Microplate reader

Manufactured by Bio-Rad

Sourced in United States, China, Japan, Italy, Germany, United Kingdom, Switzerland, France, Canada, Netherlands, Australia, Belgium, India

The Microplate reader is a laboratory instrument used to measure the absorbance or fluorescence of samples in a microplate format. It can be used to conduct various assays, such as enzyme-linked immunosorbent assays (ELISA), cell-based assays, and other biochemical analyses. The core function of the Microplate reader is to precisely quantify the optical properties of the samples in a multi-well microplate.

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Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by Merck Group (286 mentions) Dimethyl sulfoxide (dmso), by Merck Group (194 mentions) Cck 8, by Dojindo Laboratories (183 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (176 mentions) Cck 8 solution, by Dojindo Laboratories (135 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (122 mentions) Mtt solution, by Merck Group (115 mentions) Cell counting kit 8 (cck8), by Beyotime (88 mentions) Cck 8, by Beyotime (86 mentions) Cck 8 reagent, by Dojindo Laboratories (82 mentions) Cck 8, by Bio-Rad (80 mentions) Cck 8 solution, by Beyotime (67 mentions) Cck 8 reagent, by Beyotime (65 mentions) 96 well plate, by Corning (64 mentions) Cell counting kit 8 cck 8, by Beyotime (61 mentions) Cck 8 kit, by Dojindo Laboratories (59 mentions) Cck 8 assay, by Dojindo Laboratories (53 mentions) Mtt assay, by Merck Group (36 mentions) Cck 8 assay, by Beyotime (34 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (34 mentions)

Spelling variants of this product

Model 680XR microplate reader (15 citations) 3550 Microplate Reader (13 citations) 450 microplate reader (6 citations) Benchtop microplate reader (4 citations) Vmax microplate reader (3 citations) Benchmark Plus™ Microplate Absorbance Reader (3 citations) Microplate ReaderBio-Rad microplate reader (Model 680; (3 citations) Synergy H1 microplate reader (2 citations) 400 microplate reader (2 citations) Model 6870 Microplate Reader (2 citations)

6 794 protocols using microplate reader

Quantifying Purine Metabolites and Energy Ratio

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Hypoxanthine is metabolized into xanthine, which is metabolized into uric acid by XOR. These intracellular metabolites were quantified using a xanthine/hypoxanthine assay kit (Abcam, Cambridge, UK). Following the protocols, a reaction mixture of samples and standards was incubated for 30 min, and we measured the OD at 570 nm using a microplate reader (Bio-Rad Laboratories).
An AMP colorimetric assay kit and ATP colorimetric assay (BioVision Inc., Milpitas, CA, USA) was used to calculate the ratio of intracellular AMP to ATP, a key regulator of AMPK phosphorylation and purine metabolism, according to the manufacturer’s protocols. The OD of samples for intracellular AMP and ATP levels were measured at 570 nm with a microplate reader (Bio-Rad Laboratories). The intracellular AMP/ATP ratio was normalized to that of LG as a control.

Yang K.J., Park H., Chang Y.K., Park C.W., Kim S.Y, & Hong Y.A. (2024). Xanthine oxidoreductase inhibition ameliorates high glucose-induced glomerular endothelial injury by activating AMPK through the purine salvage pathway. Scientific Reports, 14, 11167.

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Colistin Cytotoxicity Assessment in mRTECs

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Briefly, mRTECs were seeded at 1 × 10⁵ cells/well in 96-well plates and incubated for 12 h. After the cells were treated with a series dose of colistin for 2–48 h, the medium was discarded. 20 μL MTT solution (5 mg/mL, catalog number: M2128, Sigma-Aldrich) was added and the plates were incubated at 37°C for 4 h. Then, the medium was removed and 100 μL DMSO (catalog number: D2650, Sigma-Aldrich) was added to each well and incubated for 20 min at room temperature. Finally, the absorbance of each well was measured at 490 nm by a microplate reader (Bio-Rad, Hercules, CA, United States). LDH assay kit purchased from Nanjing Jiancheng Bio-Corporation (catalog number: A020-2-2, Jiangsu, China). LDH content was measured according to the manufacturer’s instructions. Briefly, after colistin treatment, the medium was collected and centrifuged at 3,500 rpm for 10 min at 4°C. The supernatant was collected and then the absorbance was measured with a microplate reader (Bio-Rad, Hercules, CA, United States) at 490 nm.

