OP9 (RCB1124), TSt-4 (RCB2116), NIH 3T3 (RCB2767), NALM6 (RCB1933), and K562 (RCB0027) cells were purchased from RIKEN Bio Resource Center (Tsukuba, Ibaraki, Japan) within 10 years. OP9, TSt-4, and NIH 3T3 cells were cultured in minimum essential medium-alpha modification (αMEM; Thermo Fisher Scientific) supplemented with 20% FBS (Sigma-Aldrich) and 1% penicillin-streptomycin (Nacalai Tesque). NALM6 and K562 cells were cultured in RPMI1640 (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin–streptomycin as described previously (13 (link)). OP9-hDLL1 and K562-hCD19 cells have been described previously (11, 26 (link)). HEK293T and Expi293F cells were purchased from (ATCC) and Thermo Fisher Scientific within 10 years, respectively. These cells were cultured in DMEM (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin–streptomycin as described previously (1 (link)). All cell lines were regularly tested for Mycoplasma contamination using BioMycox Mycoplasma PCR Detection Kit (CellSafe) and were frozen down at early passages (<7) and used in the experiments within five passages after thawing. These cells were not further authenticated by our laboratory; however, routine confirmation of in vitro growth properties, morphology, and transfection efficiency (HEK293T and Expi293F) provided evidence of correct cell identity.
Penicillin streptomycin
Manufactured by Nacalai Tesque
Sourced in Japan, United States, France, Germany, United Kingdom
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of the antibiotics penicillin and streptomycin, which work to inhibit the growth of a wide range of bacteria.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (70 mentions)
Fetal bovine serum (fbs), by Merck Group (39 mentions)
L glutamine, by Nacalai Tesque (35 mentions)
Fetal bovine serum (fbs), by Biowest (28 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (27 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (23 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (18 mentions)
Dulbecco s modified eagle s medium, by Nacalai Tesque (17 mentions)
Rpmi 1640, by Nacalai Tesque (16 mentions)
Rpmi 1640 medium, by Nacalai Tesque (14 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (13 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (13 mentions)
B27 supplement, by Thermo Fisher Scientific (13 mentions)
Fetal bovine serum (fbs), by Nacalai Tesque (12 mentions)
Glutamax, by Thermo Fisher Scientific (10 mentions)
L glutamine, by Thermo Fisher Scientific (9 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (9 mentions)
Non essential amino acids (neaa), by Nacalai Tesque (8 mentions)
Non essential amino acid (neaa), by Thermo Fisher Scientific (8 mentions)
Dulbecco s modified eagle s medium dmem, by Nacalai Tesque (8 mentions)
330 protocols using penicillin streptomycin
1
Cell Line Culture and Maintenance Protocol
2
Cell Lines for Integrin Adhesion Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MLO-A5 cell line (mouse osteocytes) was obtained from Kerafast (Boston, MA, USA) and grown in MEM alpha (Thermo Fisher Scientific, Waltham, MA, USA) containing 5% fetal bovine serum (FBS; Equitech-Bio, Kerrville, TX, USA), 5% calf serum (Biowest, Riverside, MO, USA), and penicillin/streptomycin (Nacalai, Kyoto, Japan). A human monocytic cell line (THP-1) was purchased from ATCC (Manassas, VA, USA) and human lung epithelial cell lines (11-18 and QG-56) were kindly provided by Dr. Yoshihiro Miyahara (Mie University Medical School, Mie, Japan). The human breast cancer line MDA-MB-231 was from ATCC. β1-control (Scr.) and β1-KO clones of MDA-MB-231 generated in our laboratory [16 (link)] were used to validate the effect of β1 integrin on cell adhesion to S1 protein. Cells were grown in RPMI-1640 (Nacalai) containing 10% FBS (Equitech-Bio) and penicillin/streptomycin (Nacalai).
