AS05085
AS05085 is a laboratory equipment product. It is designed for specific laboratory applications.
Lab products found in correlation
11 protocols using AS05085
Glycine-SDS-PAGE Analysis of NPQ Proteins
Western Blot Analysis of Toxoplasma Proteins
For the blue native-PAGE, proteins were transferred onto a PVDF membrane (0.45 μm, Hybond) using wet transfer in Towbin buffer (0.025 M TRIS 0.192 M Glycine 10% Methanol) for 60 minutes at 100 V. After transfer, immunolabelling and visualisation was carried out as described above by chemiluminescence detection.
Quantitative Western Blot Analysis of Protein Expression
Protein Separation and Western Blot Analysis
Immunoblotting of Thylakoid Proteins
Immunoblot Analysis of Photosynthetic Proteins
Immunoblotting of Photosynthetic Proteins
Western Blot Analysis of Parasite Proteins
Protoplast Transformation and Chloroplast Protein Extraction
Chloroplast proteins were extracted from leaf tissues of 2-week-old WT, hsp82-1, and btf3-1 seedlings as described previously (Cho et al., 2006 (link)). Coomassie Blue staining was used as an internal loading control. The target protein, atpB, was detected by atpB antibody (AS05085, Agrisera). The nuclear protein histone and cytoplasmic protein GAPDH were detected by histone (D151717, Sangon Biotech) and GAPDH (D110016, Sangon Biotech) antibody, respectively.
Mitochondrial protein analysis by Western blotting
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