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Sheep anti-Vimentin antibody

Manufactured by Affinity Biologicals
Sourced in Canada

Sheep anti-Vimentin antibody is a primary antibody that binds to the protein Vimentin, which is a type III intermediate filament found in various cell types. This antibody is used for the detection and analysis of Vimentin expression in research applications.

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2 protocols using Sheep anti-Vimentin antibody

Purified recombinant human vimentin was purchased from SinoBiological (Wayne, PA). Human fibrinogen from Calbiochem (Gibbstown, NJ), and thrombin from Sigma (St. Louis, MO). Sheep anti-Vimentin antibody was obtained from Affinity Biologicals (Ancaster, Canada), and the rabbit anti-Vimentin antibody was purchased from Proteintech (Rosemont, IL). This rabbit anti-Vimentin antibody was validated by testing its reactivity to purified recombinant vimentin and plasma vimentin by ELISA and western blot. Anti-vimentin antibody V9 and isotype immunoglobulins (IgGs) were purchased from Sigma. recombinant human vimentin rod-domain (rhRod) was expressed and purified as described29 (link)
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Purified recombinant human vimentin was purchased from SinoBiological (Wayne, PA). Human fibrinogen from Calbiochem (Gibbstown, NJ), and thrombin from Sigma (St. Louis, MO). Sheep anti-Vimentin antibody was obtained from Affinity Biologicals (Ancaster, Canada), and the rabbit anti-Vimentin antibody was purchased from Proteintech (Rosemont, IL). This rabbit anti-Vimentin antibody was validated by testing its reactivity to purified recombinant vimentin and plasma vimentin by ELISA and western blot. Anti-vimentin antibody V9 and isotype immunoglobulins (IgGs) were purchased from Sigma. recombinant human vimentin rod-domain (rhRod) was expressed and purified as described [29] .
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