The largest database of trusted experimental protocols

Rapamycin

Manufactured by Adooq
Sourced in United States

Rapamycin is a macrolide compound produced by the bacterium Streptomyces hygroscopicus. It functions as an immunosuppressant and has applications in research, particularly in the study of cell growth and proliferation. Rapamycin inhibits the mechanistic target of rapamycin (mTOR) signaling pathway, which plays a crucial role in regulating cellular processes such as metabolism, growth, and proliferation.

Automatically generated - may contain errors

5 protocols using rapamycin

1

Rapamycin Pretreatment for Heart Transplant

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rapamycin (A10782, AdooQ Bioscience, Irvine, CA USA) was dissolved according to the manufacturer’s instructions (2% DMSO, 30% PEG300, 5% Tween80, ddH2O. An amount of 8 mg/kg [18 (link)] Rapamycin, or the same volume vehicle, was administered via intraperitoneal injections at 12 h prior to graft procurement on the donor mice in Group I. Donor hearts were procured and stored in 4 °C HTK solution for 12 h before transplantation of the hearts to the recipients. The transplanted hearts were touched every day, and the survival curve for the donor hearts was recorded.
+ Open protocol
+ Expand
2

Chemical Treatments for Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Z-VAD-FMK, chloroquine (CQ), Rapamycin (Rapa), NU7441, Cyt387, A674563, KU60019, LY294002, PD0332991, AT7519, MK2206, CUDC907, LY2109761, GANT61, BIX 02189, Spautin-1, QNZ, LY317615, PD169316, GSK2606414, LYK974, TCK ERK 11e, SC79, MC1568, H89, ICG001, Perifosine, AEE788, ABT263, GDC-0941, FH535, PD0325025, NU7026, STF-083010, AEBSF HCl were purchased from Adooq. N-Acetyl-l-cysteine ethyl ester, DMSO, PEG300, and Tween-80 were purchased from MCE.
+ Open protocol
+ Expand
3

Statin-induced Cell Viability Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded into a 96-well plate (6 × 104/well) on F-12K medium and treated with statin (10–20 μM, Merck Millipore, Germany) for 48 h, beginning 24 h after seeding. WST-1 solution (10 μL, Roche, Germany) was added to each well and the cells were incubated for 1 h. The absorbance at 440 nm was measured using a microplate reader (FLUOstar Galaxy, Germany). In some experiments, the cells were pretreated with various inhibitors such as necrostatin-1 (Nec-1, an inhibitor of RIP1, 10 μM, Enzo Life Sciences, USA), necrosulfonamide (NSA, an inhibitor of MLKL, 1.0 μM, Millipore), bafilomycin A1 (Baf-A1, 2 nM, Sigma-Aldrich), 3,4-dihydro-5[4-(1-piperidinyl)butoxyl]-1(2H)-isoquinoline (DPQ, 60 μM, Calbiochem), Z-Val-Ala-Asp-fluoromethylketone (zVAD-fmk, 2 μM, BioVision, USA), and mTOR inhibitors (rapamycin, 20 nM, Adooq BioScience, USA) before statin treatment.
+ Open protocol
+ Expand
4

Pharmacological Interventions for Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Imatinib (Phoenix Pharmaceuticals, Belmont, CA, USA), 2‐deoxy‐d‐glucose (Wako, Osaka, Japan), CQ (Sigma, St Louis, MO, USA), metformin hydrochloride (LKT Laboratories, St Paul, MN, USA), rapamycin (Adooq Bioscience, Irvine, CA, USA), dasatinib (Cayman Chemical, Ann Arbor, MI, USA), bosutinib (KareBay Biochem, Ningbo, China), nilotinib (Cayman Chemical), ponatinib (Adooq Bioscience), methotrexate (Wako), cytarabine (LKT Laboratories), cisplatin (Sigma), vincristine sulfate (LKT Laboratories), STAT5 inhibitor (Calbiochem, San Diego, CA, USA), LY294002 (LC Laboratories, Woburn, MA, USA), and LY3900120 (Medchem Express, Princeton, NJ, USA).
+ Open protocol
+ Expand
5

Cell Culture Conditions for Osteosarcoma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines Saos-2 (HTB-85), SJSA-1 (CRL-2098), U-2 OS (HTB-96), MG-63 (CRL-1427), C3H/10T1/2 Clone 8 (CCL-226), and C2C12 (CRL-1772) were all purchased from the American Type Culture Collection and maintained in growth medium containing 10% FBS (Fisher Scientific, ES009B) and 1% Penicillin-streptomycin (Hyclone, SV30010) at 37°C under a humidified atmosphere containing 5% CO2. Unless stated otherwise, cells in 100-mm tissue culture dishes (Fisher Scientific, 430167) were treated with 1 ng/mL rapamycin (AdooQ Bioscience, A10782) for 24 h. The corresponding amount of dimethyl sulfoxide (DMSO) or ethanol was used as a vehicle control.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!