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Lactate Pro2 LT-1730

Manufactured by Arkray
Sourced in Japan

The Lactate Pro2 LT-1730 is a portable blood lactate analyzer designed for professional use. It provides accurate and reliable measurements of lactate levels in whole blood samples. The device is compact, easy to use, and delivers results quickly.

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18 protocols using Lactate Pro2 LT-1730

Blood samples collected at 3-min intervals in the first incremental cycling test were used to determine LT and OBLA from La. Blood samples collected at the resting, LT, and OBLA states in subsequent cycling tests were measured using a blood lactate analyser (Lactate Pro 2, LT-1730; ARKRAY, Kyoto, Japan). This analyser operates on an enzymatic amperometric detection method [30 (link)]. It interprets the electrical signal produced due to the reaction between lactate in the blood and the enzyme lactate oxidase on the inserted sensor. The voltage signal directly corresponds to the lactate concentration of the samples, allowing measurement of blood lactate concentration.
Blood samples (approximately 0.3 μL) were collected from each participant’s fingertip after each incremental cycling load exercise for 3 min.
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At 30 s after the end of each high-intensity cycling period during the intermittent cycling exercise test, fingertip blood samples (0.3 μl) were collected to measure blood lactate concentrations ([La]b) using the enzymatic-amperometric detection method (Lactate Pro 2LT-1730; Arkray, Kyoto, Japan).
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The concentration of blood lactate was measured before the onset of running and immediately after exhaustion by treadmill running. Exhaustion was determined by the inability of the mice to run on the treadmill more than 10 s despite stimulation. The measurement was performed with lactate meter (Lactate pro 2, LT-1730, ARKRAY) using blood from a tail tip.
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Venous blood samples were collected immediately after exercise, and blood analysis was performed using whole blood and serum. To collect the serum, the venous blood samples were allowed to clot at 24–25 °C for 30 min, centrifuged at 2000× g for 15 min at 4 °C, and transferred to new tubes before being stored at −80 °C. Whole blood was used to measure the concentration of blood glucose (ACCU CHEK Performa Glucometer, Roche, Diagnostics, Penzberg, Germany), lactate (Lactate Pro2, LT-1730, ARKRAY, Kyoto, Japan), and TG (Standard LipidoCare Strips–Lipid Profile, 02LA10G, SD Biosensor, Suwon, Korea). Serum was used in ELISA kits for the analysis of the concentration of glycerol (EGLY-200, BioAssay System, Hayward, CA, USA) and FFAs (K612-100, BioVision, Milpitas, CA, USA).
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Blood was collected from the caudal vein of each rat immediately before and after exhaustion testing. Blood lactic acid levels were measured using a simplified blood lactic acid measuring device (LactatePro™ 2 LT‐1730; Arkray, Kyoto, Japan), and blood glucose levels were measured using a glucose self‐measuring device (One Touch Ultra; Johnson & Johnson, Tokyo, Japan).
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All participants ran a marathon in an official race (Sénart Marathon, France). The start was at 9 am, and for the environmental conditions on 1 May 2019 in Sénart, the temperature was between 11 and 15 °C (between 9 a.m. and 1 p.m.), there was no precipitation, and the humidity in the air averaged 60%. The blood lactate was measured on the finger (Lactate PRO2 LT-1730; ArKray, Japan) right after the runners’ warm up (15 min at easy pace) and at the third minute after they crossed the finish line.
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All subjects performed a cycle ergometry exercise test according to the local ramp protocol. The test consisted of a gradual increase in workload until exhaustion. Hemodynamic, metabolic, and respiratory parameters, including respiratory rate, tidal volume, and respiratory minute volume, were recorded. Measurement of height and weight and spirometry (Jaeger Vyntus CPX, Vyaire Medical, USA) were performed before the exercise test. Before and after exercise, a capillary blood gas sample was taken. If a capillary blood gas sample after exercise could not be obtained, blood lactate was measured using Lactate Pro2 LT-1730 (Arkray, Japan).
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At 1 and 3 min after the end of the maximal graded exercise test and intermittent sprint cycling exercise tests, fingertip blood samples (0.3 μl) were collected to measure blood lactate concentrations ([La]b) using the enzymatic-amperometric detection method (Lactate Pro 2LT-1730; Arkray, Kyoto, Japan). The peak [La]b value was recorded as the highest value obtained after the end of the tests.
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The exercise protocol began between 10.30 a.m. and 11 a.m. to minimise the influence of circadian variation [29 (link)]. Heart rate at fixed blood lactate concentrations of 2 and 4 mmol·L−1 (FBLC2 and FBLC4, respectively) was assessed using an incremental exercise test on a bicycle suited to the height of the participant (Surprise, size large, Pinarello, Italy; Alpha 1.2, size 56 cm, Trek, Taiwan; Mira, size small, Litespeed, Ooltewah, TN, USA) mounted on a cycle ergometer (Computrainer Pro 3D, RacerMate, Seattle, WA, USA). Blood samples, taken from the ear lobe, were analysed for blood lactate concentration using the Lactate Pro 2 Meter (Lactate Pro2 LT-1730, Arkray, Kyoto, Japan), which has been previously reported to be a reliable (CV ≤ 1.0%) measure of blood lactate concentration [30 ]. The initial workload was set at 110 W increasing 30 W every 3 min. Samples were taken within the last 30 s of each workload until lactate concentration was ≥4 mmol·L−1. At this point the workload increased by 20 W every minute until volitional exhaustion or when cadence >60 rpm could not be maintained.
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Maximum oxygen uptake (Vo2 max) was measured by an incremental test with a treadmill using a respiratory instrument for measuring metabolism (AE-100i, Minato Medical Science Co Ltd., Tokyo, Japan). Measurements were initiated from 8 km/h at an inclination of 1% and then increased by 2 km/h every 3 min. After four stages, the gradient was increased by 1% every minute and this was continued until the subjects reached exhaustion.18 Heart rate was measured simultaneously during exercise, and the heart rate on going all out was designated the maximum heart rate. Blood lactate was analyzed using a blood lactate test meter (Lactate Pro2 LT-1730, Arkray, Kyoto, Japan), for which a trace amount of blood was collected from the fingertip directly, immediately after exercise.
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