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Formvar-Insulated Nichrome wires

Manufactured by A-M Systems
Sourced in United States

Formvar-Insulated Nichrome wires are a type of electrical wire featuring a Formvar insulation layer over a Nichrome core. Nichrome is an alloy composed of nickel and chromium, known for its high resistance and durability. The Formvar insulation provides electrical isolation and protection for the wire. This product is suitable for use in various laboratory and industrial applications that require a reliable and insulated electrical conductor.

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19 protocols using Formvar-Insulated Nichrome wires

Microelectrodes consisted of a drivable bundle of sixteen 25.4 µm diameter Formvar-Insulated Nichrome wires (761500, A-M Systems, Carlsborg, WA) within a 27-gauge cannula (B000FN3M7K, Amazon Supply) and two 127 µm diameter PFA-coated, annealed strength stainless-steel ground wires (791400, A-M Systems, Carlsborg, WA). All wires were electrically connected to a nano-strip omnetics connector (A79042-001, Omnetics Connector Corp., Minneapolis, MN) on a custom 24-contact, individually routed and gold immersed circuit board (San Francisco Circuits, San Mateo, CA).
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Microelectrodes consisted of a drivable bundle of sixteen 25.4 µm diameter Formvar-Insulated Nichrome wires (761500, A-M Systems, Carlsborg, WA) within a 27-gauge cannula (B000FN3M7K, Amazon Supply) and two 127 µm diameter PFA-coated, annealed strength stainless-steel ground wires (791400, A-M Systems, Carlsborg, WA). All wires were electrically connected to a nano-strip omnetics connector (A79042-001, Omnetics Connector Corp., Minneapolis, MN) on a custom 24-contact, individually routed and gold immersed circuit board (San Francisco Circuits, San Mateo, CA). Sixteen individual recording wires were soldered to individual channels of an Omnetics connector. The sixteen wire bundle was integrated into a microdrive permitting advancement in ~ 42 μm increments.
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Rats were anesthetized with isoflurane (3% induction, 1–2% maintenance in 2 L/m O2) and placed in a standard stereotaxic device (Kopf Instruments, Tujunga, CA). Two electrode bundles consisting of 16 individual 25 μm formvar insulated nichrome wires (A-M systems, Carlsborg, WA) were implanted. The bundles were each contained in 27 gauge cannulae and mounted in a custom-built 3d printed microdrive. The center of each bundle was spaced 800 μm apart and aligned on the A-P axis. Prior to surgery, electrode bundles were trimmed to 1–2 mm and spread to allow at least 25 μm space between each wire. Arrays were centered unilaterally above right ACC: AP +2.7 mm; ML +0.7 mm and lowered 1.0 mm from brain surface. Headcaps were secured with 0–80 1/8” machine screws and dental acrylic and encased in a custom 3d printed protective cover. Rats received 5 mg/kg carprofen s.c. on the day of surgery and four days following. 60 mg/kg p.o. cephalexin was administered for ten days following surgery to prevent infection. Electrodes were slowly advanced to final recording coordinates during surgical recovery.
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Microelectrodes were constructed on site and consisted of a drivable bundle of sixteen Formvar-Insulated Nichrome wires (25.4 µm diameter: 761500, A-M Systems, Carlsborg, WA) within a 27-gauge cannula (B000FN3M7K, Amazon Supply). The cannula bundle was attached to a manually operated microdrive calibrated to permit ~0.042 mm advancement increments. Two free-hanging 127 µm diameter PFA-coated stainless-steel ground wires were also part of the assembly (791400, A-M Systems, Carlsborg, WA). All wires were electrically connected to a Nano Strip omnetics connector (A79042-001, Omnetics Connector Corp., Minneapolis, MN) on a custom 24-contact, individually-routed and gold-immersed circuit board (San Francisco Circuits, San Mateo, CA).
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Microelectrodes consisted of a drivable bundle of sixteen 25.4 μm diameter Formvar-Insulated Nichrome wires (761500, A-M Systems, Carlsborg, WA) within a 27-gauge cannula (B000FN3M7K, Amazon Supply) and two 127 μm diameter PFA-coated, annealed strength stainless-steel ground wires (791400, A-M Systems, Carlsborg, WA). All wires were electrically connected to a nano-strip Omnetics connector (A79042-001, Omnetics Connector Corp., Minneapolis, MN) on a custom 24-contact, individually routed and gold immersed circuit board (San Francisco Circuits, San Mateo, CA). Sixteen individual recording wires were soldered to individual channels of an Omnetics connector. The sixteen wire bundle was integrated into a microdrive permitting advancement in ~40 μm increments.
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6

