Penicillin streptomycin

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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

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33 948 protocols using penicillin streptomycin

Cell Culture Conditions for Diverse Species

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CHM13 cells were cultured in complete AmnioMax C-100 Basal Medium (Thermo Fisher Scientific, 17001082) supplemented with 15% AmnioMax C-100 Supplement (Thermo Fisher Scientific, 12556015) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). HG00733 cells were cultured in RPMI 1640 with l-glutamine (Thermo Fisher Scientific, 11875093) supplemented with 15% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). Chimpanzee (P. troglodytes; S006007) and macaque (M. mulatta; AG07107) cells were cultured in MEMα containing ribonucleosides, deoxyribonucleosides and l-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 12% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). Orangutan (P. abelii; PR01109) cells were cultured in MEMα containing ribonucleosides, deoxyribonucleosides and l-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 15% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). All cells were cultured in a humidity-controlled environment at 37 °C with 5% CO₂.

Logsdon G.A., Vollger M.R., Hsieh P., Mao Y., Liskovykh M.A., Koren S., Nurk S., Mercuri L., Dishuck P.C., Rhie A., de Lima L.G., Dvorkina T., Porubsky D., Harvey W.T., Mikheenko A., Bzikadze A.V., Kremitzki M., Graves-Lindsay T.A., Jain C., Hoekzema K., Murali S.C., Munson K.M., Baker C., Sorensen M., Lewis A.M., Surti U., Gerton J.L., Larionov V., Ventura M., Miga K.H., Phillippy A.M, & Eichler E.E. (2021). The structure, function and evolution of a complete human chromosome 8. Nature, 593(7857), 101-107.

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Cell Culture Conditions for Human and Primate Cells

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CHM1 cells were cultured in complete AmnioMax C-100 Basal Medium (Thermo Fisher Scientific, 17001082) supplemented with 15% AmnioMax C-100 Supplement (Thermo Fisher Scientific, 12556015) and 1% penicillin–streptomycin (Thermo Fisher Scientific, 15140122). HG00733 (Homo sapiens) cells were cultured in RPMI-1650 medium (Sigma-Aldrich, R8758) supplemented with 15% fetal bovine serum (FBS; Thermo Fisher Scientific, 16000-044) and 1% penicillin–streptomycin (Thermo Fisher Scientific, 15140122). Chimpanzee (P. troglodytes; Clint; S006007) and macaque (Macaque mulatta; AG07107) cells were cultured in MEM α containing ribonucleosides, deoxyribonucleosides and l-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 12% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin–streptomycin (Thermo Fisher Scientific, 15140122). Orangutan (P. abelii; Susie; PR01109) cells were cultured in MEM α containing ribonucleosides, deoxyribonucleosides and l-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 15% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin–streptomycin (Thermo Fisher Scientific, 15140122). All cells were cultured in a humidity-controlled environment at 37 °C under 95% O₂.

Logsdon G.A., Rozanski A.N., Ryabov F., Potapova T., Shepelev V.A., Catacchio C.R., Porubsky D., Mao Y., Yoo D., Rautiainen M., Koren S., Nurk S., Lucas J.K., Hoekzema K., Munson K.M., Gerton J.L., Phillippy A.M., Ventura M., Alexandrov I.A, & Eichler E.E. (2024). The variation and evolution of complete human centromeres. Nature, 629(8010), 136-145.

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Cell Line Maintenance Protocols

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All cell lines (CFPAC-1, NCI-H441, RPMI-6666, COS-7, T2), except for T2A3 cells (a kind gift from Eric Lutz and Elizabeth Jaffee, Johns Hopkins University, Baltimore, MD) that were procured from ATCC. CFPAC-1 and NCI-H441 cell lines were maintained in McCoy’s 5A (Modified) media (Thermo Fisher Scientific) supplemented with 10% FBS (HyClone Defined, GE Healthcare) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific). The COS-7 cell line was maintained in Dulbecco’s modified Eagle’s medium (high glucose, pyruvate) media (Thermo Fisher Scientific) supplemented with 10% FBS (HyClone Defined, GE Healthcare) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific). T2 and T2A3 cells were maintained in RPMI-1640 (ATCC) supplemented with 10% FBS (HyClone Defined, GE Healthcare) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific). The RPMI-6666 cell line was maintained in RPMI-1640 (ATCC) supplemented with 20% FBS (HyClone Defined, GE Healthcare) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific). All cells, including primary human T cells, were maintained at 37 °C under 5% CO₂.

