Ficoll-Hypaque
Ficoll-Hypaque is a density gradient medium used for the separation and isolation of cells, organelles, and other biological particles. It is a sterile, endotoxin-tested solution composed of Ficoll and sodium diatrizoate. The solution's specific gravity allows for the separation of different cell types based on their density.
Lab products found in correlation
23 protocols using Ficoll-Hypaque
Feline CD4+ and CD8+ T Cell Quantification
Isolation and Purification of MAIT Cells
Evaluating NK Cell Activity in Liver Diseases
PBMC Separation and Cell Isolation
Isolating Antigen-Specific CD4+ T Cells
Murine Myeloid-Derived Suppressor Cell Analysis
For the analysis of MDSCs, the appropriate number of cells was pre-incubated with optimal concentration surface marker antibodies (eBioscience), anti-mouse CD11b-APC, and anti-mouse Gr1-PE.
For the analysis of PMN-MDSCs and M-MDSCs, the appropriate number of cells was pre-incubated with optimal concentration surface marker antibodies (eBioscience), anti-mouse CD11b-APC, anti-mouse Ly6G-FITC, and anti-mouse Ly6C-PE.
For the analysis of immature or undifferentiated markers CD11c, F4/80, CD80, and MHCII on MDSCs, the appropriate number of cells was pre-incubated with optimal concentration surface marker antibodies (eBioscience), anti-mouse CD11b-APC, anti-mouse Gr1-PE or anti-mouse Gr1-FITC, and anti-mouse CD11c-FITC, anti-mouse F4/80-FITC, anti-mouse CD80-PE, or anti-mouse MHCII-PE.
Isolation of Peripheral Blood Mononuclear Cells
Isolation and Activation of PBMCs for EV Study
PBMCs in the majority of experiments were activated with 5 µg/mL phytohemoagglutinin (PHA) for 24 h prior to and during the phase of EV exposure. A volume corresponding to 8µg of proteins of EVs was added to the PBMC suspension (2 × 105 cells/200µL) for 4 days.
PBMCs were stained with the following kits: Muse™ Human CD8 T Cell Kit and Muse™ Human CD4 T Cell Kit (MIM100102 and MIM100101, Merck Millipore, Billerica, MA, USA). A minimum of 10,000 cells per sample was acquired and analyzed using Muse™ Cell Analyzer.
Isolation of CD34+ AML Progenitor Cells
Purification of Patient-Derived AML Cells
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