Chronic VTA Electrode Implantation and Hippocampal Lesion

All surgical procedures adhered to guidelines for aseptic technique. For electrode implantation, a drivable bundle of eight 25-um diameter formvar insulated nichrome wires (A-M systems, Carlsborg, WA) chronically implanted dorsal to VTA in the left or right hemisphere at 5.3 mm posterior to bregma, 0.7 mm laterally, and 7.5 mm ventral to the brain surface at an angle of 5° toward the midline from vertical. Some rats (n = 9) also received neurotoxic lesion of ipsilateral hippocampus by the infusion of NMDA (20 mg/ml) at seven sites in each hemisphere (see Fig. S1 for the surgical coordinates). Controls (n = 5) received sham lesions in which burr holes were drilled and the pipette tip lowered into the brain but no solution delivered. Cephalexin (15 mg/kg p.o.) was administered twice daily for two weeks post-operatively.
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7

Chronic VTA Electrode Implantation and Hippocampal Lesion

All surgical procedures adhered to guidelines for aseptic technique. For electrode implantation, a drivable bundle of eight 25-um diameter formvar insulated nichrome wires (A-M systems, Carlsborg, WA) chronically implanted dorsal to VTA in the left or right hemisphere at 5.3 mm posterior to bregma, 0.7 mm laterally, and 7.5 mm ventral to the brain surface at an angle of 5° toward the midline from vertical. Some rats (n = 9) also received neurotoxic lesion of ipsilateral hippocampus by the infusion of NMDA (20 mg/ml) at seven sites in each hemisphere (see Fig. S1 for the surgical coordinates). Controls (n = 5) received sham lesions in which burr holes were drilled and the pipette tip lowered into the brain but no solution delivered. Cephalexin (15 mg/kg p.o.) was administered twice daily for two weeks post-operatively.
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Three bare silver wires (127 μm diameter; A-M Systems, Inc., Carlsborg, WA, USA) and two formvar-insulated nichrome wires (66 μm diameter; A-M Systems, Inc., Carlsborg, WA, USA) were soldered into separate channels of an eight-channel headstage (Plexon Inc., Dallas, TX, USA). Two silver wires were used as recording electrodes, while the other one was used as reference electrode. The bipolar electrode was made by twisting the nichrome wires around each other.
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Microelectrodes consisted of a drivable bundle of sixteen 25.4 µm diameter Formvar-Insulated Nichrome wires (761500, A-M Systems, Carlsborg, WA) within a 27-gauge cannula (B000FN3M7K, Amazon Supply) and two 127 µm diameter PFA-coated, annealed strength stainless-steel ground wires (791400, A-M Systems, Carlsborg, WA). All wires were electrically connected to a nano-strip Omnetics connector (A79042-001, Omnetics Connector Corp., Minneapolis, MN) on a custom 24-contact, individually routed and gold immersed circuit board (San Francisco Circuits, San Mateo, CA). Sixteen individual recording wires were soldered to individual channels of an Omnetics connector. The sixteen wire bundle was integrated into a microdrive permitting advancement in ~42 μm increments.
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Microelectrodes consisted of a drivable bundle of sixteen 25.4 µm diameter Formvar-Insulated Nichrome wires (761500, A-M Systems, Carlsborg, WA) within a 27-gauge cannula (B000FN3M7K, Amazon Supply) and two 127 µm diameter PFA-coated, annealed strength stainless-steel ground wires (791400, A-M Systems, Carlsborg, WA). All wires were electrically connected to a nano-strip Omnetics connector (A79042-001, Omnetics Connector Corp., Minneapolis, MN) on a custom 24-contact, individually routed and gold immersed circuit board (San Francisco Circuits, San Mateo, CA). Sixteen individual recording wires were soldered to individual channels of an Omnetics connector. The sixteen wire bundle was integrated into a microdrive permitting advancement in ~42 μm increments.
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