3
Cell Lines for SCLC Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human SCLC cell lines NCI-H69 (H69), NCI-H82 (H82) and NCI-H69AR (H69AR) were purchased from American Type Culture Collection. The H69AR cell line are cross-resistant to anthracycline analogs. The normal bronchial epithelial cell line, 16HBE14o- (16HBE) were kindly provided by Dr Gruenert (Head and Neck Stem Cell Laboratory, University of California, USA). The H69, H82 and H69AR cells were cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA), supplemented with 10% FBS (Sigma-Aldrich; Merck KGaA) and 1% penicillin-streptomycin (Nacalai Tesque, Inc.) while, the 16HBE cells were cultured in minimum essential medium (Sigma-Aldrich; Merck KGaA) supplemented with 10% FBS (Sigma-Aldrich; Merck KGaA) and 1% penicillin-streptomycin (Nacalai Tesque, Inc.). The cells were maintained at 37°C in a humidified atmosphere with 5% CO2. Stocks of these cells were prepared within five passages of receipt; cells used for experiments were passaged for <6 months.
4
Mouse T cell and Dendritic Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BW5147 cells [AKR/J mouse (H-2k)-derived T cell lymphoma cell line; purchased from Japanese Collection of Research Bioresources Cell Bank, Japan; JCRB9002], and DC2.4 dendritic cell line [29 (link)] were cultured in R-10 complete medium [RPMI 1640 medium (Nacalai Tesque) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Sigma-Aldrich) and 1% penicillin-streptomycin (Nacalai Tesque)]. The 293T cell line (human embryonic kidney-derived epithelial cells transformed with large T antigen, purchased from Riken, Japan; RCB2202) was cultured in D-10 complete medium [DMEM (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin-streptomycin]. Each cell was grown in 10 cm polystyrene tissue culture dishes at 37°C in a 5% CO2 incubator. All the T cells were prepared from the spleen of OT-I and C57BL/6 mice. Splenocyte suspensions were prepared by pushing the spleens through a cell strainer (Corning). After treatment with ACK lysis buffer for 5 min at room temperature (RT) to remove the red blood cells, the splenocytes were washed twice and re-suspended in R-10 complete medium.
5
Culturing Human Cervical Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human cervical cancer cell lines SKG-II, HeLa and HCS-2 were purchased from the JECB cell bank (National Institutes of Biomedical Innovation, Osaka, Japan). SKG-II cells were cultured in Ham′s F12 media supplemented with 10% fetal bovine serum (NICHIREI BIOSCIENCES INC. Tokyo, Japan) and 50 U/mL penicillin-streptomycin (Nacalai tesque, Kyoto, Japan). The HeLa and HCS-2 cells was cultured in Dulbecco's Modified Eagle Medium (Nacalai tesque) media supplemented with each 10% or 15% fetal bovine serum and 50 U/mL penicillin-streptomycin.
6
Culturing and Differentiating Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293T cells and 3T3-L1 mouse fibroblasts were purchased from ATCC and were maintained in DMEM (high glucose) (Nacalai Tesque) supplemented with 10% FBS (Thermo Fisher Scientific) and penicillin/streptomycin (Nacalai Tesque). The 3T3-L1 cells were differentiated into adipocytes by treatment with 2.5 μM dexamethasone (Sigma-Aldrich), 2 μM insulin (Sigma-Aldrich), 0.5 mM 3-isobutyl-1-methylxanthine (Nacalai Tesque), and 1 μM pioglitazone (Sigma-Aldrich) for 2 days. NCI-H295R cells (CRL-2128) were purchased from ATCC and maintained in DMEM/F12 (Thermo Fisher Scientific) supplemented with 1% ITS+ Premix (Corning), 2.5% Nu-Serum (Corning), and penicillin/streptomycin (Nacalai Tesque).
7
Cell Culture Protocols for Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa cells were purchased from the Human Science Research Resources Bank (Sennan‐shi, Japan) and maintained in DMEM (Wako) containing 10% FBS (Sigma‐Aldrich) and penicillin–streptomycin (Nacalai Tesque, Kyoto, Japan) at 37°C in a humidified atmosphere containing 5% CO2.
Lewis lung carcinoma (3LL) cells were maintained in RPMI‐1640 medium containing 10% FBS and penicillin–streptomycin at 37°C. 4T1 tumor cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI‐1640 medium containing 10% FBS and penicillin–streptomycin at 37°C.
