TranScriba Kit
The TranScriba Kit is a laboratory equipment product designed for RNA extraction and purification. It provides a standardized protocol and necessary reagents to isolate high-quality RNA from various biological samples.
Lab products found in correlation
35 protocols using TranScriba Kit
Cyanobacterial 16S rRNA Sequencing Protocol
Quantifying ABCB1 mRNA Expression
RT-qPCR Analysis of 3T3-L1 Adipogenesis
PPARγ:
(+)5′-GCCCTTTGGTGACTTTATGG-3′, (−) 5′-CAGCAGGTTGTCTTGGATGT-3′;
C/EBP:
(+)5′-TTGCACCTCCACCTACATCC-3′, (−) 5′-CCACAAAGCCCAGAAACCTA-3′. The relative amount of each gene was calculated using the 2−ΔΔCt method.
Transcriptomic Analysis of P. aeruginosa
Expression changes for selected genes were confirmed by RT-qPCR using RNA isolated from independent cultures. In total, 1 µg of each RNA sample was used for reverse transcription (TranScriba Kit, A&A Biotechnology, Gdańsk, Poland) with the use of random hexamers. Three technical replicates of PA2577 overproducer and EV control were used. The nadB was used as the reference. RT-qPCR was also used for examination of the expression of PA2577 and PA2576 in the early (OD600~0.5) and late exponential (OD600~1.5) phases of growth of P. aeruginosa WT cultures and for PA2576 also in P. aeruginosa ΔPA2577 strain. The rpsL gene was used as the reference. Relative gene expression was calculated using the Pfaffl method [80 (link)]. All oligonucleotides used in RT-qPCR are listed in
Apoptosis Pathway Gene Expression
Staphylococcus aureus Gene Expression
and purification, reverse transcription, and qPCR were
performed according to previously published data.27 (link) Briefly, RNA was isolated and purified from the S. aureus 5 N isolate (OD600 = 0.5) after
samples were treated with sublethal doses of aPDI (reduction in bacterial
cell count ∼0.5 log10 units) using a Syngen Blood/Cell
RNA Mini Kit (Syngen, Poland). The TranScriba kit (A&A Biotechnology,
Poland) was used to transcribe the RNA to complementary DNA (cDNA).
qPCR assays were performed using a LightCycler 480 II (Roche Life
Science, Germany). The reaction mixture (10 μL total) consisted
of 5 μL of Fast SG qPCR Master Mix (EURx, Poland), 200–400
nM of each primer (TIB MOLBIOL, Germany) (
of cDNA. The following steps were implemented (
primer-dimer formation or nonspecific amplification. Relative changes
in the expression of the sec, tst, srrA, and srrB genes were normalized
to the gmk reference gene.
RNA Extraction and Reverse Transcription
Biochemical Assay Reagents and Materials
The water used for the analysis was deionized with the use of a Direct-Q Ultrapure UV3 Water System (EMD Millipore, USA). The water, media, sodium saline, and surgical instruments were sterilized using a Classic Standard Prestige Medical autoclave (Ma-Je-R, Poland).
RNA Extraction and RT-PCR Analysis of Chondrogenic Markers
Reverse Transcription of RNA to cDNA
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