OneScript Plus cDNA Synthesis Kit
The OneScript Plus cDNA Synthesis Kit is a tool used for the reverse transcription of RNA into complementary DNA (cDNA) for downstream applications. It includes reagents and buffers necessary for the conversion process.
Lab products found in correlation
29 protocols using OneScript Plus cDNA Synthesis Kit
RNA Isolation and cDNA Synthesis
Drosophila Gene Expression Analysis
cdk8 F CATCCGGGTGTTTCTGTCG
cdk8 R CAGCCCGATGGAACTTAATGAT
cycC F AGTTTCCCTACCGCACCAATC
cycC R ACAATCAAGCAGCAATCCAGG
pink1 FAAGCGAGGCTTTCCCCTAC
pink1 R GCACTACATRGACCACCGATTT
parkin F GAAGCCTCCAAGCCTCTAAATG
parkin R ACGGACTCTTTCTTCATCGGT
opa1 F CAAGCTGCGATACATCGTCC
opa1 R GCAGTCCATCCTTCCATTCC
marf F GAGACGACCACCTTTATCAACG
marf RCCACCTTCATGTGATCCCG
drp1 FACAGCCCACTCGATGATCG
drp1 RAAGCACTTCTTGGTGTGCAG
rp49 FAGCATACAGGCCCAAGATCG
rp49 RTGTTGTCGATACCCTTGGGC
Imaginal Disc RNA Extraction and qRT-PCR
Quantitative Real-Time PCR Analysis
Pear Leaf RNA Extraction and qRT-PCR
Quantifying Osteogenic and Antioxidant Genes
Expression levels of osteogenic-related (Runt-related transcription factor 2; Runx2 and ALP) and antioxidant system-related genes (Nrf2 and Nqo1) were determined using qRT-PCR. For this analysis, predesigned FAM™labeled TaqMan assays and TaqMan™ fast advanced master mix (Thermo Fisher, 4444557) were employed. The Step One Plus™ real-time PCR system (Thermo Fisher, USA) was used to run the PCR reactions. The mRNA levels of Runx2 (Rn01512298_m1), ALP (Rn01516028_m1), Nrf2 (Rn00582415-m1), and Nqo1 (Rn00566528_m1) were quantified using the 2 -ΔΔCT method, with betaactin (Rn00667869_m1) serving as the reference gene for normalization.
PBMC DNA and RNA Extraction and cDNA Synthesis
Evaluating YAP1 Response in A549 Cells
hr. Western blot was used to check for YAP1 protein level. qRT-PCR
was used to check for YAP1 downstream genes transcription (CTGF and
CYR61). Total RNA was extracted using IBI Isolate Total Extraction
Reagent Kit (IB47602). Two milligrams of RNA was reverse transcribed
using OneScript Plus cDNA Synthesis Kit (ABM G236) according to the
manufacturer’s protocol. Real-time PCR was performed using
BlasTaq 2X qPCR MasterMix (ABM G892) on a QuantStudio 3 Real-Time
PCR System. All qPCR reactions were performed in triplicates. The
list of primers is included in the
Quantitative RNA Expression Analysis
qPCR was performed on a qTOWER3
G cycler (IST Innuscreen) using the PerfeCTa® Green FastMix® Low ROX (Quantabio). Samples were analyzed in triplicates with 10 ng input cDNA per reaction and 300 nM forward and reverse primers. Data was normalized to the housekeeping gene GAPDH and fold changes calculated using the comparative Ct (ΔΔCt) method. Primer sequences38 (link),39 (link) are listed in
Quantifying Immune Pathway Transcripts
IFIH1_Forward CTGGGACTAACAGCTTCACCTGGTGTTG. IFIH1_Reverse GCATCTGCAATGGCAAACTTCTTGCATG. To measure NF‐κB target expressions, 500 ng of RNA was retro‐transcribed using OneScript Plus cDNA Synthesis Kit (abm, G236) and the following TaqMan assays were used (Thermo Fisher Scientific): IL‐1b, hs00174097_m1; IL‐6, Hs00985639_m1; IL‐8, hs00174103_m1.
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