Ab187881

Manufactured by Abcam

Sourced in United States

Ab187881 is a recombinant monoclonal antibody that specifically binds to human AChE (Acetylcholinesterase). The antibody is produced in HEK293 cells.

Automatically generated - may contain errors

Lab products found in correlation

Alexa 488 or 546, by Thermo Fisher Scientific (2 mentions) Ab216875, by Abcam (2 mentions) Mab1435, by R&D Systems (2 mentions) Nanog, by ReproCELL (2 mentions) Oct4 c 10, by Santa Cruz Biotechnology (2 mentions) Glass bottom dish, by AGC Techno Glass (2 mentions) Triton x 100, by Merck Group (2 mentions) Tra 1 60, by Merck Group (2 mentions) Lsm 510 confocal laser scanning microscope, by Zeiss (2 mentions) Mab1924, by R&D Systems (2 mentions) Picro sirius red stain kit, by ScyTek Laboratories (1 mentions) Mab2018r 100, by R&D Systems (1 mentions) Nb100 2437, by Novus Biologicals (1 mentions) Af1121, by R&D Systems (1 mentions) Ab183685, by Abcam (1 mentions) Ab124824, by Abcam (1 mentions) Ab27591, by Abcam (1 mentions) Ab175388, by Abcam (1 mentions) Ab15285, by Abcam (1 mentions) Ab33875, by Abcam (1 mentions)

4 protocols using ab187881

Evaluating PRDM14 Expression in Pancreatic Lesions

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Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011). Mouse tissues were formalin‐fixed, paraffin‐embedded (FFPE), and sliced. IHC was performed as described previously 12. We used the following primary antibodies: human PRDM14 (ab187881) from Abcam Inc. (Cambridge, MA, USA) and mouse PRDM14 (#38965) from Signalway antibody (Pearland, TX, USA). The slides of mouse pancreatic tissues were also stained with hematoxylin and eosin (H&E) and Picro‐sirius red stain kit (ScyTek Laboratories, Inc., Logan, UT, USA) for collagen staining, according to manufacturer instructions.
By light microscopy, the tissue sections of TMA were scored semiquantitatively for PRDM14 staining, as described previously 12. Labeling scores were determined by multiplying the percentage of PRDM14‐positive cells per slide (0–100%) by the dominant staining intensity (0 = negative, 1 = trace, 2 = weak, 3 = intermediate, and 4 = strong). Resulting scores ranged from 0 to 400.
In this study, we used reported data on PRDM14 staining scores in normal pancreas, PDAC, and cancer adjacent tissues using TMA 12 to compare the expression levels with that in PanIN and chronic pancreatitis.

Moriya C., Imai K, & Taniguchi H. (2018). PRDM14 is overexpressed in chronic pancreatitis prior to pancreatic cancer. FEBS Open Bio, 8(10), 1733-1741.

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Comprehensive Stem Cell Marker Analysis

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The following primary antibodies were used in the study including anti-CXCR4 (ab124824, AbCam), anti-TRAIL (AF1121, R&D), anti-c-Kit (AF1356, Novus Biological), anti-CD47 (ab175388, Abcam), anti-Fasl (ab15285, Abcam), anti-PDL1 (NBP1-76769, Novus Biological), anti-CD4 (ab183685, Abcam), anti-SSEA1 (ab16285, Abcam), anti-Oct4 (ab184665, Abcam), anti-Sox2 (MAB2018R-100, R&D), anti-Nanos3 (ab70001, Abcam), anti-Ifitm3 (ab109429, Abcam), anti-Stellar (Invitrogen, PA5-34601), anti-PRDM14 (ab187881, Abcam), anti-Vasa (ab27591, Abcam), anti-DAZL (NB100-2437, Novus biologicals), anti-SMAD2 (ab33875, Abcam).

Liu C., Ma Z., Cai Z., Zhang F., Liu C., Chen T., Peng D., Xu X, & Lin H.K. (2020). Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models. Cell Discovery, 6, 15.

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Immunofluorescence Staining of Stem Cells

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Cells were cultured in a glass-bottom dish (AGC TECHNO GLASS, Shizuoka, Japan) and fixed with 4% paraformaldehyde for 10 min at 4 °C before being permeabilized with 0.1% Triton X-100 (Sigma) for 10 min at room temperature (RT). After blocking with 5% normal goat serum in Gibco™ Dulbecco's phosphate-buffered saline (DPBS; Thermo Fisher Scientific) for 30 min at RT, samples were incubated with primary antibodies at 4 °C overnight. After washing with DPBS, samples were incubated with secondary antibodies conjugated to Alexa 488 or 546 (Thermo Fisher Scientific) for 30 min at RT. After washing with DPBS, mounting medium with DAPI was used.
Primary antibodies specific for the following proteins were used in this study: OCT4 (C-10; Santa Cruz Biotechnology, Dallas, TX), NANOG (ReproCell), Tra 1-60 (MAB4360; Sigma–Aldrich), SSEA4 (MAB1435, R&D Systems), KLF4 (ab216875; Abcam), PRDM14 (ab187881; Abcam), anti-βIII Tubulin (TUJ1, Promega, Madison, Wisconsin), α-smooth muscle cell actin (α-SMA; A2547; Sigma), SOX17 (MAB1924; R&D Systems). Images were acquired using an LSM510 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany). All antibodies, except for the anti-TUJ1 antibody (1:300), were used at a 1:150 dilution in 5% normal goat serum.

Isono W., Kawasaki T., Ichida J.K., Ayabe T., Hiraike O., Umezawa A, & Akutsu H. (2020). The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human naïve-state pluripotency in non-hypoxic conditions. Regenerative Therapy, 15, 161-168.

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Immunofluorescence Staining of Stem Cells

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Cells were cultured in a glass-bottom dish (AGC Techno Glass, Shizuoka, Japan) and fixed with 4% paraformaldehyde for 10 min at 4 °C before being permeabilized with 0.1% Triton X-100 (Sigma-Aldrich) for 10 min at room temperature (RT). The samples were blocked with 5% normal goat serum in Dulbecco’s phosphate-buffered saline (DPBS; Thermo Fisher Scientific) for 30 min at RT. They were incubated with primary antibodies at 4 °C overnight. After washing with DPBS, the samples were incubated with secondary antibodies conjugated to Alexa 488 or 546 (Thermo Fisher Scientific) for 30 min at RT. After washing with DPBS, the samples were placed in mounting medium with 4',6-diamidino-2-phenylindole (DAPI). The following primary antibodies were used: OCT4 (C-10; Santa Cruz Biotechnology, Dallas, TX, USA), NANOG (ReproCell), Tra1-60 (MAB4360; Sigma-Aldrich), SSEA4 (MAB1435; R&D Systems), SSEA1 (MAB2155; R&D Systems), KLF4 (ab216875; Abcam), PRDM14 (ab187881; Abcam), anti-βIII Tubulin (TUJ1, Promega, Madison, Wisconsin, USA), α-smooth muscle actin (α-SMA; A2547; Sigma-Aldrich), and SOX17 (MAB1924; R&D Systems). Images were acquired using an LSM510 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany). All antibodies except for the anti-TUJ1 antibody (1:300) were used at a 1:150 dilution in 5% normal goat serum.

Isono W., Kawasaki T., Ichida J.K., Nagasaka K., Hiraike O., Umezawa A, & Akutsu H. (2023). Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells. Stem Cell Investigation, 10, 10.

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