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K2 edta

Manufactured by Wiener Lab
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About the product

K2-EDTA is a laboratory reagent used for the collection and stabilization of blood samples. It acts as an anticoagulant, preventing the blood from clotting during sample processing and analysis. K2-EDTA is a common component in vacutainer tubes and other blood collection devices.

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Trizol, by Thermo Fisher Scientific (2 mentions)
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Similar products (other manufacturers)

K2 EDTA (by Innovative Research) - 11 citations K2 EDTA (by BectonDickinson Vacutainer Systems) - 9 citations K2-EDTA (by Medlab) - 4 citations K2 EDTA (by Teklab) - 3 citations K2 EDTA (by Deltalab) - 3 citations K2-EDTA (by SeraLabs) - 2 citations K2 EDTA (by SAI Infusion Technologies) - 2 citations

The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
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4 protocols using «k2 edta»

1

Plasma Biomarkers in Suckling Mice

Cited 1 time 2020
Suckling mice were euthanized at 8 days PI and blood samples were collected in tubes containing K2‐EDTA (Wiener lab). After centrifugation at 2000× g for 10 minutes at room temperature, plasma samples were separated. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), and lactate dehydrogenase (LDH) were measured by UV kinetics‐, kinetics‐ or enzymatic‐ method at 8 days PI using an Architect 8100 chemistry analyzer (Abbott Laboratories). Platelet counts were measured by microscopy on a CELLDYN 3700 (Abbott Laboratories) according to the manufacturer´s instructions.
Transaminitis was defined as ALT and/or AST plasma values significantly above the ones obtained for control mice. Plasma values of LDH and CK significantly higher than the values obtained for control mice were considered outcomes of disease. Platelet count significantly lower than the value obtained for control mice was also considered an outcome of disease.
Byrne A.B., García A.G., Brahamian J.M., Mauri A., Ferretti A., Polack F.P, & Talarico L.B. (2020). A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice. Animal Models and Experimental Medicine, 4(1), 16-26.
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2

Quantifying Viral RNA in Blood and Tissues of DENV-2 Infected Mice

2020
Blood was extracted from suckling mice at different times (0, 17, 24, 48, 72, 96 and 168 hours) after DENV‐2 infection and collected in tubes containing K2‐EDTA (Wiener lab.). RNA was extracted from plasma samples using QIAamp Viral RNA Minikit (QIAGEN) according to the manufacturer´s instructions.
After blood extraction at the corresponding post‐infection times, suckling mice were euthanized and the mesenteric lymph nodes as well as a portion of the tissues (liver, kidney, spleen, small intestine, and brain) were immediately snap‐frozen in tubes containing a mixture of dry ice/ethanol and later stored at −80°C. Total RNA was extracted from tissues using TRIzol (Invitrogen) according to the manufacturer´s instructions.
The amount of viral RNA in blood and tissues was quantified by using StepOnePlus Real‐Time PCR System (Applied Biosystems) employing TaqMan technology, as described previously.40 Beta‐actin was used as endogenous control. Standard curves were generated using 10‐fold serial dilutions of viral RNA obtained from purified DENV‐2 suspensions.
Byrne A.B., García A.G., Brahamian J.M., Mauri A., Ferretti A., Polack F.P, & Talarico L.B. (2020). A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice. Animal Models and Experimental Medicine, 4(1), 16-26.
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3

Quantifying DENV RNA in Blood and Tissues

2017
Blood was extracted at different times (0, 6, 18, 24, 48, 72, 96 and 168 h) after DENV infection and collected in tubes containing K2-EDTA (Wiener lab.). RNA was extracted from plasma samples using QIAamp Viral RNA Minikit (QIAGEN) according to the manufacturer's instructions.
After blood extraction at the corresponding post-infection times, mice were euthanized by cervical dislocation. The lymph nodes (mesenteric, periportal and celiac) as well as a portion of the tissues (spleen, liver, kidney) were immediately snap-frozen in tubes containing a mixture of dry ice/ethanol and later stored at − 80 °C. Total RNA was extracted from tissues using TRIzol (Invitrogen) according to the manufacturer's instructions.
The amount of viral RNA in blood and tissues was quantified by using StepOnePlus Real-Time PCR System (Applied Biosystems) employing TaqMan technology. The primers and probe used are targeted to amplify nucleotides 10,589 to 10,699 within the viral 3′UTR (Callahan et al., 2001 (link)). Beta-actin was used as endogenous control. Standard curves were generated using 10-fold serial dilutions of viral RNA obtained from purified DENV-1 or DENV-2 suspensions.
Talarico L.B., Batalle J.P., Byrne A.B., Brahamian J.M., Ferretti A., García A.G., Mauri A., Simonetto C., Hijano D.R., Lawrence A., Acosta P.L., Caballero M.T., Paredes Rojas Y., Ibañez L.I., Melendi G.A., Rey F.A., Damonte E.B., Harris E, & Polack F.P. (2017). The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection. EBioMedicine, 20, 202-216.
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4

Measuring Liver Enzymes and Platelet Counts in DENV-2 Infected Mice

2017
Blood samples were obtained from the facial vein of mice, collected in tubes containing K2-EDTA (Wiener lab), and after centrifugation at 2000 × g for 10 min at room temperature, plasma samples were separated. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), and lactate dehydrogenase (LDH) were measured by UV- or -kinetics method at different days post-secondary infection with DENV-2 (0, 4, and 7 days) using an Architect 8100 chemistry analyzer (Abbott Laboratories). Platelet counts were measured by microscopy on a CELLDYN 3700 (Abbott Laboratories) according to the manufacturer's instructions.
Increase of liver enzymes was defined as ALT and/or AST plasma values significantly above the ones obtained for control mice. Platelet counts significantly lower than the value obtained for control mice were also considered a primary outcome of disease. Plasma values of CK, LDH and ALP significantly higher than the ones obtained for control mice were considered secondary outcomes of disease. All normal laboratory values for C57BL/6 mice were obtained from the Comparative Pathology Laboratory, Vanderbilt University Medical Center, and the Mouse Phenome Database at The Jackson Laboratory.
Talarico L.B., Batalle J.P., Byrne A.B., Brahamian J.M., Ferretti A., García A.G., Mauri A., Simonetto C., Hijano D.R., Lawrence A., Acosta P.L., Caballero M.T., Paredes Rojas Y., Ibañez L.I., Melendi G.A., Rey F.A., Damonte E.B., Harris E, & Polack F.P. (2017). The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection. EBioMedicine, 20, 202-216.
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