10nm gold particles
10nm gold particles are a type of laboratory equipment used for various scientific applications. These particles have a diameter of 10 nanometers and are composed of the metallic element gold. They are commonly used in fields such as nanotechnology, biotechnology, and materials science for their unique optical and physical properties.
Lab products found in correlation
10 protocols using 10nm gold particles
Immunogold Labeling of Exosomes
Immunogold Labeling of Vimentin in Vero E6 Cells
Immunogold Labeling of PD-L1 in TEM
Immunogold Labeling of Tau Proteins
Immunogold Labeling for Electron Microscopy
carbon/formvar coated grids and allowed to absorb to the formvar for a minimum
of 1 minute. For immunogold staining the grids were placed into a blocking
buffer for a block/permeabilization step for 1 hour. Without rinsing, the grids
were immediately placed into the primary antibody at the appropriate dilution
overnight at 4°C (monoclonal anti-CD9 1:10, Abcam). As controls, some
grids were not exposed to the primary antibody. The next day, all of the grids
were rinsed with PBS then floated on drops of the appropriate secondary antibody
attached with 10nm gold particles (AURION, Hatfield, PA) for 2 hours at room
temperature. Grids were rinsed with PBS and were placed in 2.5%
Glutaraldehyde in 0.1M phosphate buffer for 15 minutes. After rinsing in PBS and
distilled water the grids were allowed to dry and stained for contrast using
uranyl acetate. The samples were viewed with a Tecnai Bio Twin transmission
electron microscope (FEI, Hillsboro, OR) and images were taken with an AMT CCD
Camera (Advanced Microscopy Techniques, Danvers, MA).
Immunogold Labeling of CD151
Immunogold Labeling of Exosomes
Immunogold Labeling of Exosomes for TEM Analysis
electron microscopy (TEM) characterization was performed using a JEOL
JEM-2100F field emission S/TEM equipped with an Oxford X-MaxN 80 mm2
SDD detector for elemental analysis. TEM images were acquired at a
200 kV accelerating voltage and 116 μA emission current. DigitalMicrograph
GMS3 (Gatan) and Aztec TEM (Oxford Instruments) software packages
were used for the TEM data and energy dispersive X-ray (EDX) spectral
analysis and interpretation, respectively. Briefly, for the immunogold
labeling with antibodies, GPC-1 antibodies (pa5–51290, Thermo
Scientific) were attached on 10 nm gold particles (AURION) according
to the manufacturer’s instructions at room temperature. Healthy
plasma and PDAC plasma sample pools were prepared. 200 μL from
each of 5 healthy or 5 PDAC plasma samples was taken to form a healthy
plasma or PDAC plasma sample pool. Exosomes were isolated as previously
described. Then isolated exosome samples were conjugated with the
gold-particles attached GPC-1 antibodies at the appropriate dilution
overnight at 4 °C. Samples for TEM characterization were drop-cast
from dilute aqueous suspensions onto amorphous carbon coated (200
nm) Cu grids (Agar Scientific) and dried naturally overnight.
Immunogold Labeling for Electron Microscopy
Immunogold Labeling of PD-L1 in Specimens
After rinsing in PBS and distilled water the grids were allowed to dry and stained for contrast using uranyl acetate. The samples were viewed with a Tecnai Bio Twin transmission electron microscope (FEI, Hillsboro, OR) and images were taken with an AMT CCD Camera (Advanced Microscopy Techniques, Danvers, MA).
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