Hwang M.S., Mog B.J., Douglass J., Pearlman A.H., Hsiue E.H., Paul S., DiNapoli S.R., Konig M.F., Pardoll D.M., Gabelli S.B., Bettegowda C., Papadopoulos N., Vogelstein B., Zhou S, & Kinzler K.W. (2021). Targeting loss of heterozygosity for cancer-specific immunotherapy. Proceedings of the National Academy of Sciences of the United States of America, 118(12), e2022410118.

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Culturing Human and Primate Cell Lines

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CHM13 cell cultured in complete AmnioMax C-100 Basal Medium (Thermo Fisher Scientific, 17001082) supplemented with 15% AmnioMax C-100 Supplement (Thermo Fisher Scientific, 12556015) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). HG00733 cells were cultured in RPMI 1640 with L-glutamine (Thermo Fisher Scientific, 11875093) supplemented with 15% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). Chimpanzee (Pan troglodytes; Clint; S006007) and macaque (Macaca mulatta; AG07107) cells were cultured in MEM α containing ribonucleosides, deoxyribonucleosides, and L-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 12% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). Orangutan (Pongo abelii; Susie; PR01109) cells were cultured in MEM α containing ribonucleosides, deoxyribonucleosides, and L-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 15% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). All cells were cultured in a humidity-controlled environment at 37°C with 5% CO₂.

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Cell Culture Conditions for Human and Primate Cells

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Cell Culture Conditions Across Species

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CHM1 cells were cultured in complete AmnioMax C-100 Basal Medium (Thermo Fisher Scientific, 17001082) supplemented with 15% AmnioMax C-100 Supplement (Thermo Fisher Scientific, 12556015) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). HG00733 (Homo sapiens) cells were cultured in RPMI-1650 media (Sigma Aldrich, R8758) supplemented with 15% fetal bovine serum (FBS; Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). Chimpanzee (Pan troglodytes; Clint; S006007) and macaque (Macaque mulatta; AG07107) cells were cultured in MEM α containing ribonucleosides, deoxyribonucleosides, and L-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 12% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). Orangutan (Pongo abelii; Susie; PR01109) cells were cultured in MEM α containing ribonucleosides, deoxyribonucleosides, and L-glutamine (Thermo Fisher Scientific, 12571063) supplemented with 15% FBS (Thermo Fisher Scientific, 16000-044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). All cells were cultured in a humidity-controlled environment at 37°C with 95% O₂.

Logsdon G.A., Rozanski A.N., Ryabov F., Potapova T., Shepelev V.A., Mao Y., Rautiainen M., Koren S., Nurk S., Porubsky D., Lucas J.K., Hoekzema K., Munson K.M., Gerton J.L., Phillippy A.M., Alexandrov I.A, & Eichler E.E. (2023). The variation and evolution of complete human centromeres. bioRxiv.

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Culturing and Differentiating Various Cell Lines

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Different types of cells were used in the current study for different purposes. Michigan Cancer Foundation-7 (MCF-7) breast cancer cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (with l-glutamine and high glucose, Gibco), supplemented with 10 %V/V fetal bovine serum (FBS) (Canada origin, ThermoFisher) and 1%V/V penicillin-streptomycin (10,000 U/mL, ThermoFisher) until 70% confluent. C2C12 myoblast cells were grown in the same DMEM, supplemented with 10%V/V heat-inactivated FBS (HI-FBS) (Canadian origin) and 1%V/V penicillin-streptomycin. For differentiation purposes, these cells were cultured in DMEM supplemented with 2%V/V of horse serum (ThermoFisher) and 1%V/V penicillin-streptomycin and 0.1% insulin (Insulin-Transferrin-Selenium, 100X, ThermoFisher, Catalogue number 41400045). SH-SY5Y neuroblastoma cells were cultured in DMEM/F-12 (ThermoFisher, with l-glutamine) medium supplemented with 10% HI-FBS and 1% penicillin-streptomycin. For differentiation of these cells, DMEM/F12 was supplemented with 1% heat-inactivated FBS, 1% N2 supplement, and 1 μM retinoic acid. Red fluorescent protein–tagged human umbilical vein endothelial cells (HUVECs) were grown in EBM-2 medium. Osteoblast-like cells from Saos-2 osteosarcoma cell line were cultured in McCoy's medium (ThermoFisher, with l-glutamine) supplemented with 15% FBS and 1% penicillin-streptomycin.

Shahin-Shamsabadi A, & Selvaganapathy P.R. (2020). Tissue-in-a-Tube: three-dimensional in vitro tissue constructs with integrated multimodal environmental stimulation. Materials Today Bio, 7, 100070.

