Antibiotic antimycotic solution penicillin streptomycin amphoterichin b

Immortalized HAP 1 and MEF cell lines were used for this study.⁶ (link)^,8 (link) HAP 1 cells were grown Iscove’s Modified Dulbecco’s Medium (IMDM), supplemented with 10% Heat-Inactivated Fetal Bovine Serum (HI FBS; Life Technologies), 10 mL per L of Antibiotic Antimycotic Solution (Penicillin/Streptomycin/Amphoterichin B; Sigma Aldrich) and 2 mM L-Glutamine. MEF cells were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Cytiva) supplemented with 10% HI FBS, 10 mL per L of Antibiotic Antimycotic Solution, and 1X Non-Essential Amino acids (NEAA; Lonza). HAP 1 Δ (c+δ) cell line that lacks c and δ-subunits of ATP synthase was used for the study of the role of ATP synthase in high-conductance PTP. MEF ANT Triple KO cell line lacking 3 ANT genes was used to study the role of ANT in high conductance PTP. MEF ANT Triple KO cells were grown in the same media as MEF WT cells with the addition of 1mM Sodium Pyruvate (Gibco) and 25mg/500mL Uridine (Sigma Aldrich). Cells were maintained in a humidified cell incubator, at 37°C under a 5% CO2 atmosphere.