BSA
BSA is a laboratory product that serves as a standard protein solution. It is commonly used for various applications in biochemistry and molecular biology, such as protein quantification, enzyme activity assays, and Western blotting. The core function of BSA is to provide a reliable and consistent reference point for these types of experiments.
Lab products found in correlation
71 protocols using BSA
Western Blot Analysis of Apoptosis Markers
Immunofluorescence Labelling Protocol
Immunocytochemical Analysis of Chondrogenic Differentiation
Immunostaining of Virus-Infected Neurons
Immunofluorescence Staining of COX-2 in Cells
Leishmania-specific IgG Isotype Profiling
The
Leishmania-specific IgG isotype profile was determined with ELISA tests using SLA obtained from
L. majorpromastigotes. Three weeks after the termination of treatment,
plasma from all experimental mice was analyzed for the presence of IgG1- and IgG2a-specific antibodies. Plates (Sarstedt) were coated with 5 µg/mL of SLA diluted in carbonate buffer (15 mM
Na
2CO
3, 35 mM NaHCO
3), pH 9.6, and incubated overnight at 4 °C. Plates were blocked with 2% bovine serum albumin (AppliChem) and serial dilutions of plasma
samples (1/20 to 1/40 960) were added. Then, plates were incubated with biotinylated anti-mouse IgG1 (500 ng/mL) or IgG2a (250 ng/mL) (AbDSerotec), and HRP streptavidin solution (1/5000,
AbDSerotec) was added. Lastly, 3,3′,5,5′-tetramethylbenzidine substrate (ThermoFisher Scientific) was added, and absorbance was read at 450 nm. Results are expressed as antibody titer ± SD.
Antibody titer was determined as the maximum dilution in which the sampleʼs OD value was above the cutoff level (twice the mean value of blank wells).
Immunofluorescence Staining of Cells and Tissues
Cell Harvesting and Staining Protocol
chemostat culture, was harvested in mid-exponential phase (OD600 ≈ 0.5) and
centrifuged for 4 min at 4,000 g and 4°C. The supernatant was discarded, the
cells washed or stained, and inoculated (∼2 × 107 cells
ml−1) into M9 medium plus acetate, succinate, fumarate, or malate. For washing,
the cells were resuspended in 5 ml ice-cold M9 medium, centrifuged a second time, and re-suspended
in 1 ml of room temperature M9 medium. For staining, the cells were resuspended in 500 μl of
room temperature dilution buffer C (Sigma-Aldrich). A freshly prepared mixture of 10 μl PKH26
or PKH67 dye (Sigma-Aldrich) and 500 μl dilution of buffer C (both at room temperature) was
added. After 3 min at room temperature, 4 ml ice-cold filtered M9 medium containing 1% (w/v)
bovine serum albumin (AppliChem) was added. After centrifugation, the supernatant was discarded and
the cells washed twice.
Immunostaining of Mouse Embryonic Fibroblasts
Immunofluorescence Staining of Frozen Sections
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