library preparation using the Chromium™ Single Cell Platform (10x
Genomics) as per the manufacturer’s protocol. Briefly, single cell
suspensions were partitioned into Gel Beads in Emulsion in the Chromium™
system (10x Genomics) at Yale Center for Genome Analysis, followed by cell lysis
and barcoded reverse transcription of RNA, cDNA amplification and shearing, and
5’ adaptor and sample index attachment. Final scRNA-seq libraries were
sequenced on an Illumina NextSeq 500 (WuXi AppTec, China).