EmulsiFlex-C5 homogenizer
The EmulsiFlex-C5 is a high-pressure homogenizer designed for the emulsification and dispersion of a wide range of materials. It is capable of generating pressures up to 30,000 psi (2,068 bar) to create stable emulsions and dispersions.
Lab products found in correlation
49 protocols using EmulsiFlex-C5 homogenizer
Isotopically Labeled Protein Expression and Purification
Recombinant Expression and Purification of β2-Microglobulin
1 mM IPTG overnight at 37 °C, following methods described previously.39 (link) Cells were lysed in 25 mM Tris-HCl buffer, pH
8.0 and with an Avestin Emulsiflex-C5 homogenizer. β2m is accumulated in inclusion bodies. To extract the β2m from inclusion bodies, the cell pellet was washed five times
with 25 mM Tris-HCl buffer, pH 8.0 and solubilized in 25 mM Tris-HCl,
pH 8.0 buffer containing 8 M urea, rocking overnight at room temperature.
The protein was verified to be in the soluble fraction by SDS-PAGE.
β2m was refolded by dialyzing against 25 mM Tris-HCl
buffer, pH 8.0 at 4 °C and purified by anion exchange (HiTrap
Q HP, GE Healthcare). The protein was further purified by size exclusion
chromatography with a HiLoad 26/600 Superdex 75 gel filtration column
(GE Life Sciences). Protein purity was verified by SDS-PAGE, and concentrations
for experiments were determined by measuring the absorbance at 280
nm using a molar extinction coefficient of 19,060 M–1 cm–1. [U–15N]-enriched β2m was expressed recombinantly for NMR using the same protocol
in HCDM1 minimal media supplemented with 15N-ammonium chloride.
Expression and Purification of Mutant BCL2 Proteins
Lipid-Based Methotrexate Nanoemulsion Preparation
Purification of SFTSV, HRTV, and IAV Proteins
Recombinant SARS-CoV-2 N Protein Purification
Fluorescent Protein Purification Protocol
Paclitaxel Oleate Nanoparticle Preparation
previous studies (11 (link),27 (link),28 (link)). Briefly, a lipid
mixture composed by 135 mg cholesteryl oleate (Alfa Aesar, USA), 333 mg egg
phosphatidylcholine (Lipoid, Germany), 132 mg miglyol 812N (Sasol Germany GmbH,
Germany), 6 mg cholesterol (Fabrichem, USA) and 60 mg of paclitaxel oleate
(Pharmaceuticals Co., China), was added to an aqueous phase consisting of 100 mg of
polysorbate 80 (Merck, Germany) and 10 mL Tris-HCl buffer, pH 8.05. A pre-emulsion
was obtained by ultrasonic radiation until complete solubilization of the drug.
Emulsification of all lipids, drug and aqueous phase was obtained by high-pressure
homogenization using an Emulsiflex C5 homogenizer (Avestin, Canada). After
homogenization cycles, the formed emulsion was centrifuged and the nanoparticle
sterilized by passage through 0.22 μm pore polycarbonate filter (Millipore, USA) and
kept at 4°C until used.
Recombinant fHbp Protein Expression and Purification
In brief, for protein expression,
Heterotrimeric AMPK Kinase-Dead Purification
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