In all images, the color tables were adapted for optimal contrast. Background subtraction was usually below 5% of the maximum signal intensity.
Imspector software
Imspector software is a data processing and analysis tool developed by Abberior. It is designed to handle and analyze data acquired from microscopy systems. The software provides functionalities for data import, visualization, and processing, enabling users to extract relevant information from microscopy images and datasets.
Lab products found in correlation
25 protocols using Imspector software
Image Deconvolution and Contrast Optimization
In all images, the color tables were adapted for optimal contrast. Background subtraction was usually below 5% of the maximum signal intensity.
STED-FCS Microscopy on Biosafety Level 3
Nanobody Staining for STED Imaging
STED Microscopy of Nucleoids in MEFs
Super-Resolution Microscopy and FCS Protocols
Confocal microscope FCS measurements were performed on a Zeiss LSM 780 (Zeiss, Iena, Germany) using a HeNe 633-nm laser as the excitation source. Fluorescence excitation and collection were performed using a 63×/1.40-NA PlanApo oil immersion objective (Zeiss). Acquisition operations were controlled by the Zeiss Zen software. Point FCS was used to calibrate the observation spot with the LSM 780 internal hardware correlator.
STED Imaging of Differentiated pHBE Cells
STED Imaging with Deconvolution and Filtering
High-Resolution STED Microscopy of Cells
STED Imaging with Deconvolution and Filtering
Deconvolution of STED images was performed in Imspector software (Abberior Instruments) via the linear deconvolution tool.
Images were further processed in Fiji‐Imagej (Schindelin et al,
Deconvolution and Colocalization Analysis
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