Streptomycin sulfate

Manufactured by Research Products International

Sourced in Israel

Streptomycin sulfate is a white, crystalline, water-soluble powder. It is a broad-spectrum antibiotic derived from the bacterium Streptomyces griseus. Streptomycin sulfate inhibits bacterial protein synthesis, thereby disrupting bacterial growth and replication.

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Lab products found in correlation

Fluconazole, by Merck Group (1 mentions) Penicillin g, by Merck Group (1 mentions) Sodium chloride (nacl), by Americanbio (1 mentions) Soytone, by Teknova (1 mentions) Hy soy, by Teknova (1 mentions) Guanine, by Merck Group (1 mentions)

2 protocols using streptomycin sulfate

Fungal Dissemination Induction Protocol

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Mice were treated for five days with 0.5 mg/ml fluconazole (Sigma), 1 mg/ml Penicillin G (Sigma), and 2 mg/ml streptomycin sulfate (Research Products International) ad libitum in the drinking water. fluconazole was withdrawn and mice were orally gavaged with 1×10⁸C. albicans cells three times spaced across five days, followed by i.p. injection of 400μg C.alb-IgG, S.cer-IgG, or sterile PBS (Figure S4B). Immunosuppression and systemic fungal dissemination were induced by i.p. injections of 150mg/kg mouse weight of cyclophosphamide (Cyc; R&D Systems) on days 0, 2, and 4, followed by a second series of injections on days 14, 16, and 18. Weight was measured, and moribund mice were sacrificed for serum, liver, and kidney tissue collection.

Doron I., Leonardi I., Li X.V., Fiers W.D., Semon A., Bialt-DeCelie M., Migaud M., Gao I.H., Lin W.Y., Kusakabe T., Puel A, & Iliev I.D. (2021). Human gut mycobiota tune immunity via CARD9-dependent induction of anti-fungal IgG antibodies. Cell, 184(4), 1017-1031.e14.

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Cultivation of Bacterial Strains

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Bacterial strains used in this study are shown in Table 1. All bacterial strains were maintained in animal-product-free Hy-Soy (HS) medium (10 g/L Soytone [Teknova, Hollister, CA], 5 g/L Hy-yest [Kerry Bio-Science, Beloit, WI] and 5 g/L sodium chloride [American Bio, Natick, MA]) at 37°C. When needed, agar (Sigma–Aldrich, St. Louis, MO) was added at 15 g/L. For CVD 1926, medium was supplemented with guanine (0.005% weight/volume [w/v] final concentration; Sigma–Aldrich). For S. Typhimurium SL1344, medium was supplemented with 50 μg/mL streptomycin sulfate (Research Products International, Mt. Prospect, IL).

Allen J.C., Toapanta F.R., Baliban S.M., Sztein M.B, & Tennant S.M. (2023). Reduced immunogenicity of a live Salmonella enterica serovar Typhimurium vaccine in aged mice. Frontiers in Immunology, 14, 1190339.

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