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CNWBOND Carbon-GCB SPE Cartridge

Manufactured by ANPEL
Sourced in China

The CNWBOND Carbon-GCB SPE Cartridge is a solid-phase extraction (SPE) device designed for the purification and concentration of analytes. It features a sorbent bed composed of both carbon and graphitized carbon black (GCB) materials, which provide selective retention of target compounds. The core function of this product is to facilitate the extraction, cleanup, and pre-concentration of samples prior to instrumental analysis.

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82 protocols using CNWBOND Carbon-GCB SPE Cartridge

The freeze–dried stalks were crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz. 100mg powder was weighted and extracted overnight at 4°C with 0.6 ml 70% aqueous methanol. Following centrifugation at 10, 000 g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 ml; ANPEL, Shanghai, China, www.anpel.com.cn/cnw) and filtrated (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/) before UPLC-MS/MS analysis.
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The leaf and tuber samples of C. wenyujin were freeze-dried by a vacuum freeze-dryer (Scientz-100F) and then crushed by a mixer mill (MM 400; Retsch, Haan, Germany) with zirconia beads for 1.5 min at 30 Hz. Then, 100 mg of powder was extracted with 1.2 mL 70% methanol solution (v/v) at 4 °C overnight. After centrifugation to remove the undissolved residues, the extracts were absorbed by solid phase extraction with a CNWBOND Carbon-GCB SPE Cartridge (ANPEL, Shanghai, China), filtered using a SCAA-104 filter with a 0.22 μm pore size (ANPEL, Shanghai, China), and followed by LC–ESI−MS/MS analysis.
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The freeze-dried samples were crushed into powder with zirconia bead for 1.5 min at 30 Hz in a MM400-Retsch mixer mill. 100 mg powder was then extracted at 4 ℃ for 12 h in 70% MeOH (0.6 mL) followed by centrifugation for 10 min at 10,000 g. the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 ml; ANPEL, Shanghai, China) and filtered (0.22 µm) prior to UPLC-MS/MS analyses.
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Lyophilized fruit samples were pulverized using a mixer mill (MM 400, Retsch, Shanghai, China) with zirconia beads for 1.5 min at 30 Hz. The powdered samples (100 mg) powder was weighted and extracted with 1.0 mL 70% aqueous methanol. The sample was then stored at 4 °C overnight. During this time, the sample was vortexed six times to increase the extraction rate. Following centrifugation at 10,000× g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China) and filtrated (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China) before LC–MS analysis.
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The sample preparation, extract analysis, metabolite identification and quantification were performed at Wuhan MetWare Biotechnology Co., Ltd. (www.metware.cn) following their standard procedures and previously fully described by Yuan et al. (2018) and Wang et al. (2017) [31 , 32 (link)]. The freeze-dried sample was crushed using a mixer mill (MM 400, Retsch, Germany) with a zirconia bead for 1.5 min at 30 Hz. One hundred milligrams of powder was weighed and extracted overnight at 4°C with 1.0 mL 70% aqueous methanol. Following centrifugation at 10 000 × g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China, www.anpel.com.cn/cnw) and filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/) before LC-MS analysis.
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A freeze-dried sample (DF or FDF) was crushed using a mixer mill (MM400, Retsch) with a zirconia bead for 1.5 min at 30 Hz. One hundred milligrams of powder was weighed and extracted overnight at 4°C with 1.0 mL of 70% aqueous methanol. Following centrifugation at 10,000 g for 10 min, the extracts underwent cleanup and preconcentration (CNWBOND Carbon-GCB SPE cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China, www.anpel.com.cn/cnw) and filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/) before LC-MS analysis.
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Widely-targeted metabolomics was performed by Metware (Wuhan, China) as previously described6 (link). The freeze-dried plant samples were ground using a mixer mill (MM 400, Retsch) with zirconia beads at 30 Hz for 1.5 min. The powder was weighed (100 mg) and extracted with 1 mL 70% aqueous methanol overnight at 4 °C. After centrifugation at 10,000 × g for 10 min, the sample extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 ml; ANPEL, Shanghai, China) and filtered (SCAA-104, 0.22 μm pore size; ANPEL). Then the extracts were analyzed using an LC-ESI-MS/MS system.
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The leaf samples collected at 0, 1, 4, 8, 24, and 48 h were crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz. One hundred (100) mg powder was weighed and aliquots were extracted overnight at 4°C with 1 ml 70% aqueous methanol. Following centrifugation at 10,000 g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 ml; ANPEL, Shanghai, China, http://www.anpel.com.cn/Search.aspx?Types=6&Type=0&KeyWord=CnwBOND) and filtrated (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China) before the LC-MS analysis [22 (link)].
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The freeze-dried root was crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30Hz. One hundred milligrams of powder from each sample was extracted overnight with 1.0 ml 70% aqueous methanol at 4°C. Following centrifugation at 10,000 g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 ml; ANPEL, Shanghai, China, www.anpel.com.cn/cnw) and filtered with a nylon syringe filter (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/) before LC-MS analysis. Metabolite identification and quantification were performed at MetWare Biotechnology Co., Ltd. (Wuhan, China). Three biological replicates each were used for the metabolic studies of two radish varieties. The anthocyanin contents of the taproot flesh of the ZIXIN and BAIXIN plants were measured as previously described [25 (link)].
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Freeze-dried leaf samples were crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz. Afterward, 100 mg powder was extracted overnight at 4°C with 1.0 mL 70% aqueous methanol. Following centrifugation at 10000 x g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China) and filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China) before LC-MS analysis. The quantification of metabolites was performed following the multiple reaction monitoring (MRM) analysis procedures using a triple quadrupole-linear ion trap mass spectrometer (QTRAP), API 4500 Q TRAP LC/MS/MS System. Analyses were carried out as described previously [36 (link)], with each group having six biological replicates.
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