Fasudil
Fasudil is a chemical compound used in laboratory settings for research purposes. It functions as a selective Rho-associated protein kinase (ROCK) inhibitor. Fasudil is utilized in experimental studies to investigate various biological processes and pathways.
Lab products found in correlation
7 protocols using Fasudil
Culturing Human Pluripotent Stem Cells
Advanced Hepatogenic Differentiation Protocol
Mouse Embryonic Stem Cell Derivation
Neural Crest Cell Differentiation from iPSCs
Directed Differentiation of iPSCs
Isolation and Culture of Mouse Embryonic Fibroblasts
MEFs were cultured with DMEM/F12 (Life Technologies) supplemented with 10% fetal bovine serum (FBS, Gibco), 2 mM glutamine (Life Technologies), 0.1 mM nonessential amino acids (Life Technologies), 0.1 mM 2-mercaptoethanol (2-ME, Sigma-Aldrich), 100 U/mL penicillin, 100 mg/mL streptomycin (GE Life Science) and 10 μM fasudil (Adooq) on gelatin-coated culture plates. The medium was changed once every three days. mESCs were cultured using mES medium; KODMEM supplemented with 20% Knockout Serum Replacement (KSR; Life Technologies), 1000 U/mL leukemia inhibitory factor (LIF; Millipore), 3 μM CHIR99021 (Peprotech), 0.5 μM PD0325901 (Peprotech), 2 mM glutamine, 0.1 mM nonessential amino acids, 0.1 mM 2-ME, 100 U/mL penicillin, and 100 mg/mL streptomycin on MEF feeder cells coated plates.
Sprague-Dawley Rat Model for Fasudil Study
Male Sprague-Dawley rats (body weight, 310-340 g) were purchased from Experimental Animal Center of Sun Yat-sen University (Guangzhou, China). Animals were maintained on laboratory chow and housed in a specific pathogen-free room at a constant temperature (20-22°C) with 12 equal hours of light and dark exposure.
Fasudil was purchased from AdooQ Bioscience (Irvine, Calif) and was dissolved in 0.9% saline as a stock solution. Pentobarbital sodium was purchased from Sigma Aldrich (St. Louis, Mo).
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