AMT CCD Camera
The AMT CCD Camera is a high-performance digital camera designed for advanced microscopy applications. It features a charge-coupled device (CCD) sensor that captures high-resolution images with excellent sensitivity and low noise levels. The camera is capable of capturing detailed images of microscopic samples and is suitable for a wide range of scientific and research applications.
Lab products found in correlation
17 protocols using AMT CCD Camera
Extracellular Vesicle Isolation and Characterization
Immunogold Labeling of PD-L1 in TEM
Immunogold Labeling for Transmission Electron Microscopy
Ultrastructural Analysis of Ventricular Tissue
Immunogold Labeling of Alveolospheres
Transmission Electron Microscopy Specimen Prep
Immunogold Labeling for Electron Microscopy
carbon/formvar coated grids and allowed to absorb to the formvar for a minimum
of 1 minute. For immunogold staining the grids were placed into a blocking
buffer for a block/permeabilization step for 1 hour. Without rinsing, the grids
were immediately placed into the primary antibody at the appropriate dilution
overnight at 4°C (monoclonal anti-CD9 1:10, Abcam). As controls, some
grids were not exposed to the primary antibody. The next day, all of the grids
were rinsed with PBS then floated on drops of the appropriate secondary antibody
attached with 10nm gold particles (AURION, Hatfield, PA) for 2 hours at room
temperature. Grids were rinsed with PBS and were placed in 2.5%
Glutaraldehyde in 0.1M phosphate buffer for 15 minutes. After rinsing in PBS and
distilled water the grids were allowed to dry and stained for contrast using
uranyl acetate. The samples were viewed with a Tecnai Bio Twin transmission
electron microscope (FEI, Hillsboro, OR) and images were taken with an AMT CCD
Camera (Advanced Microscopy Techniques, Danvers, MA).
Exosome Immunogold Labeling and TEM Imaging
Ultrastructural Examination of Neural Tissue
Isolation and Characterization of Exosomes
For electron microscopy, exosomes were fixed with 2% PFA and loaded on Formvar and carbon-coated copper grids. Then the grids were placed on 2% gelatin at 37°C for 20 min, rinsed with 0.15 M glycine and PBS, and the sections were blocked using 1% cold water fish-skin gelatin. Grids were viewed with an FEI Tecnai G2 spirit transmission electron microscope (FEI) and photographed using an AMT CCD camera (Advanced Microscopy Techniques).
Exosome particle size and concentration was determined using NTA with ZetaView PMX 120 (Particle Metrix).
To track exosomes, they were labeled with fluorescent dye using the PKH67 fluorescent cell linker kit (Sigma-Aldrich), according to the manufacturer’s instructions. After labeling, the exosomes were washed in PBS and collected by ultracentrifugation (100,000 × g for 20 min) at 4°C. Then, PKH67-labeled exosomes were resuspended in PBS.
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