Blood samples were taken the morning of the laboratory session to determine baseline hormone levels for the laboratory session, including estradiol and progesterone. Five milliliters (mL) of blood were collected at the onset of the laboratory session to assess serum estradiol and progesterone. Estradiol and progesterone samples were analyzed using the ELISA kits (ALPCO; estradiol sensitivity 10 pg/mL; progesterone sensitivity 0.1 ng/mL). Cortisol samples were collected −30, −15, +5, +20, +40, and +60 min post-stress induction. Five mLs of blood were collected at each timepoint to assess plasma cortisol and collected in heparinized tubes for each assay. Each tube was placed on ice immediately following drawing and centrifuged. Cortisol was analyzed with the ELISA kit (ALPCO; sensitivity 0.4 μg/dl). Biochemical samples are centrifuged, aliquoted, and stored at −80C. All assays were conducted by the Core Laboratory of the Yale Center for Clinical Investigation.
Elisa kit
Manufactured by ALPCO
Sourced in United States, France
The ELISA kit is a laboratory assay used to detect and quantify specific proteins or other analytes in a sample. It utilizes antibody-antigen interactions and colorimetric or fluorometric detection methods to provide quantitative results.
Automatically generated - may contain errors
Lab products found in correlation
Elisa kit, by R&D Systems (11 mentions)
Elisa kit, by Thermo Fisher Scientific (4 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions)
Elisa kit, by Mercodia (3 mentions)
Alphatrak, by Abbott (3 mentions)
Elisa kit, by Cusabio (3 mentions)
Insulin, by ALPCO (3 mentions)
Bc 536, by Tanita (2 mentions)
Detection kits for total cholesterol, by Nanjing Jiancheng (2 mentions)
Advia centaur, by Siemens (2 mentions)
Immulite 2000, by Siemens (2 mentions)
Elisa kit, by Crystal Chem (2 mentions)
Collagenase 5, by Roche (2 mentions)
Sonic dismembrator, by Thermo Fisher Scientific (2 mentions)
Elisa, by Merck Group (2 mentions)
Infinite 200 pro, by Tecan (2 mentions)
Accu chek, by Roche (2 mentions)
Glucometer, by Roche (2 mentions)
Glucose, by Merck Group (2 mentions)
Adiponectin, by ALPCO (2 mentions)
254 protocols using elisa kit
1
Hormonal Stress Response Biomarkers
2
Hormonal Stress Response Biomarkers
3
Measurement of Serum Adipokines via ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Measurement of serum resistin was performed with an ELISA kit (R&D Systems, Inc., Minneapolis, USA) [18 (link)]. The intra-assay coefficient of variation (CV) was < 6% and the inter-assay CV was < 10%.
Serum RBP4 was analyzed using an ELISA kit (ALPCO Diagnostics, Salem, MA, USA) [19 (link), 20 (link)]. The intra-assay CV was 5% and the inter-assay CV was < 10%.
Serum adiponectin was determined using an ELISA kit (ALPCO DIAGNOSTICS, Salem, NH, USA) [21 (link)]. The intra-assay CV was 5% and the inter-assay CV was 5%.
Serum RBP4 was analyzed using an ELISA kit (ALPCO Diagnostics, Salem, MA, USA) [19 (link), 20 (link)]. The intra-assay CV was 5% and the inter-assay CV was < 10%.
Serum adiponectin was determined using an ELISA kit (ALPCO DIAGNOSTICS, Salem, NH, USA) [21 (link)]. The intra-assay CV was 5% and the inter-assay CV was 5%.
4
Plasma CORT and ACTH Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected in a tube with anticoagulant (sodium citrate: 0.5 ml of 3.8% solution per 4.5 ml of blood) from individuals in the experimental group, blood plasma was separated by centrifuging at 1,800 rpm for 10 min (Mohri and Rezapoor, 2009 (link)) and stored at −20°C for the estimation of hormones and neurotransmitters. The level of CORT and ACTH was estimated (ELISA kit, ALPCO Diagnostics, Salem, NH). The whole brain was dissected out, placed on ice, and then the prefrontal cortex (PFC) was removed (Heffner et al., 1980 (link)). The obtained PFC tissue (25 mg) was washed and then homogenized in the supplied buffer (50 μl), the supernatant was collected by centrifuging (10,000 rpm at 4°C) for 10 min and stored at −80°C. The level of PFC CORT was estimated using an ELISA kit (ALPCO Diagnostics, Salem, NH).