Wang J., Ishfaq M., Xu L., Xia C., Chen C, & Li J. (2019). METTL3/m6A/miRNA-873-5p Attenuated Oxidative Stress and Apoptosis in Colistin-Induced Kidney Injury by Modulating Keap1/Nrf2 Pathway. Frontiers in Pharmacology, 10, 517.

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Quantifying Cytokines and Nitric Oxide

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The levels of TNF-α, IL-1β and IL-6 in supernatants of claw dermal cells were detected using commercial ELISA kits, according to the manufacturer’s guidelines. The OD value at a wavelength of 450 nm was measured by a microplate reader (Bio-Rad, CA, USA). The NO concentration in dermal cell supernatant was measured using Griess colorimetric method, following the manufacturer’s protocol. Absorbance was measured at 550 nm by a microplate reader (Bio-Rad, CA, USA). Draw a standard curve with the standard solution concentrations as the horizontal axis and the measured OD value as the vertical axis, then calculate the concentrations of cytokines on the basis of standard curve. The intra- and inter- CV (coefficients of variations) were less than 15 %.

Tian M., Li K., Liu R., Du J., Zou D, & Ma Y. (2021). Angelica polysaccharide attenuates LPS-induced inflammation response of primary dairy cow claw dermal cells via NF-κB and MAPK signaling pathways. BMC Veterinary Research, 17, 248.

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Resveratrol Inhibits CAL-27 Cell Viability

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After CAL-27 cells had been treated with resveratrol (10, 20, or 40 μM) for 48 h, the culture medium was removed completely, and 100 μL of medium containing 10 μL of CCK-8 reagent was added to each well. Plates were incubated for 2 h, then optical density (OD) at 540 nm was measured using a microplate reader (Bio-Rad, Hercules, CA, USA). Relative cell viability (%) was calculated as OD_experiment/OD_control × 100%.
Treated cells were lysed in RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) and analyzed by BrdU ELISA based on the manufacturer’s instructions. OD at 507 nm was measured using a microplate reader (Bio-Rad).

Xiao Y., Duan Y., Wang Y, & Yin X. (2021). Resveratrol suppresses malignant progression of oral squamous cell carcinoma cells by inducing the ZNF750/RAC1 signaling pathway. Bioengineered, 12(1), 2863-2873.

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Quantification of Type I Collagen in Cell Supernatants

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HSC-T6 cells were seeded in six-well plates at a density of 5×10⁵/mL, and treated as described above in section “Treatments and groups.” The concentrations of collagen I in the supernatants were detected using ELISA kits according to the manufacturer’s guidance. Absorbance value was measured at 450 nm with the microplate reader (Bio-Rad Laboratories Inc., Hercules, CA, USA). The concentration of type I collagen was measured consistent with the corresponding standard curves. The OD value was measured at 450 nm on a microplate reader (Bio-Rad). The concentrations of collagen I were quantified according to the standard curves. The supernatant fluid was harvested and measured three times.

Ren H., Li Y., Chen Y, & Wang L. (2019). Endostatin attenuates PDGF-BB- or TGF-β1-induced HSCs activation via suppressing RhoA/ROCK1 signal pathways. Drug Design, Development and Therapy, 13, 285-290.

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Cell Proliferation Assays: CCK and BrdU

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For Cell Counting Kit (CCK) assay, a Cell Counting Kit reagent (Sigma-Aldrich, St. Louis, MO, USA) was added to the well and incubated for 2 h. Absorbance value was measured at 450 nm using a microplate reader (Bio-Rad Laboratories, Hercules, CA, USA). For BrdU incorporation, BrdU labeling reagent (Roche Diagnostics, Indianapolis, IN, USA) was added to each well and cells were reincubated for 24 h at 37°C. After removing CCM, cells were fixed and DNA denatured by adding 200 mL of FixDenat (Roche Diagnostics, Indianapolis, IN, USA) for 30 min at room temperature. After removing FixDenat, anti-BrdU-POD working solution was added to each well and left at room temperature on a shaker for 90 min. Each well's absorbance was measured at 370 nm with a microplate reader (Bio-Rad Laboratories, Hercules, CA, USA).

Wang J., Xiang B., Deng J., Freed D.H., Arora R.C, & Tian G. (2016). Inhibition of Viability, Proliferation, Cytokines Secretion, Surface Antigen Expression, and Adipogenic and Osteogenic Differentiation of Adipose-Derived Stem Cells by Seven-Day Exposure to 0.5 T Static Magnetic Fields. Stem Cells International, 2016, 7168175.

