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Elisa

Manufactured by Aviscera Bioscience
Sourced in United States

ELISA (Enzyme-Linked Immunosorbent Assay) is a laboratory technique used to detect and quantify specific molecules, such as proteins, in a sample. It utilizes enzyme-labeled antibodies to bind to the target analyte, and the resulting enzyme activity is measured to determine the concentration of the analyte.

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Lab products found in correlation

5 protocols using elisa

1

Serum Biomarker Assessment Protocol

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After overnight fasting, blood was collected. Serum was separated using centrifugation at 1500 × g for ten min. Serum samples were aliquoted and stored at −80 °C until analysis. High sensitivity CRP (hsCRP), total cholesterol, LDL (low-density lipoprotein), triglyceride, and HDL (high-density lipoprotein) levels were determined by Abbott Architect ci8200 autoanalyzer (Abbott Park, IL, USA). Paraoxonase activity was measured by the initial rate of paraoxon hydrolysis to yield pnitrophenol at 25 °C with spectrophotometric technique which was performed at 405 nm [16 (link)]. Fetuin-A level was evaluated by using enzyme-linked immunosorbent assay (ELISA) Aviscera Bioscience (Santa Clara, California, USA) according to the manufacturer’s instructions. Intra- and inter assay coefficients of variation were found to be 4.5%.
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2

FGF23 Quantification by ELISA

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Serum samples were analyzed for FGF23 levels using an enzyme-linked immunosorbent assay (ELISA) (Aviscera Bioscience, Santa Clara, USA). According to manufacturer’s indications, the calculated overall intra-assay coefficient of variation (CV) was between 6.0 and 8.0% and the inter-assay CV was between 8.0 and 12.0%. The minimum detectable level of FGF23 was typical at ~15 pg/mL.
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3

Serum CTRP9 Quantification by ELISA

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Serum samples were collected at baseline during a standard of care blood draw. Levels of CTRP9 were quantified by ELISA (Aviscera Bioscience) according to manufacturer’s protocol. All samples were run in duplicate. Based on previous studies, a cutoff of >81.1ng/ml, representing 2 standard deviations above the mean, was used to define elevated CTRP9 and differentiate between high and low groups.5 (link)
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4

Fasting Metabolic Biomarkers in Mice

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Mice were fasted overnight by removal to a clean cage without food at the end of their dark (feeding) cycle, approximately 6 p.m.. Mice were weighed at 8 a.m. the next morning. 30 µl blood was obtained via tail clip to assess plasma glucose (Accu-Chek Active Blood Glucose Monitoring System, Roche Diagnostics, Indianapolis, IN), plasma insulin (ELISA, Linco, Billerica, MA) and plasma CTRP9 (ELISA, Aviscera Bioscience, Santa Clara, CA). Weight and plasma measurements were recorded initially and every other week thereafter. The Homeostatic Model Assessment-Insulin Resistance (HOMA-IR), a surrogate measure of insulin resistance, was calculated initially and weekly thereafter, via HOMA calculator v2.3 (University of Oxford).
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5

Quantifying ANGPTL4 in Ovarian Cancer

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ANGPTL4 levels in ascites from ovarian cancer patients were determined by ELISA (Aviscera Bioscience, Santa Clara, CA), according to the instructions of the manufacturer. The antibody used in this kit recognizes the bioactive C-terminal processing product (cANGPTL4).
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