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7 protocols using «13 c 12 bde 209»

1

Certified Standards for PBDE and HBCDD Analysis

2023
All solvents
used for sample preparation and gas and liquid chromatographic analysis
were of HPLC grade (Fisher Scientific, Loughborough, United Kingdom).
Individual standards of BDEs 28, 47, 99, 100, 153, 154, 183, 209,
77, 128, 13C12-BDE 100, and 13C12-BDE 209, as well as single standards of α-, β-,
and γ-HBCDD, d18-γ-HBCDD,
and 13C12-α-, β-, and γ-HBCDD
were purchased from Wellington Laboratories Inc., Ontario, Canada.
Two standard reference materials, European Reference Material for
polyethylene (ERM-EC-590) and polypropylene (ERM-EC-591) certified
for polybrominated diphenyl ether (PBDE) concentrations, were purchased
from the European Joint Research Centre Institute for Reference Materials
and Measurements (Brussels, Belgium). An in-house laboratory reference
material of extruded polystyrene with known concentrations of α-,
β-, and γ-hexabromocyclododecane (HBCDD) was obtained
from the National Institute for Environmental Studies (NIES, Tsukuba
City, Ibaraki, Japan). Cosmetics including sunscreen, antiperspirant,
moisturizing cream, and foundation were of popular commercial brands
purchased from a local UK supermarket.
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2

Comprehensive Analytical Methods for HBCD and BDE

2021
All solvents used for extraction and both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses were of HPLC grade (Fisher Scientific, Loughborough, UK). Concentrated sulfuric acid (99.7%) was purchased from Sigma-Aldrich (St. Louis, MA, USA).
Individual α-, β-and γ-HBCDD standards, 13 C12-α-, β-and γ-HBCDD, d18-γ-HBCDD, as well as individual standards of BDEs 28, 47, 77, 99, 100, 128, 153, 154, 183, 209, 13 C12-BDE 209, and 13 C12-TBBP-A were purchased from Wellington Laboratories (Guelph, ON, Canada). A full list of all target analytes are presented in the Table S1 of the supporting information.
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3

Quantitative Analysis of Halogenated Compounds

2020
HPLC-grade solvents were used for sample extraction and LC-MS/MS analysis (Fisher Scientific, Loughborough, UK). Concentrated sulphuric acid was purchased from Sigma-Aldrich (St. Louis, MA, USA). Individual α-, β-and γ-HBCDD standards, 13 C 12 α-, β-and γ-HBCDD, d 18 -γ-HBCDD, individual standards of PBDEs 17, 28, 47, 49, 77, 99, 100, 153, 154, 183,196, 197, 209 and 128, 13 C 12 -BDE-209,TBBP-A, 13 C 12-TBBP-A, PBBz, PBT, PBEB, HBB, and 13 C 6 -HBB and DBDPE were purchased from Wellington Laboratories (Guelph, ON, Canada).
Polychlorinated biphenyl (PCB)-129 was obtained from Qmx laboratories (Thaxted, UK). A certified reference material (CRM) for polypropylene (ERM-EC591), containing certified concentrations of PBDEs, was purchased from IRMM (Brussels, Belgium).
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4

Analytical Standards for Flame Retardants

2019
Standards of tributyl phosphate (TNBP), tris(2-ethylhexyl) phosphate (TEHP), triphenyl phosphate (TPHP), 2-ethylhexyl diphenyl phosphate (EHDPP), tris(2-isopropylphenyl) phosphate (TIPPP), tris(methylphenyl) phosphate (mixture of 3 isomers) (TMPP), TBOEP, TCEP, TCIPP, and TDCIPP were purchased from AccuStandard Inc., New Heaven, USA. The PBDE mixture (BDE-MXE), BEH-TEBP, EH-TBB, and the internal standards, 13 C 12 -BDE209, BDE58, TPHP-d15, TNBP-d27, TCEP-d12, TDCIPP-d15, were purchased from Wellington Laboratories Inc., Guelph, ON, Canada. The purity of analytical standards for OPFRs was >98%, except for TBOEP (>94%). Dust standard reference material (SRM 2585) was purchased from The National Institute of Standards and Technology (NIST) (Gaithersburg, MD, USA). The solvents and chemicals used were all analytical or HLPC grade, unless otherwise stated. Dichloromethane (DCM), methanol acetone, iso-octane, toluene, and n-hexane were purchased from J.T Baker, Deventer, The Netherlands. Whatman ® grade 541 filter paper, silica gel, and Florisil ® were purchased from Sigma-Aldrich (now Merck), Amsterdam, The Netherlands.
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5