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Cell Proliferation and Invasion Assays

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Cell proliferation was evaluated using the MTT (Sigma, St Louis, MO) assay. Hep3B, MHCC97L and MHCC97H cells were seeded in 12-well culture plates at 0.5 × 10 5 /well, transfected with the indicated constructs or siRNA. The cells were incubated for 0, 24, 48, 72 and 96 h before adding the MTT reagent to each well at a final concentration of 0.5 mg/ml and incubated at 37 °C for 4 h. After medium removal, 500 μl of dimethyl sulfoxide was added to each well. Viable cells were measured by absorbance at a 550 nm wavelength using a microplate reader (BioRad, Hercules, CA).
Invasion were detected with a transwell migration and invasion assays. Cell invasion assay was performed with Matrigel (BD Biosciences, Sparks, MD) coated on the upper surface of the Transwell chamber (Corning, Lowell, MA). The cells that had migrated or invaded through the membrane were fixed with methanol and stained with crystal violet. Photographs of 3 randomly selected fields of the fixed cells were taken and cells were were measured by absorbance at a 550 nm wavelength using a microplate reader (BioRad, Hercules, CA).

Song L.J., Zhang W.J., Chang Z.W., Pan Y.F., Zong H., Fan Q.X, & Wang L.X. (2015). PU.1 Is Identified as a Novel Metastasis Suppressor in Hepatocellular Carcinoma Regulating the miR-615-5p/IGF2 Axis. Asian Pacific journal of cancer prevention : APJCP, 16(9).

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Biomarker measurement in blood samples

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Blood samples were centrifuged at 1000 g at 4 °C for 15 min, and the supernatants were collected and kept at −80 °C before biochemical analysis. Serum levels of CRP, ET and 6-K-PGF1α were determined by ELISA methods according to the instructions of commercial kits with a microplate reader (Bio-Rad, USA) under 450 nm. In addition, NO levels were determined by nitrate reduction met using the commercial detection kits according to the standard experimental protocols of the manufactures’ instructions with a microplate reader (Bio-Rad, USA) under 550 nm.

Zhang L., Yuan J.Q., Song F.C., Zhu M.D., Li Q., Liu S.H., Zhao K, & Zhao C. (2020). Ameliorative effects of the traditional Chinese medicine formula Qing-Mai-Yin on arteriosclerosis obliterans in a rabbit model. Pharmaceutical Biology, 58(1), 785-795.

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Cell Viability and Cytotoxicity Assay

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Cell viability and proliferation of each were measured by CCK8. Cells were rstly seeded into a 96-well plate, and after treatment, were treated with 10% CCK8 in 100 μL DMEM-High Glucose according to the manufacturer's protocols. Absorbance values were taken at 450 nm using a microplate reader (Bio-Rad, Hercules, CA, USA). The cytotoxicity of RA in each group was determined after 0, 7, and 14 days of cultivation. The supernatant of each was collected and detected using a LDH assay kit following the manufacturer's protocols. Absorbance values at 450 nm were detected using a microplate reader (Bio-Rad).

Li P.F., Xiong F., Xing H.Y., Hu S.J, & Zhang N. (2023). Retinoic acid inhibits the pyroptosis of degenerated nucleus pulposus cells by activating Sirt1-SOD2 signaling. Connective tissue research, 64(4).

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Cell Viability and Toxicity Assays

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Cell viability was evaluated by the CCK-8 Kit according to the manufacturer’s instructions (Beyotime, Cat# C0037). Briefly, SCs were aliquoted into 96-well plates at a density of 0.6 × 104 cells per well, and cultivated at 32 °C in a humidified atmosphere with 5% of CO2 until reaching approximately 70% confluence of cells. Treatment of cells with indicated treatment followed by addition of fresh culture media with 10 µL CCK-8 solution to each well, followed by incubating for 2 h. The absorbance was detected through the Microplate Reader (Bio-Rad, USA) at 450 nm to assess the viability of SCs. Toxicity of cells was evaluated through the content of lactate dehydrogenase (LDH) using LDH reagent kit (NJJCBIO, Cat# A020-2-2) following the instructions. The absorbance at 490 nm was measured using a Microplate Reader (Bio-Rad, USA).

Hu Y., Luo N.J., Gan L., Xue H.Y., Luo K.Y., Zhang J.J, & Wang X.Z. (2024). Heat stress upregulates arachidonic acid to trigger autophagy in sertoli cells via dysfunctional mitochondrial respiratory chain function. Journal of Translational Medicine, 22, 501.

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