Lhcb1
Lhcb1 is a protein subunit of the light-harvesting complex II (LHCII) in photosynthetic organisms. LHCII is the most abundant light-harvesting complex and plays a crucial role in capturing and transferring solar energy to the photosynthetic reaction centers.
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10 protocols using Lhcb1
Protein Analysis of Arabidopsis Leaves
Quantitative Protein Analysis by Western Blot
Protein Extraction and Detection in Barley Leaves
The proteins were separated by SDS-PAGE and transferred on to a nitrocellulose membrane. Proteins were stained with colloidal Coomassie G-250 (Dyballa and Metzger, 2009 ). For the immunological detection of proteins, polyclonal antibodies to HvPAP14 and HvPAP14i (BioGenes, Berlin, Germany), SAG12, RbcL, LHCB1, LHCB5, PSBO, and PsaA (all from Agrisera, Vännäs, Sweden) were used. Immunoreactive proteins were visualized with a peroxidase-coupled secondary antibody employing chemiluminescence (ECL Select Amersham, Pierce Therma Scientific Waltham, MA, USA; Ultra TMA-6 Lumigen, Southfield, MI, USA).
Arabidopsis Photosynthetic Protein Analysis
Thylakoid Protein Extraction and Analysis
Separation and Analysis of Thylakoid Protein Complexes
For immunoblotting, proteins were transferred on PVDF membrane (Millipore) and recognized by specific antibodies for LHCB1 (1:5000, Agrisera), LHCB2 (1:5000, Agrisera), PsaB (1:3000, Agrisera), STN7 (1:1000, Agrisera), and CP47 (1:3000, gift from Prof. Roberto Barbato). Phosphorylated threonine residues were recognized with p-Thr antibody (1:3000, New England Biolabs) and phosphorylated LHCB1 (p-LHCB1, 1:10000, Agrisera) and LHCB2 (p-LHCB2, 1:10000, Agrisera). For detection, horseradish peroxidase-linked secondary antibody (Agrisera) and Amersham ECL Western blotting detection reagents (GE Healthcare) were used.
Thylakoid Membrane Protein Isolation and Analysis
Thylakoid Membrane Protein Profiling
Immunoblotting Analysis of Photosynthetic Proteins
Immunoblotting Analysis of Photosynthetic Proteins
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