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Amt ccd camera

Manufactured by Advanced Microscopy Techniques
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Sourced in United States, China

The AMT CCD Camera is a high-performance digital camera designed for advanced microscopy applications. It features a charge-coupled device (CCD) sensor that captures high-resolution images with excellent sensitivity and low noise levels. The camera is capable of capturing detailed images of microscopic samples and is suitable for a wide range of scientific and research applications.

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Lab products found in correlation

10nm gold particles, by Aurion (4 mentions) Tecnai bio twin transmission electron microscope, by Thermo Fisher Scientific (4 mentions) H 7650, by Hitachi (2 mentions) Monoclonal anti cd9, by Abcam (2 mentions) Sw41 swinging bucket rotor, by Beckman Coulter (1 mentions) H 7650 tem, by Hitachi (1 mentions) Eagle ccd camera, by Thermo Fisher Scientific (1 mentions) Vitrobot plunge freezer, by Thermo Fisher Scientific (1 mentions) T20 microscope, by Thermo Fisher Scientific (1 mentions) Anti gpc1pipa528055, by Thermo Fisher Scientific (1 mentions) Anti cd9 ab92726, by Abcam (1 mentions) Polyvinylpyrrolidone pvp, by Merck Group (1 mentions) Hepes, by Merck Group (1 mentions) Methylcellulose, by Merck Group (1 mentions) L lysine, by Merck Group (1 mentions) Tecnai g2 spirit transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Zetaview pmx 120, by Particle Metrix (1 mentions) Pkh67 fluorescent cell linker kit, by Merck Group (1 mentions) Tecnai g2 12 bio twin, by Thermo Fisher Scientific (1 mentions) Tecnai biotwin tem, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CCD camera (13 citations) AMT 2k CCD camera (9 citations) AMT XR41 digital CCD camera (7 citations) AMT Advantage 10 CCD Camera System (3 citations) AMT XR 60 CCD camera system (2 citations) AMT XR-60 CCD Digital Camera System (2 citations)

18 protocols using amt ccd camera

1

Extracellular Vesicle Isolation and Characterization

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Extracellular vesicle (including exosomes and microvesicles) preparation and examination were performed according to previous description (Jung and Mun, 2018 ). LMH cells were cultured to near confluency in a 75 cm dish, and cells were mock infected or infected at a MOI of 1 in serum-free DMEM at 4 °C for 1 h. Then cells were washed once with PBS and cultured in extracellular vesicle-free full DMEM medium in the absence or in the present of PP1 or PP2. Medium was harvested after 24 hpi and extracellular vesicles were purified by differential centrifugation at 4 °C (300×g for 10 min, 2000×g for 20 min, 10000×g for 40 min using a Beckman JA-25.15 fixed angle rotor, then ultracentrifuged 100000×g for 90 min using a Beckman SW-41 swinging bucket rotor). The extracellular vesicle pellets generated were resuspended in serum-free DMEM, fixed and dried. Then samples were coated with gold/palladium alloy by sputter coating and examined under a Hitachi H-7650 transmission electron microscope (Hitachi High Technologies, Shanghai, China), and images were taken using an AMT CCD camera (Advanced Microscopy Techniques, Woburn, MA).
Wang Z., Sun B., Gao Q., Ma Y., Liang Y., Chen Z., Wu H., Cui L., Shao Y., Wei P., Li H, & Liu S. (2019). Host Src controls gallid alpha herpesvirus 1 intercellular spread in a cellular fatty acid metabolism-dependent manner. Virology, 537, 1-13.
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2

Negative Staining and Cryo-EM Imaging

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For negative staining, samples were absorbed onto freshly glow-discharged carbon-coated grids, rinsed with water, and stained with 2% uranyl acetate. Specimens were examined on a Hitachi H-7650 TEM (transmission electron microscopy) (Hitachi High Technologies America, Inc., Clarksburg, MD, USA) at 80 kV and images were recorded using an AMT CCD camera (Advanced Microscopy Techniques, Corp., Woburn, MA, USA).
For cryo-EM, 4 μL of sample was blotted onto freshly glow-discharged holey carbon grids (Quantifoil R2/2, SPI, West Chester, PA, USA) and vitrified in a Vitrobot plunge freezer (FEI, Hillsboro, OR, USA). Images were recorded with a T20 microscope (FEI) at 200 kV on an Eagle CCD camera (FEI).
Sine J., Urban C., Thayer D., Charron H., Valim N., Tata D.B., Schiff R., Blumenthal R., Joshi A, & Puri A. (2014). Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts. International Journal of Nanomedicine, 10, 125-145.
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3

