G418 sulfate
G418 sulfate is an antibiotic commonly used as a selection marker in cell culture experiments. It is effective against a wide range of eukaryotic cells, including mammalian, plant, and fungal cells. G418 sulfate functions by inhibiting protein synthesis, making it useful for selecting cells that have been successfully transfected or transduced with a resistance gene.
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7 protocols using G418 sulfate
Establishing Stable GFP-CD133 Cell Lines
Construction and Characterization of BDH1 Deletion Mutant
Yeast cells were cultured in YPD medium or in synthetic complete medium lacking histidine (SC-His) (20 or 50 g/L glucose, 6.7 g/L yeast nitrogen base without amino acids, and 1.92 g/L amino acids mixture lacking histidine).
Stable DUSP1 Knockdown in FK Replicon Cells
Antibiotics for Gene Selection
ANGPTL4 Overexpression Construct Generation
To produce mCherry expressing cells, melanoma cells were similarly transduced with a pQCXIN-mCherry plasmid and were selected using 800μg/ml G418 Sulfate (A.G. Scientific, Inc., San Diego, CA, USA).
OATP1B1 and Oatp1b2 Transporter Assay
Sorafenib and rifampin were obtained from Chemie Tek (Indianapolis, IN). General tritium‐labeled Sorafenib (specific activity, >1 Ci/mmol; radiochemical purity, >97.1%) was custom made by Moravek Biochemicals (Brea, CA), and [3H]estradiol‐17β‐D‐glucuronide (E2G; specific activity, 50.1 Ci/mmol; radiochemical purity, 99.0%), a positive control substrate for OATP1B1 and Oatp1b2, was obtained from American Radiolabeled Chemicals (St. Louis, MO).
RhoA Activation Assay and Apoptosis Analysis
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