Corticosterone

Manufactured by Arbor Assays

Sourced in United States

Corticosterone is a laboratory reagent used for the quantitative determination of corticosterone levels in various biological samples, such as plasma, serum, or tissue extracts. It is a key component in assays designed to measure the concentration of this important glucocorticoid hormone.

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Lab products found in correlation

Testosterone, by Calbiotech (2 mentions) Leptin, by Crystal Chem (2 mentions) Insulin, by Crystal Chem (2 mentions) Insulin, by Merck Group (1 mentions) V plex proinflammatory panel 2 rat kit, by Mesoscale (1 mentions) Adiponectin, by Merck Group (1 mentions) Leptin, by Merck Group (1 mentions) β hydroxybutyrate, by Cayman Chemical (1 mentions) Corticosterone, by Thermo Fisher Scientific (1 mentions) Enzyme immunoassay kits, by ALPCO (1 mentions) Prolactin, by Merck Group (1 mentions) Igf 1, by R&D Systems (1 mentions) Estradiol, by Cayman Chemical (1 mentions) Testosterone, by Cayman Chemical (1 mentions) Progesterone, by Cayman Chemical (1 mentions) Phentolamine, by Merck Group (1 mentions) Pyruvate, by Merck Group (1 mentions) Propranolol, by Merck Group (1 mentions) Onetouch ultra, by Johnson & Johnson (1 mentions)

9 protocols using corticosterone

Multiplex Serum Biomarker Profiling in Rat Air Pollution Exposure

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Serum samples were measured for insulin (Millipore, Billerica, MA), alpha(1)-acid glycoprotein (AGP) and alpha-2-macroglobulin (A2M) (rat Acute Phase Protein Panel 1, Meso Scale Discovery, Rockville, MD). Alpha (1)-acid glycoprotein and alpha-2-macroglobulin are prominent acute phase proteins in rats (Cray et al. 2009 (link)). We also measured various cytokines in serum (i.e. TNF-alpha, IL-6, IL-1beta, IFN-gamma, IL-4, IL-5, IL-10, IL-13, and keratinocyte chemoattractant (KC)/growth-regulated oncogene (KC-GRO); V-PLEX Proinflammatory Panel 2 Rat kit, Meso Scale Discovery, Rockville, MD) according to the manufacturer’s protocol. We selected this panel of cytokines in part because we previously determined that exposure to air pollution modifies levels of IL-4 (Henriquez et al. 2017 (link)) and separately IL-6 and TNF-alpha (Henriquez et al. 2018 (link)). Plasma samples were used to measure adrenaline (Rocky Mountain Diagnostics, Colorado Springs, CO) and corticosterone (Arbor Assays, Ann Arbor, MI), according to the manufacturer’s protocol.

Martin B.L., Thompson L.C., Kim Y., Snow S.J., Schladweiler M.C., Phillips P., Harmon M., King C., Richards J., George I., Martin W.K., Haykal-Coates N., Gilmour M.I., Kodavanti U.P., Hazari M.S, & Farraj A.K. (2020). A Single Exposure to Eucalyptus Smoke Sensitizes Rats to the Postprandial Cardiovascular Effects of a High Carbohydrate Oral Load. Inhalation toxicology, 32(8), 342-353.

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Serum Biomarkers in Murine EAE

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In murine studies, serum samples were collected at baseline before
starting the diet (named T1), after four weeks on the diet but before
immunization (T2), and during clinical EAE (T3). Serum samples were
immediately frozen and aliquoted. The levels of corticosterone (Arbor
assays), adiponectin (EMD Millipore), leptin (EMD Millipore) and
β-Hydroxybutyrate (Cayman Chemical Company) were determined using
commercially available ELISAs. In the human study, commercially available
ELISA kits were used to test serum levels of leptin (EMD Millipore),
adiponectin (EMD Millipore), and β - Hydroxybutyrate (Cayman Chemical
Company).

Cignarella F., Cantoni C., Ghezzi L., Salter A., Dorsett Y., Chen L., Fontana L., Weinstock G.M., Cross A.H., Zhou Y, & Piccio L. (2018). Intermittent fasting confers protection in CNS autoimmunity by altering the gut microbiota. Cell metabolism, 27(6), 1222-1235.e6.

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Investigating Tumor Corticosterone Regulation

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MC38-Ova^dim was implanted in wild type C57BL/6 mice and either Metyrapone (50mg/kg; Fisher Scientific), Corticosterone (2.5mg/kg ) or vehicle control PBS (Gibco) was administered intra-tumorally on Day 5,6,7 and 9 post-tumor implantation. In some experiments, MC38-Ova^dim tumor explants or sorted lin⁻CD45⁺CD24⁻ cells were cultured in the presence or absence of Metyrapone (25 or 50 ng/ml) for 24hrs. Supernatants were harvested and Corticosterone measured by ELISA (Arbor Assays).

Acharya N., Madi A., Zhang H., Klapholz M., Escobar G., Dulberg S., Christian E., Ferreira M., Dixon K.O., Fell G., Tooley K., Mangani D., Xia J., Singer M., Bosenberg M., Neuberg D., Rozenblatt-Rosen O., Regev A., Kuchroo V.K, & Anderson A.C. (2020). Endogenous glucocorticoid signaling regulates effector differentiation and development of dysfunction in CD8+ T cells in the tumor microenvironment. Immunity, 53(3), 658-671.e6.