5
Glucose-Stimulated Insulin Secretion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure GSIS, we fasted isolated islets or dispersed islet cells for 20 min in KRB HEPES (KRBH) buffer containing 0.2% BSA and low glucose (2.8 mM for mouse islets; 1.67 mM for human islets) at 37°C. We then replaced the media with fresh low glucose KRBH, incubated the cultures for 40 min, collected the supernatants and replaced the media with high glucose (17.5 mM) KRBH. After 40 min of further incubation, we collected the supernatants and assayed the insulin using an ELISA kit (ALPCO).
To adjust secreted insulin levels with the intraislet insulin contents, we collected the cells after GSIS, dissolved them in 0.2 M HCl in 95% ethanol and then sonicated them. We then neutralized the islet lysates with 0.2 M NaOH, collected the supernatants and assayed the insulin using an ELISA kit (ALPCO). Secreted insulin amount (ng/10 islets/40 min) was divided by intra-islet insulin amount (ng/10 islets), and it was presented as % change of control.
To adjust secreted insulin levels with the intraislet insulin contents, we collected the cells after GSIS, dissolved them in 0.2 M HCl in 95% ethanol and then sonicated them. We then neutralized the islet lysates with 0.2 M NaOH, collected the supernatants and assayed the insulin using an ELISA kit (ALPCO). Secreted insulin amount (ng/10 islets/40 min) was divided by intra-islet insulin amount (ng/10 islets), and it was presented as % change of control.
6
Insulin Release Assay in Islets and INS-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assay insulin release in islets, groups of 10 islets in triplicate were preincubated and then stimulated with either 2.8 mM or 16.7 mM glucose in Krebs-Ringer buffer (135 mM NaCl, 3.6 mM KCl, 0.5 mM NaH2PO4, 0.5 mM MgSO4,1.5 mM CaCl2, 5 mM NaHCO3, 10 mM Hepes, and 0.1% BSA, pH 7.4). For insulin content measurement, islets were incubated overnight in acidic ethanol solution (75% ethanol and 25% CH3COOH) and then sonicated on ice (Sonic Dismembrator 500; Thermo Fisher Scientific). After centrifugation, supernatants were collected for measurement of insulin content using ELISA kit (ALPCO). To assay insulin secretion in INS-1 cells, 3 d after transfection, cells were preincubated for 1 h in Krebs-Ringer buffer containing 3 mM glucose at 37°C. Thereafter, cells were incubated with Krebs-Ringer buffer containing 3 mM or 25 mM glucose for another 1 h at 37°C. The supernatants were collected for measurement of insulin secretion using an ELISA kit (ALPCO).
7
Serum 25(OH)D Quantification Protocol
8
Prenatal-Postpartum Hormonal Analysis
9
Multiplex Assay of Gut Hormones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Insulin, leptin, GIP (Gastric inhibitory peptide), amylin, PYY (Peptide YY), and PP (Pancreatic polypeptide) were assayed using a human gut hormone multiplex kit according to the manufacturer's instructions (Millipore, Billerica, MA). Intra-assay variation was lower than 11%. Adiponectin levels were measured by radioimmunoassay (Millipore, Billerica, MA) and the intra-assay variation for this was from 6.90 to 9.25%. Total plasma ghrelin levels were measured using an ELISA assay kit from Millipore according to the manufacturer's instructions. Glucagon levels were assayed using a Millipore RIA kit. Brain-derived neurotrophic factor (BDNF), GH, Agouti-related protein (AgRP), and prolactin were measured with a Human Brain-Derived/Pituitary Protein Multiplex Panel assay kit according to the manufacturer's instructions (Millipore): intra-assay variation was lower than 10%. Cytokines were assayed using a human cytokine/chemokine assay kit according to the manufacturer's instructions (Millipore): intra-assay variation was lower than 10.5%. C-reactive protein (CRP) was measured using an ALPCO ELISA kit according to the manufacturer's instructions (ALPCO Diagnostics, Salem NH), and the intra-assay variation was 5.5–6.0%.
10
Metabolic Parameters Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 20
Samples from the ipGTT were assayed for insulin using an ELISA kit (Alpco Diagnostics, Salem, N.H.). GIP was assayed by Meso Scale Discovery human total GIP kit (validated for use with rat samples). Serum obtained at euthanasia was assayed for triglyceride (Serum Triglyceride Determination Kit, Sigma-Aldrich) and free fatty acids (Waco Diagnostics, Richmond, Va.) by colourimetric assays and active glucagon-like peptide-1 by ELISA (Millipore, Billerica, Mass.) according to manufacturers' instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!