Immunogold Labeling for Transmission Electron Microscopy

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Fixed specimens at an optimal concentration were placed onto a 400 mesh
carbon/formvar coated grids and allowed to absorb to the formvar for a minimum
of 1 minute. For immunogold staining the grids were placed into a blocking
buffer for a block/permeabilization step for 1 hr. Without rinsing, the grids
were immediately placed into the primary antibody at the appropriate dilution
overnight at 4°C (1:300 anti-CD9 ab92726, Abcam and anti-GPC1
PIPA528055, Thermo Scientific). As controls, some of the grids were not exposed
to the primary antibody. The following day, all the grids were rinsed with PBS
then floated on drops of the appropriate secondary antibody attached with 10 nm
gold particles (AURION, Hatfield, PA) for 2 hrs at room temperature. Grids were
rinsed with PBS and were placed in 2.5% Glutaraldehyde in 0.1M Phosphate
buffer for 15 min. After rinsing in PBS and distilled water the grids were
allowed to dry and stained for contrast using uranyl acetate. The samples were
viewed with a Tecnai Bio Twin transmission electron microscope (FEI, Hillsboro,
OR) and images were taken with an AMT CCD Camera (Advanced Microscopy
Techniques, Danvers, MA).
Melo S.A., Luecke L.B., Kahlert C., Fernandez A.F., Gammon S.T., Kaye J., LeBleu V.S., Mittendorf E.A., Weitz J., Rahbari N., Reissfelder C., Pilarsky C., Fraga M.F., Piwnica-Worms D, & Kalluri R. (2015). Glypican1 identifies cancer exosomes and facilitates early detection of cancer. Nature, 523(7559), 177-182.
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4

Ultrastructural Analysis of Ventricular Tissue

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Ventricular specimens (cubes less than 3 mm square) were fixed in 2.5% glutaraldehyde, underwent sectioning and heavy metal uranyl acetate staining for contrast by the Electron Microscopy Center of Indiana University School of Medicine. At least 6 separate sections from each animal strain were analyzed. Electron micrographs were acquired on a transmission electron microscope, Tecnai BioTwin (FEI) equipped with AMT CCD Camera (Advanced Microscopy Techniques).
Shi J., Surma M, & Wei L. (2018). Disruption of ROCK1 gene restores autophagic flux and mitigates doxorubicin-induced cardiotoxicity. Oncotarget, 9(16), 12995-13008.
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5

Immunogold Labeling of Alveolospheres

Cited 1 time
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Alveolospheres were processed for immunogold labeling described previously (Ridsdale et al., 2011 (link)). Alveolospheres were first fixed in situ with 4% paraformaldehyde (EMS), 0.1% glularaldehyde (EMS), 75 mM L-lysine (Sigma), 10 mM INaO4 (Sigma), and 0.1% CaCl2 in 0.2M HEPES (Sigma), pH 7.2 at room temperature for 10 min, followed by postfixation with fresh fixative at 4 °C overnight. They were embedded with 10% gelatin, cryoprotected with 2.3M Polyvinylpyrrolidone (PVP; M.W. 10,000; Sigma)/sucrose (Sigma) in 0.2M HEPES, pH 7.2, and frozen in liquid nitrogen for cryoultramicrotomy. 70 to 80 nm frozen sections were picked up with mixture of 1.15M PVP/sucrose, 1% methyl cellulose (Sigma), 0.2% uranyl acetate (EMS), and 0.1% glutatraldehyde, transferred to 200 mesh Butvar® coated nickel grids (EMS), and stored at −20 °C until they were ready for immunogold labeling. To localize SFTPB or SFTPC proteins, thawed frozen sections were stained with rabbit polyclonal Ab directed against mature SFTPB (Seven Hills; Lin et al., 1996 (link)) or mature SFTPC (Seven Hills; Ross et al., 1999 (link)), and 10 nm protein A gold (CMC). Electron micrographs of labeled cells were acquired using a Hitachi TEM 7650 (Hitachi High Technologies America) with an AMT CCD camera (Advanced Microscopy Techniques).
Jacob A., Morley M., Hawkins F., McCauley K.B., Jean J.C., Heins H., Na C.L., Weaver T.E., Vedaie M., Hurley K., Hinds A., Russo S.J., Kook S., Zacharias W., Ochs M., Traber K., Quinton L.J., Crane A., Davis B.R., White F.V., Wambach J., Whitsett J.A., Cole F.S., Morrisey E.E., Guttentag S.H., Beers M.F, & Kotton D.N. (2017). Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells. Cell stem cell, 21(4), 472-488.e10.
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6

