Chirascan cd spectrometer
The Chirascan CD spectrometer is a laboratory instrument designed to measure the circular dichroism (CD) of samples. CD is a spectroscopic technique that provides information about the structural properties of chiral molecules, such as proteins and other biomolecules. The Chirascan CD spectrometer is capable of accurately measuring CD signals over a wide range of wavelengths, enabling researchers to study the conformation and folding of these molecules.
Lab products found in correlation
295 protocols using chirascan cd spectrometer
Amyloid Aggregation Kinetics Modulated by ATP
Structural Analysis of Rpgrip1l Domains
Quantifying Membrane Alignment via Linear Dichroism
Far UV-CD Analysis of Protein Structure
Spectroscopic Analysis of Natural Compounds
Spectroscopic Characterization of Compounds
CD Analysis of Peptide Release
Quantifying Fibrillar α-Synuclein using UV-CD
spectrometer was used to obtain UV-CD spectra of αS fibrils
prepared in PBS buffer at an effective protein concentration of 10
μM. This was measured as follows: after measurement of fibril
UV-CD spectra, 30% 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) was added
to the fibril suspension and shaken for 5 min. By measuring mean residue
ellipticity (MRE) values for known concentrations of monomeric αS
obtained after addition of 30% HFIP and shaking, a standard curve
of UV-CD signals versus monomer concentration was generated. From
this curve, unknown concentrations of 30% HFIP-solubilized fibrils
were estimated. Fibril samples were first purified using a 100 kDa
cutoff filter to remove monomeric protein. Spectra were recorded between
195 and 260 nm with a step size of 1 nm and a scanning speed of 10
nm/min using a 1 mm path length cuvette at room temperature.
Spectroscopic Characterization of Compounds
Spectroscopic Characterization of Compounds
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