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Cell Culture of Cancer Cell Lines

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All cell lines were cultured according to the manufacturers' instructions. Briefly, PANC-1 cells, SW480 cells, and HuH-7 cells were cultured at 37°C and 5% CO₂ in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Inc.). MIA PaCa-2 cells were maintained in DMEM with 10% FBS, 2.5% horse serum (Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin. HepG2 cells were maintained in modified Eagle's medium (Thermo Fisher Scientific, Inc.) with 10% FBS and 1% penicillin-streptomycin. Kato III cells and HuCCT1 cells were maintained in Roswell Park Memorial Institute medium 1640 (Thermo Fisher Scientific, Inc.) containing 10% FBS and 1% penicillin-streptomycin.
After incubation, the cells were washed with phosphate-buffered saline and incubated for 24 h in FBS-free medium. Then, the supernatants were centrifuged twice at 3,000 × g for 15 min and collected and divided into 6-ml aliquots and kept at −30°C until the analyses.

Yoshizawa N., Sugimoto K., Tameda M., Inagaki Y., Ikejiri M., Inoue H., Usui M., Ito M, & Takei Y. (2020). miR-3940-5p/miR-8069 ratio in urine exosomes is a novel diagnostic biomarker for pancreatic ductal adenocarcinoma. Oncology Letters, 19(4), 2677-2684.

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Isolation and Culture of Mouse Embryonic Fibroblasts

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Mouse embryos at 12.5 to 15.5 days post coitum were harvested from a pregnant female and dissected into sterile phosphate buffered saline (PBS) added with Penicillin/Streptomycin (10,000 U/mL; Thermo Fisher Scientific). After the removal of the visible internal organs and of the head, the carcasses were minced using a scalpel and digested with 0.25% trypsin-EDTA (Thermo Fisher Scientific) added with Penicillin/Streptomycin at 37 °C for 20 min. The obtained cell suspensions were transferred into 25 cm² flasks and cultured in Dulbecco’s Modified Eagle Medium (DMEM) Glutamax (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and Penicillin/Streptomycin. After the confluence, MEFs were grown in 75 cm² flasks and used in experiments at 5th passage. The galactose medium used in bioenergetics experiments is composed of DMEM without glucose (Thermo Fisher Scientific) supplemented with 10% FBS, 5 mM galactose, 5 mM pyruvate and Penicillin/Streptomycin. Cells have been grown in galactose medium for 9 days.

De Gaetano A., Gibellini L., Bianchini E., Borella R., De Biasi S., Nasi M., Boraldi F., Cossarizza A, & Pinti M. (2020). Impaired Mitochondrial Morphology and Functionality in Lonp1wt/− Mice. Journal of Clinical Medicine, 9(6), 1783.

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Establishing Cell Line Cultures for Oncological Research

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BC cell lines MDA-MB-231 and T47D were purchased from ATCC and cultured in DMEM supplemented with 10% (v/v) heat inactivated FBS and 1 × penicillin-streptomycin (containing 100 U/mL penicillin and 100 mg/mL streptomycin; all from Thermo Fisher). Mouse RAG2^−/− pre-B cell lines 63-12^{45 (link)} ectopically and stably expressing human ROR1 or human ROR2 were generated by transposition as described.^{46 (link)} Parental 63-12, 63-12/hROR1, and 63-12/hROR2 cells were cultured in IMDM supplemented with 10% (v/v) heat inactivated FBS, 0.1% (v/v) β-mercaptoethanol, and 1 × penicillin-streptomycin (all from Thermo Fisher). HEK293F cells (Thermo Fisher) were maintained in FreeStyle Medium supplemented with 1% (v/v) heat inactivated FBS to support adherent culture or without FBS for suspension culture, and 1 × penicillin-streptomycin (all from Thermo Fisher). Human chronic myelogenous leukemia cell line K562 was obtained from ATCC. A K562 cell line ectopically and stably expressing human ROR1 by retroviral transduction was kindly provided by Dr. Stanley R. Riddell (Fred Hutchinson Cancer Research Center).^{47 (link)} Both were cultured in RPMI-1640 supplemented with 10% (v/v) heat inactivated FBS and 1 × penicillin-streptomycin (all from Thermo Fisher).

Peng H., Nerreter T., Chang J., Qi J., Li X., Karunadharma P., Martinez G., Fallahi M., Soden J., Freeth J., Beerli R.R., Grawunder U., Hudecek M, & Rader C. (2017). Mining Naïve Rabbit Antibody Repertoires by Phage Display for Monoclonal Antibodies of Therapeutic Utility. Journal of molecular biology, 429(19), 2954-2973.

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