Isolation and Characterization of Exosomes

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After 72-hr macrophage culture, debris and dead cells in the medium were removed by centrifugation at 1,000 × g for 10 min and then filtered through a 0.2-mm filter. The medium was then subjected to ultracentrifugation at 100,000 × g for 4–6 hr at 4°C. After washing with PBS (100,000 × g for 20 min), the Exo-containing pellet was resuspended in PBS.
For electron microscopy, exosomes were fixed with 2% PFA and loaded on Formvar and carbon-coated copper grids. Then the grids were placed on 2% gelatin at 37°C for 20 min, rinsed with 0.15 M glycine and PBS, and the sections were blocked using 1% cold water fish-skin gelatin. Grids were viewed with an FEI Tecnai G2 spirit transmission electron microscope (FEI) and photographed using an AMT CCD camera (Advanced Microscopy Techniques).
Exosome particle size and concentration was determined using NTA with ZetaView PMX 120 (Particle Metrix).
To track exosomes, they were labeled with fluorescent dye using the PKH67 fluorescent cell linker kit (Sigma-Aldrich), according to the manufacturer’s instructions. After labeling, the exosomes were washed in PBS and collected by ultracentrifugation (100,000 × g for 20 min) at 4°C. Then, PKH67-labeled exosomes were resuspended in PBS.
Cui H., He Y., Chen S., Zhang D., Yu Y, & Fan C. (2018). Macrophage-Derived miRNA-Containing Exosomes Induce Peritendinous Fibrosis after Tendon Injury through the miR-21-5p/Smad7 Pathway. Molecular Therapy. Nucleic Acids, 14, 114-130.
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7

Exosome Analysis by Transmission Electron Microscopy

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Exosomes from patients' plasma were fixed at optimal concentration and settled on a 400-mesh carbon/formvar covered grids. They were supposed to absorb the formvar for at least 1 min. The samples were viewed with the Tecnai Bio Twin transmission electron microscope (FEI) and images were obtained with an AMT CCD Camera (Advanced Microscopy Techniques).
Zhang C., Fan Y., Che X., Zhang M., Li Z., Li C., Wang S., Wen T., Hou K., Shao X., Liu Y, & Qu X. (2020). Anti-PD-1 Therapy Response Predicted by the Combination of Exosomal PD-L1 and CD28. Frontiers in Oncology, 10, 760.
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8

Exosome Characterization by TEM

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Exosome samples were fixed with 1% glutaraldehyde in PBS at an optimal concentration. The mixture was then spotted onto a 300 mesh carbon/formvar-coated grids and dried at room temperature. Next, the grids were washed with PBS and stained for contrast using uranyl acetate in water for 10 min. Subsequent to staining, samples were imaged by TEM (FEI, Hillsboro, OR, USA), and images were captured with an AMT CCD Camera (Advanced Microscopy Techniques, Danvers, MA, USA).
He L., Zhu D., Wang J, & Wu X. (2018). A highly efficient method for isolating urinary exosomes. International Journal of Molecular Medicine, 43(1), 83-90.
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9

Transmission Electron Microscopy Specimen Prep

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Fixed specimens at an optimal concentration were placed onto 400-mesh carbon/formvar coated grids and allowed to absorb to the formvar for a minimum of 1 min. After rinsing in 1x PBS and distilled water the grids were allowed to dry and stained for contrast using uranyl acetate. The samples were viewed with a Tecnai Bio Twin transmission electron microscope (TEI) and images were taken with an AMT CCD Camera (Advanced Microscopy Techniques, Woburn, MA, USA).
Rahbari M., Pecqueux M., Aust D., Stephan H., Tiebel O., Chatzigeorgiou A., Tonn T., Baenke F., Rao V., Ziegler N., Greif H., Lin K., Weitz J., Rahbari N.N, & Kahlert C. (2019). Expression of Glypican 3 Is an Independent Prognostic Biomarker in Primary Gastro-Esophageal Adenocarcinoma and Corresponding Serum Exosomes. Journal of Clinical Medicine, 8(5), 696.
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10

Transmission Electron Microscopy Analysis

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Samples were sent to Plague Diagnose and Technical Service Center of Harbin Veterinary Research Institute for transmission electron microscopy analysis. Briefly, cell samples were coated with gold/palladium alloy by sputter coating at 3 or 24 hpi and examined under a Hitachi H-7650 transmission electron microscope (Hitachi High Technologies, Shanghai, China), and images were taken using an AMT CCD camera (Advanced Microscopy Techniques, Woburn, MA).
Qiao Y., Wang Z., Han Z., Shao Y., Ma Y., Liang Y., Chen Z., Wu H., Cui L., Zhang Y., Liu S, & Li H. (2020). Global exploration of the metabolic requirements of gallid alphaherpesvirus 1. PLoS Pathogens, 16(8), e1008815.
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