Additional data from peripheral blood samples used for TCR productive clonality comparisons were taken from the immuneACCESS open access database (Adaptive Biotechnologies). Henderson et al.42 (link) isolated, by FACS, CD4+CD25+CD127low Tregs from the peripheral blood (PB) of three young donors, whose ages ranged from 9.2–16.1 years. In the second study included, Emerson et al.61 (link) FACS-isolated CD4+CD45RA+CD62L+ (naive) and CD4+CD45RA−CD45RO+ (memory) T cells from 17 APB samples. Each study also used the immunoSEQ assay (Adaptive Biotechnologies) for TRB sequencing.
ImmunoSEQ Assay
The ImmunoSEQ Assay is a high-throughput DNA sequencing platform developed by Adaptive Biotechnologies. It is designed to analyze and quantify the adaptive immune repertoire, capturing the diversity of T-cell and B-cell receptors. The assay utilizes proprietary technology to amplify and sequence immune receptor DNA from biological samples, providing a comprehensive view of the adaptive immune system.
Lab products found in correlation
111 protocols using ImmunoSEQ Assay
Profiling T-cell Receptor Diversity
Additional data from peripheral blood samples used for TCR productive clonality comparisons were taken from the immuneACCESS open access database (Adaptive Biotechnologies). Henderson et al.42 (link) isolated, by FACS, CD4+CD25+CD127low Tregs from the peripheral blood (PB) of three young donors, whose ages ranged from 9.2–16.1 years. In the second study included, Emerson et al.61 (link) FACS-isolated CD4+CD45RA+CD62L+ (naive) and CD4+CD45RA−CD45RO+ (memory) T cells from 17 APB samples. Each study also used the immunoSEQ assay (Adaptive Biotechnologies) for TRB sequencing.
TCRB Deep Sequencing for Clonality Confirmation
Clonality was calculated according to the formula:
where pi is the proportional abundance of the rearrangement i, and N is the total number of rearrangements. The numerator of the equation is Shannon’s entropy. TCR repertoire overlap between two samples was calculated with the following formula:
in which ai is the template count of clone i in sample A, bi the template count of clone i in sample B, A the total number of templates in sample A, and B the total number of templates in sample B.
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