Adult rats were randomly divided into three groups: control group (n = 21), BoNT/A group (n = 21), and agrin-Ab groups. BoNT/A (BOTOX®, Allergan, Co. Mayo, Ireland) was reconstituted in saline (NS) to a final concentration of 2 U/100 μL. Animals of the BoNT/A and agrin-Ab groups were injected unilaterally with 100 μL BoNT/A in the right gastrocnemius muscle under anesthesia with an intraperitoneal injection of pentobarbital (30 mg/kg). On the 3rd day after BoNT/A injection, the each subgroups of agrin-Ab were injected with 100 μL agrin-Ab (R&D Systems, Minnesota, CA, United States) at a dosage of 0.6, 2, 6, 20, or 60 μg respectively at once. Controls received an equivalent volume of NS injections in the right gastrocnemius muscle.
BoNT-A
BoNT-A is a highly purified form of the Botulinum Neurotoxin Type A. It is a biological product used in research applications. BoNT-A functions by inhibiting neurotransmitter release at the neuromuscular junction.
Lab products found in correlation
18 protocols using BoNT-A
BoNT/A and Agrin-Ab Effects on Rat Muscle
Adult rats were randomly divided into three groups: control group (n = 21), BoNT/A group (n = 21), and agrin-Ab groups. BoNT/A (BOTOX®, Allergan, Co. Mayo, Ireland) was reconstituted in saline (NS) to a final concentration of 2 U/100 μL. Animals of the BoNT/A and agrin-Ab groups were injected unilaterally with 100 μL BoNT/A in the right gastrocnemius muscle under anesthesia with an intraperitoneal injection of pentobarbital (30 mg/kg). On the 3rd day after BoNT/A injection, the each subgroups of agrin-Ab were injected with 100 μL agrin-Ab (R&D Systems, Minnesota, CA, United States) at a dosage of 0.6, 2, 6, 20, or 60 μg respectively at once. Controls received an equivalent volume of NS injections in the right gastrocnemius muscle.
BoNT-A Injection for Masseteric Hypertrophy
Standardized Botulinum Toxin Injections for Facial Aesthetics
Botulinum Toxin Injection in Spastic Gait
Submandibular Gland Botulinum Toxin Injection and Duct Ligation
Bilateral submandibular duct ligation was also performed under general anaesthesia. The submandibular ducts were traced, dissected, and ligated, applying two metal vascular clips to each duct. Intraoral absorbable sutures closed the incision.
Submandibular Gland Botox Injection
BoNT-A Administration for Induced OAB
BoNT-A Injection Technique for Masticatory Muscles
Botox Injection Protocol for Masticatory Muscles
Intravesical BoNT/A Injection in Rats
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