well plate. The cells suspension was mixed with 25 µL formaldehyde (37%). After addition of 1 µL Nile red (0.001 mg/ml in acetone, Thermo Fisher Scientific, N-1142), the plate was incubated for 20 min in the dark on a shaker and the fluorescence was measured using the Anthos Zenyth 3,100 (Anthos Labtec Instruments GmbH, Salzburg, Austria). Excitation was set at 485 nm and emission was detected at 595 nm for 0.4 s. Because elutriation yields were low cell numbers, a modified protocol was used after elutriation: 0.5 × 107 cells and 0.002 mg/ml Nile red in acetone without formaldehyde fixation were used.
+ Open protocol
+ Expand
All DNA samples were extracted from 250 μl of whole blood using a commercially available kit according to the manufacturer's instructions (QIAamp DNA extraction kit; Qiagen, Hilden, Germany). After extraction, the determination of the concentration of all DNA samples was carried out using the PicoGreen dsDNA quantification reagent (Molecular Probes, Eugene, OR, USA) in an Anthos Zenyth 3100 (Anthos-Labtec Instruments GmbH, Austria). The linearity of the method was verified for the high-range standard curve (2 μg/ml of Lambda DNA standard) according to the manufacturer's recommendations prior to determination the DNA concentration.
+ Open protocol
+ Expand
GFP fluorescence measurements were performed using the Anthos Zenyth 3100 (Anthos Labtec Instruments GmbH, Salzburg, Austria) (excitation: 535 nm; emission: 625 nm; detection time: 4 s). To compensate for clonal differences in GFP expression levels, stressed samples in each single case were normalized to the respective unstressed samples.
+ Open protocol
+ Expand
An aliquot of 5 × 106 cells in a volume of 200 μL PBS was pipetted in a black microwell plate (Nunc). The fluorescence was then measured in an Anthos Zenyth 3100 (Anthos Labtec Instruments) plate reader with an excitation wavelength of 485 nm and an emission wavelength of 595 nm.
+ Open protocol
+ Expand
Anti-CCP was detected by Quanta Lite CCP3 enzyme-linked immunosorbent assay (ELISA) kits (Inova Diagnostics, San Diego, CA, USA) in accordance to the manufacturer’s recommendations. ELISA plates (BD Biosciences, San Jose, CA, USA) were coated for 2 h at 37 °C with 1 μg/mL GRP78 or CarGRP78 in coating buffer containing 35 mM NaHCO3 and 15 mM Na2CO3, pH 9.6. The plates were washed with phosphate-buffered saline (PBS) for four times and blocked with PBS containing 2% skim milk overnight. Then, Quanta Lite reagents (Inova Diagnostics, San Diego, CA, USA) were used for performing ELISA according to the supplier’s protocol. The plates were read at 405 nm on an Anthos Zenyth 3100 multimode fluorometer (Anthos Labtec Instruments GmbH, Salzburg, Austria). All samples were analyzed at the same time. The absorbance values of control wells were low in all cases. The cutoff values for the anti-GRP78 and anti-CarGRP78 antibody ELISA was defined as the mean plus two standard deviations of the controls.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!