HDPSCs

Manufactured by AllCells

Sourced in United States

HDPSCs are human-derived perivascular stem cells. They are multipotent stromal cells that can differentiate into various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin, Thermo Fisher Scientific (4 mentions) Streptomycin, Thermo Fisher Scientific (4 mentions) HEPES buffer solution, Thermo Fisher Scientific (4 mentions) GlutaMAX supplement, Thermo Fisher Scientific (4 mentions) Amphotericin B, Thermo Fisher Scientific (4 mentions) FBS, Thermo Fisher Scientific (1 mentions) DP0037F, Thermo Fisher Scientific (1 mentions) A3160802, Thermo Fisher Scientific (1 mentions)

6 protocols using HDPSCs

Culturing Human Dental Pulp Stem Cells

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Human dental pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC (Cat. DP0037F, Lot N° DPSC090411-01) were maintained in MEM α, GlutaMAX™ Supplement, no nucleosides (Gibco, 32561029), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, A3160802), 100 IU/mL penicillin, 0,1 mg/mL streptomycin (Gibco, 15140122), 2.05 µm/mL amphotericin B (Gibco, 15290026) and 10 mM HEPES buffer solution (Gibco, 15630122). All cells are maintained at 37 °C, 80% humidified atmosphere and 5% CO₂ environment. Campos et al. previously described the characterization of these cellular populations [34 (link)].

Guedes F., Branquinho M.V., Biscaia S., Alvites R.D., Sousa A.C., Lopes B., Sousa P., Rêma A., Amorim I., Faria F., Patrício T.M., Alves N., Bugalho A, & Maurício A.C. (2022). Gamma Irradiation Processing on 3D PCL Devices—A Preliminary Biocompatibility Assessment. International Journal of Molecular Sciences, 23(24), 15916.

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Human Dental Pulp Stem Cell Maintenance

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The human dental pulp stem/stromal cells (hDPSCs) used in this study were sourced from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). These were maintained in MEM α, GlutaMAX™ supplement, nucleoside-free (Gibco, 32561029). This medium was supplemented with 10% (v/v) foetal bovine serum (FBS) (Gibco, A3160802), 100 IU/mL penicillin, 0.1 mg/mL streptomycin (Gibco, 15140122), 2.05 µm/mL amphotericin B (Gibco, 15290026) and 10 mM HEPES buffer solution (Gibco, 15630122). DPSCs were maintained in standard conditions, namely at 37 °C in 80% humidified atmosphere and 5% CO₂.

Sousa A.C., Biscaia S., Alvites R., Branquinho M., Lopes B., Sousa P., Valente J., Franco M., Santos J.D., Mendonça C., Atayde L., Alves N, & Maurício A.C. (2022). Assessment of 3D-Printed Polycaprolactone, Hydroxyapatite Nanoparticles and Diacrylate Poly(ethylene glycol) Scaffolds for Bone Regeneration. Pharmaceutics, 14(12), 2643.

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Culturing Human Dental Pulp Stem Cells

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Human Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC, Alameda, CA, USA (Cat. DP0037F, Lot no. DPSC090411-01) were maintained in MEM α, GlutaMAX™ Supplement, no nucleosides (Gibco, 32561029), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, A3160802), 100 IU/mL penicillin, 0.1 mg/mL streptomycin (Gibco, 15140122), 2.05 µm/mL amphotericin B (Gibco, 15290026), and 10 mM HEPES buffer solution (Gibco, 15630122), Thermo Fisher Scientific, Waltham, MA USA. All the cells were maintained at 37 °C, 80% humidified atmosphere, and 5% CO₂ environment. A characterization study of these cells is described by Campos et al. [25 (link)].

Branquinho M.V., Ferreira S.O., Alvites R.D., Magueta A.F., Ivanov M., Sousa A.C., Amorim I., Faria F., Fernandes M.H., Vilarinho P.M, & Maurício A.C. (2021). In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study. International Journal of Molecular Sciences, 22(14), 7655.

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Culturing Human Dental Pulp Stem Cells

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hDPSCs were acquired from AllCells, LLC (Cat. DP0037F, Lot N° DPSC090411-01) and maintained in MEM α, GlutaMAX™ Supplement, no nucleosides (Gibco, 32561029), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, A3160802), 100 IU/mL penicillin, 0.1 mg/mL streptomycin (Gibco, 15140122), 2,05 µm/mL amphotericin B (Gibco, 15290026) and 10 mM HEPES buffer solution (Gibco, 15630122). Cells were maintained at 37 °C, 80% humidified atmosphere and 5% CO₂ environment. A previous work details the characterization of these cells [37 (link)].

Biscaia S., Branquinho M.V., Alvites R.D., Fonseca R., Sousa A.C., Pedrosa S.S., Caseiro A.R., Guedes F., Patrício T., Viana T., Mateus A., Maurício A.C, & Alves N. (2022). 3D Printed Poly( -caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on a Composite Preparation by Melt Blending or Solvent Casting Techniques and the Influence of Bioceramic Content on Scaffold Properties. International Journal of Molecular Sciences, 23(4), 2318.

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Expansion of Human Dental Pulp Stem Cells

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hDPSCs were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). Cells were thawed and expanded in vitro using standard protocols previously reported [17–29 (link)]. hPDSCs were maintained in αMEM, with GlutaMAX™, without nucleosides (32561029, Gibco^®) supplemented with 10% (v/v) fetal bovine serum (A31608-02, Gibco^®), 100 IU/ml penicillin, 0.1 mg/ml streptomycin (15140122, Gibco^®), 2.05 µg/ml amphotericin B (15290026, Gibco^®) and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid Buffer solution (15630122, Gibco^®), kept at 37°C and in a 95% humidified atmosphere with 5% CO₂.

Campos J.M., Sousa A.C., Caseiro A.R., Pedrosa S.S., Pinto P.O., Branquinho M.V., Amorim I., Santos J.D., Pereira T., Mendonça C.M., Afonso A., Atayde L.M, & Maurício A.C. (2018). Dental pulp stem cells and Bonelike® for bone regeneration in ovine model. Regenerative Biomaterials, 6(1), 49-59.

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Culturing Human Dental Pulp Stem Cells

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The hDPSCs (Allcells, Emeryville, CA, USA) were cultured in α-MEM containing 10% FBS and 1/100 volume of antibiotic liquid at 37°C and 5% CO 2. When the cells reached subconfluence, the cells were harvested using a 0.25% trypsin solution containing 0.02% EDTA and subcultured according to a conventional method. The hDPSCs subcultured 12-14 times were used in the experiment.

Ishigure H., Kawaki H., Shintani K., Ueno K., Mizuno-Kamiya M., Takayama E., Hotta M., Kondoh N, & Nikaido T. (2021). Effects of multi-components released from S-PRG filler on the activities of human dental pulp-derived stem cells. Dental materials journal, 40(6).

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