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Serum Hormone Profiling in Mice

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Blood was collected immediately after exposure on PND14 and also after the conclusion of social behavior testing (at approximately 12 months of age). Mice were weighed and then sacrificed by cervical dislocation without the use of sedatives and fresh brains removed and hemisected. Upon sacrifice, trunk blood was collected. Whole blood was collected into pre-chilled centrifuge tubes and centrifuged for 20 min at 3500 g for 20 min to obtain serum. Serum samples were stored at −20 °C until day of assay. Serum T and corticosterone were measured in duplicate using commercially available Enzyme immunoassay kits (testosterone: ALPCO, Salem, NH, USA; corticosterone: Arbor Assays, Ann Arbor, MI, USA), according to manufacturer’s specifications. Sample replicates with CV’s higher than 15% were excluded from analysis (two adult male samples were excluded).

Sobolewski M., Anderson T., Conrad K., Marvin E., Klocke C., Morris-Schaffer K., Allen J.L, & Cory-Slechta D.A. (2018). Developmental exposures to ultrafine particle air pollution reduces early testosterone levels and adult male social novelty preference: Risk for children’s sex-biased neurobehavioral disorders. Neurotoxicology, 68, 203-211.

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Serum Hormone Quantification by ELISA

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Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to determine the serum concentration of leptin (Crystal Chem), T4 (Calbiotech), testosterone (Calbiotech), corticosterone (Arbor Assays), GH (Millipore), IGF-1 (R&D Systems) and prolactin (Sigma).

Furigo I.C., Teixeira P.D., de Souza G.O., Couto G.C., Romero G.G., Perelló M., Frazão R., Elias L.L., Metzger M., List E.O., Kopchick J.J, & Donato J J.r. (2019). Growth hormone regulates neuroendocrine responses to weight loss via AgRP neurons. Nature Communications, 10, 662.

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Hormone Levels Quantification Protocol

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Commercially available ELISA kits were used to determine the serum concentration of leptin (Crystal Chem), T4 (Calbiotech), testosterone (Calbiotech), insulin (Crystal Chem), and corticosterone (Arbor Assays).

Ramos-Lobo A.M., Teixeira P.D., Furigo I.C., Melo H.M., de M Lyra e Silva N., De Felice F.G, & Donato J J.r. (2019). Long-term consequences of the absence of leptin signaling in early life. eLife, 8, e40970.

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Steroid Extraction from Tissues

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Organic phase extraction using acetonitrile and hexane (1:2) was employed to extract steroids from tissues followed by examination of corticosterone (Arbor Assays) and/or pregnenolone (Abnova) by ELISA.

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Thymus Steroid Secretion Assay

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Thymi were dissected into ice-cold PBS, and adipose and connective tissue were removed, transferred into about 5 ml of steroid-free culture medium [RPMI 1640 supplemented with 10% charcoalstripped heat-inactivated FBS, 2 mM l-glutamine, 1 mM sodium pyruvate, 25 mM Hepes, 0.1 mM nonessential amino acids, and gentamicin (50 mg/ml)], and minced into 1-mm³ pieces using forceps and scalpel. Fragments were left for at least 10 min to deplete endogenous steroid substrates and then transferred with forceps to 24-well plates containing 400 ml of steroid-free culture medium. Samples were cultured for 72 hours at 37°C in 5% CO₂, and then supernatants were collected and immediately assayed or stored at −20°C until assayed for steroids. Steroids were quantified using commercial enzyme-linked immunosorbent assay (ELISA) kits (corticosterone, Arbor Assays, K014; progesterone, Cayman Chemical, 582601; testosterone, Cayman Chemical, 582701; estradiol, Cayman Chemical, 501890) according to the manufacturer’s protocol, with standards diluted in steroid-free culture medium. One outlier subject, with all steroid measurements greater than threefold higher than all other subjects, was omitted from these analyses. Inclusion of this subject did not alter the results of these experiments.

Taves M.D., Donahue K.M., Bian J., Cam M.C, & Ashwell J.D. (2023). Aire drives steroid hormone biosynthesis by medullary thymic epithelial cells. Science immunology, 8(86), eabo7975.

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Fasting Impacts on Glucose Homeostasis

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Blood glucose levels were determined after 24 h and 48 h of fasting using a glucometer (One Touch Ultra; Johnson & Johnson, New Brunswick, NJ, USA). Glycemia at the fed state was also determined in mice without access to food for 4 h to standardize the postprandial state. Enzyme-linked immunosorbent assays were used to determine the serum concentrations of corticosterone (Arbor Assays, Ann Arbor, MI, USA), GH (Millipore, Billerica, MA, USA) and insulin (Crystal Chem, Downers Grove, IL, USA). These assays have a limit of detection determined as 20.9 pg/mL, 0.07 ng/mL and 0.1 ng/mL, respectively, and intra- and inter-assay coefficients of variability ≤ 10%. pyruvate tolerance tests (0.5 g pyruvate/kg; Sigma-Aldrich, St. Louis, MO, USA) were performed in mice at the fed state (4 h of food removal) or after 24 h and 48 h of fasting. The changes in blood glucose levels induced by an i.p. injection of 2DG (0.5 g/kg; Sigma-Aldrich) were also determined. The participation of the autonomic nervous system in the responses to these tests was determined by the co-infused of pyruvate or 2DG with either a muscarinic receptor blocker (3 mg/kg atropine; Sigma-Aldrich) or a combination of α and β receptor blockers (3 mg/kg phentolamine and 0.5 mg/kg propranolol, respectively; Sigma-Aldrich).

Pedroso J.A., da Silva I.B., Zampieri T.T., Totola L.T., Moreira T.S., Taniguti A.P., Diniz G.P., Barreto-Chaves M.L, & Donato J J.r. (2022). SOCS3 Ablation in Leptin Receptor-Expressing Cells Causes Autonomic and Cardiac Dysfunctions in Middle-Aged Mice despite Improving Energy and Glucose Metabolism. International Journal of Molecular Sciences, 23(12), 6484.

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