Mouse il 7 quantikine elisa kit

Male and female mice (dTG or Ctrl) were injected i.p. with 2 mg doxycycline (Dox, Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) and 0.5 mg Dexamethasone (Dex, Sigma-Aldrich Chemie GmbH) per 25 g body weight on five consecutive days. Serum IL-7 concentration was measured before and after Dex/Dox administration, using the Mouse IL-7 Quantikine ELISA Kit according to the manufacturer's protocol (R&D Systems, Minneapolis, MN, USA).
To transiently induce high levels of IL-7 in mice without an inducible IL-7 transgene, male and female C57BL/6.Foxp3^RFP/GFP mice and NOD.Rag1^−/− recipients were i.p. injected with IL-7/anti- IL-7 mAb immune complexes (IL-7/M25 mice). For this purpose, 15 μg recombinant human IL-7 (rhIL-7, carrier-free, R&D Systems) were incubated with 1.5 μg anti-IL-7 mAb (clone M25, BioXCell, West Lebanon, NH, USA). After 30 min at 37°C, complexes were diluted with PBS to obtain 200 μL of IL-7 complex solution per mouse.