Reversed phase analysis: Sample extracts were reconstituted in 100 µl of methanol and centrifuged for 4 min at 10,000 rpm and analyzed using an Agilent Technologies 1100 HPLC system (Agilent Technologies; Santa Clara, CA, USA) coupled to a Bruker Impact II TOF MS system (Bruker Corporation; Billerica, MA, USA) controlled by Bruker Hystar 3.2 Software. Eluent A was H2O with 0.1% formic acid and eluent B acetonitrile with 0.1% formic acid. The gradient was run with a flow rate of 500 µl*min-1 over a Phenomenex Kinetex C18 3 × 100 mm column, 2.6-µm particle size, 100 Å pore size (Phenomenex Inc., Torrance, CA, USA), and a Phenomenex Kinetex C18 guard column, both heated to 40°C in the LC oven. Samples were kept at 6°C in the sample rack until injection of 5 µl into the injection loop of the HPLC. Samples were eluted isocratically for 2 min with 5% of eluent B followed by a 25-min gradient to 95%, 95% for 5 min followed by 6-min equilibration at 5% of eluent B.
Impact 2 tof ms system
Manufactured by Bruker
Sourced in United States, Germany
The Bruker Impact II TOF MS system is a high-performance time-of-flight mass spectrometer designed for accurate and sensitive analysis of a wide range of samples. It provides reliable and reproducible results across various applications.
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Lab products found in correlation
2 protocols using impact 2 tof ms system
1
Reversed Phase HPLC-MS Analysis
2
LC-MS Analysis of Abi Metabolites
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LC separation was performed using a Nexera SFC/SFE-HPLC system (Shimadzu, Kyoto, Japan). A ZORBAX Eclipse Plus C18 column, Cosmosil 5C18-MR-II and Cosmosil 5C18-AR-II columns, whose temperatures were maintained at 40°C, and were used at a flow rate of 200 μL min -1 . The mobile phase consisted of (A) H2O and (B) 60 vol% methanol in acetonitrile. MS was performed using an impact II TOF/MS system (Bruker Daltonics, Bremen, Germany) equipped with an ESI source. MS was operated in the positive ion mode using the following operation parameters: source voltage, 500 V; capillary voltage, 4500 V; nebulizer pressure, 1.6 bar; drying gas, 8.0 L min -1 ; dry gas temperature, 200°C; collision induced dissociation energy, 0.0 eV; quadrupole ion energy, 5.0 eV; quadrupole low mass, 100 m/z; collision energy, 10.0 eV; transfer time, 30 -50 μs; pre pulse storage, 5 μs. The mass range was m/z 50 to 700. Nitrogen was used for the nebulizer, drying and collision gas. The concentrations of Abi and its metabolites were measured using the exact mass value of the protonated molecule ([M+H] + ) of each compound.
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