Purified recombinant human vimentin was purchased from SinoBiological (Wayne, PA). Human fibrinogen from Calbiochem (Gibbstown, NJ), and thrombin from Sigma (St. Louis, MO). Sheep anti-Vimentin antibody was obtained from Affinity Biologicals (Ancaster, Canada), and the rabbit anti-Vimentin antibody was purchased from Proteintech (Rosemont, IL). This rabbit anti-Vimentin antibody was validated by testing its reactivity to purified recombinant vimentin and plasma vimentin by ELISA and western blot. Anti-vimentin antibody V9 and isotype immunoglobulins (IgGs) were purchased from Sigma. recombinant human vimentin rod-domain (rhRod) was expressed and purified as described29 (link)
Isotype immunoglobulins iggs
Manufactured by Merck Group
Isotype immunoglobulins (IgGs) are a class of antibody molecules produced by the immune system. They play a crucial role in the body's defense against foreign pathogens. IgGs are the most abundant type of antibody found in the blood and other bodily fluids.
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Lab products found in correlation
2 protocols using isotype immunoglobulins iggs
1
Recombinant Vimentin Purification and Validation
2
Purification and Validation of Vimentin
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Purified recombinant human vimentin was purchased from SinoBiological (Wayne, PA). Human fibrinogen from Calbiochem (Gibbstown, NJ), and thrombin from Sigma (St. Louis, MO). Sheep anti-Vimentin antibody was obtained from Affinity Biologicals (Ancaster, Canada), and the rabbit anti-Vimentin antibody was purchased from Proteintech (Rosemont, IL). This rabbit anti-Vimentin antibody was validated by testing its reactivity to purified recombinant vimentin and plasma vimentin by ELISA and western blot. Anti-vimentin antibody V9 and isotype immunoglobulins (IgGs) were purchased from Sigma. recombinant human vimentin rod-domain (rhRod) was expressed and purified as described [29] .
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