Total RNA was extracted from plant tissues and psyllids using RNAiso Plus (Takara, Japan). In a 20-μL volume, 1μg of total RNA was reverse transcribed with All-In-One 5X RT MasterMix (abm) following the manufacturer’s instructions. Quantitative RT-PCR (qRT-PCR) assays were conducted to analyze gene transcript level. Primer pairs of selected genes are listed in
All-In-One 5X RT MasterMix
The All-In-One 5X RT MasterMix is a ready-to-use solution for reverse transcription and subsequent PCR amplification. It contains all the necessary components for cDNA synthesis and PCR in a single tube, simplifying the workflow.
Lab products found in correlation
32 protocols using All-In-One 5X RT MasterMix
Quantitative Detection of 'Candidatus Liberibacter asiaticus'
Total RNA was extracted from plant tissues and psyllids using RNAiso Plus (Takara, Japan). In a 20-μL volume, 1μg of total RNA was reverse transcribed with All-In-One 5X RT MasterMix (abm) following the manufacturer’s instructions. Quantitative RT-PCR (qRT-PCR) assays were conducted to analyze gene transcript level. Primer pairs of selected genes are listed in
Quantitative RNA Analysis in HL-1 Cells
RNA Extraction and qPCR Analysis
RNA Isolation and cDNA Synthesis
Quantifying Gene Expression in Fungal Species
RNA Isolation and qRT-PCR Analysis
Quantitative RT-PCR protocol for gene expression
Gene Expression Analysis of Vertebrate Embryos
Total RNA Extraction from Uterus Caruncles
Expression Profiling of Sirtuins and FoxO3a
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