Lewis lung carcinoma (3LL) cells were maintained in RPMI‐1640 medium containing 10% FBS and penicillin–streptomycin at 37°C. 4T1 tumor cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI‐1640 medium containing 10% FBS and penicillin–streptomycin at 37°C.
8
Cell Culture and Osteoclast Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell Line HEK293 cells (female) were cultured in Dulbecco's modified Eagles' medium (DMEM, GIBCO) supplemented with 10% (v/v) fetal bovine serum (FBS, Biosera), and penicillin-streptomycin mixed solution (Nacalai Tesque, 100 units/mL each). A549 cells (male) were cultured in RPMI 1640 medium (GIBCO) supplemented with 10% (v/v) fetal bovine serum (FBS, Biosera) and penicillin-streptomycin mixed solution (Nacalai Tesque, 100 units/mL each). All cells were maintained at 37 C under 5% CO 2 atmosphere. The bone marrow cells used for the osteoclast formation were derived from a five-week-old ddY male mice purchased from Sankyo Labo Service Co., Inc. (Tokyo, Japan). The bone marrow cells were cultured in alpha-mem medium (Sigma Aldrich) supplemented with 10% calf serum (Biosera), L-glutamine (0.292 g/L, Nacalai Tesque), M-CSF (50 ng/mL, R&D systems), and TGF-ß1 (1 ng/ mL, R&D systems) at 37 C under 5% CO 2 atmosphere.
9
Cell Culture Conditions of Common Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HuCCT1 and HEL cells were purchased from the Japanese Cancer Research Resources Bank (Japan). BM-hMSCs and hPBMCs were purchased from Lonza Group AG (Switzerland). HEK293 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) high-glucose GlutaMAX supplement (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin-streptomycin (Nacalai Tesque). HuCCT1 and HEL cells were maintained in RPMI 1640 medium (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin-streptomycin. BM-hMSCs were maintained in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and penicillin-streptomycin.
10
Culturing HeLa and A549 Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa (human cervical cancer) and A549
(human lung cancer) cell lines were purchased from the European Collection
of Authenticated Cell Cultures (ECACC, London, UK). HeLa cells were
cultured in Eagle’s minimum essential medium (Fujifilm Wako
Pure Chemical Corporation) containing 10% fetal bovine serum (Sigma-Aldrich,
St. Louis, MO, USA), 1% penicillin–streptomycin (Nacalai Tesque
Inc.), nonessential amino acids solution 1 mM (Nacalai Tesque Inc.),
and 1% sodium pyruvate solution (Nacalai Tesque Inc.) in a humidified
atmosphere containing 5% CO2 at 37 °C. A549 cells
were cultured in Ham’s F-12K medium (Fujifilm Wako Pure Chemical
Corporation) containing 10% fetal bovine serum (Sigma-Aldrich) and
1% penicillin–streptomycin (Nacalai Tesque Inc.) in a humidified
atmosphere containing 5% CO2 at 37 °C. The cells were
passaged twice a week until reaching 70–90% confluence.
(human lung cancer) cell lines were purchased from the European Collection
of Authenticated Cell Cultures (ECACC, London, UK). HeLa cells were
cultured in Eagle’s minimum essential medium (Fujifilm Wako
Pure Chemical Corporation) containing 10% fetal bovine serum (Sigma-Aldrich,
St. Louis, MO, USA), 1% penicillin–streptomycin (Nacalai Tesque
Inc.), nonessential amino acids solution 1 mM (Nacalai Tesque Inc.),
and 1% sodium pyruvate solution (Nacalai Tesque Inc.) in a humidified
atmosphere containing 5% CO2 at 37 °C. A549 cells
were cultured in Ham’s F-12K medium (Fujifilm Wako Pure Chemical
Corporation) containing 10% fetal bovine serum (Sigma-Aldrich) and
1% penicillin–streptomycin (Nacalai Tesque Inc.) in a humidified
atmosphere containing 5% CO2 at 37 °C. The cells were
passaged twice a week until reaching 70–90% confluence.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!