Prostate tissues were homogenized in phosphate buffer saline using a motorized tissue homogenizer (Talboys Engineering Corporation, Montrose, USA). The homogenate was centrifuged for 5 mins at 1,000g to remove cell debris and then the supernatant was recentrifuged at 10,000g for 10 mins. Commercially available ELISA kits were used according to the manufacturer protocols to identify prostate tissue levels of TGF-β1 (Aviscera Bioscience, USA), VEGF-A (Abcam, Cambridge, MA) and interleukin-6 (IL-6: Aviscera Bioscience, USA). Protein concentrations were measured by using bicinchoninic acid (BCA) protein assay kit (Sigma, USA) according to the manufacturer’s instructions.
Tgf β1
Manufactured by Aviscera Bioscience
Sourced in United States
TGF-β1 is a protein that plays a crucial role in the regulation of cell growth, differentiation, and proliferation. It is a member of the transforming growth factor beta superfamily and is involved in various biological processes, including immune response, tissue repair, and embryonic development.
Automatically generated - may contain errors
2 protocols using tgf β1
1
Quantification of Prostate Tissue Proteins
2
Cytokine Profiling in Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
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