Comprehensive Analytical Standards for Flame Retardants, Phosphates, and PAHs

2018
The BFR-PAR (Brominated Flame Retardant - native compounds stock solution) mixture [including BDE-7, 10, 15, 17, 28, 30, 47, 49, 66, 71, 77, 85, 99, 100, 119, 126, 138-140, 153, 154, 156, 169, 180, 183, 184, 191, 196, 197, 201, and 203-209, pentabromoethyl benzene (PBEB), hexabromobenzene (HBB), 1,2-bis(2,4,6-tribromophenoxy)ethane (TBE), and decabromodiphenyl ethane (DBDPE)], BDE-MXE (Brominated Diphenyl Ether – native solution mixture) solution mixture [including BDE-7, 15, 17, 28, 49, 47, 66, 71, 85, 99, 100, 119, 126, 138, 153, 154, 156, 169, 184, 183, 191, 196, 197, 206, 207, 209], and individual syn- and anti-DP, pentabromobenzene (PBBZ), tetrabromo-p-xylene (pTBX), 2-ethyl-hexyl tetrabromobenzoate (EHTBB), BDE-118, BDE-181, 13C12-BDE-209, di-(2-ethylhexyl)-tetrabromophthalate (BEHTBP) standards were purchased from Wellington Laboratories Inc. (Guelph, ON). Other individual compounds including BDE-77, -118, and -166 were purchased from AccuStandard, Inc. (New Haven, CT). The concentrations of PBDE/NFR calibration standard components ranged from 4 to 20 ng/mL.
Individual OPE standards [including tri-ethyl phosphate (TEP), tri-n-propyl phosphate (TPRP), tri-n-buytl phosphate (TNBP), tris(2-chloroethyl) phosphate (TCEP), tris[1-chloro-2-propyl] phosphate (TCIPP), tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), tris(2-butoxyethyl) phosphate (TBOEP), 2-ethylhexyl diphenyl phosphate (EHDPHP), tris(2-ethylhexyl) phosphate (TEHP), triphenyl phosphate (TPHP), tri-o-tolyl phosphate (TOTP), tri-m-tolyl phosphate (TMTP), tri-p-tolyl phosphate (TPTP), tris(2-isopropylphenyl) phosphate (TIPPP), tris(3,5-dimethylphenyl) phosphate (TDMPP), d12-tris(2-chlorethyl) phosphate (d12-TCEP), and 13C18-triphenyl phosphate (13C12-TPHP )] were also purchased from Wellington Laboratories, Inc. (Guelph, ON). Other individual OPE standards [including tris(2,3-dibromopropyl) phosphate (TDBPP), triisopropyl phosphate (TIPRP), and tripentyl phosphate (TPEP)] were purchased from AccuStandard, Inc. (New Haven, CT) and tris(4-tert-butylphenyl) phospate (TBPP) was purchased from Sigma Aldrich (St. Louis, MO).
The PAH solution mixture [including acenaphthene, acenaphthylene, anthracene, benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[ghi]perylene, benzo[a]pyrene, chrysene, dibenz[a,h]anthracene, fluoranthene, fluorene, indeno[1,2,3-cd]pyrene, phenanthrene, pyrene] and individual PAH standards [including d10-phenanthrene, d10-pyrene, d12-perylene, d12-benz[a]anthracene, and d12-anthracene] were purchased from Ultra Scientific (now Agilent Technologies, Santa Clara, CA) and individual PAH standards [including coronene, benzo[e]pyrene, and retene) were purchased from ChemService, Inc. (West Chester, PA). The concentration of each PAH calibration standard component was 20 ng/mL.
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